We narrowed to 10,858 results for: Kars

1. pHSV-TK

   Plasmid

   #165801

   Purpose

   Minimal viral terminator derived from Herpes Simplex 1 thymidine kinase gene

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   HSV-TK
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET24a isPETase

   Plasmid

   #225759

   Purpose

   Expresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tag

   Depositor

   Jason Boock

   Article

   Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

   Insert

   isPETase
   

   Use

   Tags

   6xHis Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCZGY2727

   Plasmid

   #193330

   Purpose

   Site specific insertion using CRISPR/Cas9 editing of C. elegans ChrI

   Depositor

   Yishi Jin

   Article

   Blazie et al Elife. 2021 Jul 29;10. pii: 68336. doi:

   Insert

   Hygromycin resistance
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Prps-0

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 4xNLS-pMJ915v2-sfGFP

   Plasmid

   #88921

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-TrxRFP1

   Plasmid

   #98996

   Purpose

   mammalian expression of a biosensor to monitor thioredoxin redox dynamics

   Depositor

   Huiwang Ai

   Article

   Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

   Insert

   human thioredoxin 1, GS-rich linker, fused with a redox-sensitive RFP
   

   Use

   Tags

   His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Beta-Lactamase

   Plasmid

   #62729

   Purpose

   Expresses Beta-Lactamase protein in bacterial cells

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   Beta-Lactamase
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Deletion of periplasmic signal domain

   Promoter

   T7

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEFIRES-P-mTagBFP-KDEL

   Plasmid

   #87163

   Purpose

   Fluorescent marker of endoplasmic reticulum (ER)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   mTagBFP-KDEL
   

   Use

   Tags

   Bip signal peptide (Homo sapiens) and mTagBFP-KDE…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1 alpha

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA-NHERF1

   Plasmid

   #31650

   Depositor

   Edward Weinman

   Article

   Weinman et al Biochemistry. 2003 Nov 4. 42(43):12662-8

   Insert

   solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)
   

   Use

   Tags

   His & S tag

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Feb. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pASTA3

   Plasmid

   #24657

   Depositor

   Tanya Parish

   Article

   Carroll et al PLoS One. 2010 . 5(3):e9823.

   Insert

   tdTomato
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21a.Tat(48-57)-EGFP

    Plasmid

    #165040

    Purpose

    Expression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)

    Depositor

    Dokyun Na

    Article

    Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

    Insert

    EGFP
    

    Use

    Tags

    6XHis and Tat(48-57)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pME-Cas9

    Plasmid

    #64237

    Purpose

    expresses codon optimized Cas9 in zebrafish

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Insert

    codon optimized Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-ECFPEYFP

    Plasmid

    #24520

    Depositor

    Liusheng He, Jehonathan Pinthus

    Article

    He et al Cytometry A. 2003 Oct . 55(2):71-85.

    Insert

    ECFP, EYFP
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pBRα

    Plasmid

    #53731

    Purpose

    Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    αNTD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp/lacUV5 (tandem promoter)

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMito-TrxRFP1

    Plasmid

    #98997

    Purpose

    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, mitochondrially locaclized

    Depositor

    Huiwang Ai

    Article

    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

    Insert

    TrxRFP1 and mitochondrial localization sequence
    

    Use

    Tags

    His-tag and Mitochondria localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pU6a:sgRNA(tyr)

    Plasmid

    #64250

    Purpose

    expresses sgRNA(tyr) under U6a promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Insert

    U6a:sgRNA (tyr)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCHARGE3

    Plasmid

    #24658

    Depositor

    Tanya Parish

    Article

    Carroll et al PLoS One. 2010 . 5(3):e9823.

    Insert

    Turbo
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. V309 - pDNR MCS SA lacZ alpha - Donor

    Plasmid

    #11692

    Depositor

    Tony Pawson

    Article

    Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

    Type

    Empty backbone

    Use

    Cre/Lox; Donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. DHFR-myc

    Plasmid

    #20214

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    DHFR (folA E. coli)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-cdc2-AF (718)

    Plasmid

    #39872

    Depositor

    Jonathon Pines

    Article

    Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

    Insert

    Cdc2 (CDK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    AF = T14A, Y15F

    Promoter

    CMV

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pZ149-pcDNA3-BhCas12b_v4

    Plasmid

    #122446

    Purpose

    Expresses BhCas12b_v4 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    CMV-NLS-BhCas12b_v4-NLS-3xHA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge