We narrowed to 2,341 results for: PUC19

1. pMB41

   Plasmid

   #73087

   Purpose

   N-terminal Avi-2xTEV-GFP vector, for fosmid recombineering

   Depositor

   Mike Boxem

   Article

   Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

   Insert

   Avi-GFP tag
   

   Tags

   Avi and GFP

   Expression

   Worm

   Available Since

   July 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMB72

   Plasmid

   #73088

   Purpose

   C-terminal GFP-2xTEV-Avi vector, for fosmid recombineering

   Depositor

   Mike Boxem

   Article

   Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

   Insert

   GFP-Avi tag
   

   Tags

   GFP and Avi

   Expression

   Worm

   Available Since

   July 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. UPRT-mCh-Nluc-P2A-neo-UPRT

   Plasmid

   #135015

   Purpose

   Expresses mCherry protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum UPRT locus

   Depositor

   David Sibley

   Article

   Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

   Inserts

   mCh-Nluc-P2A-neo
   UPRT 5'UTR
   UPRT 3'UTR
   

   Use

   Cryptosporidium expression

   Tags

   mCherry and Nluc-neo

   Promoter

   Cryptosporidium actin and enolase

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. LADL Bridge + Target Zfp462-Klf4SE

   Plasmid

   #127668

   Purpose

   Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1a and hU6

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAP1892-1

   Plasmid

   #105247

   Purpose

   human GFP11 with tagRFP 33bp downstream in Lamin A/C

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

   Insert

   Insertion of GFP11 at cut tagRFP 33bp downstream in Lamin A/C (LMNA Human)
   

   Expression

   Bacterial

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. LADL Bridge + Promoter Only Target

   Plasmid

   #127669

   Purpose

   Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promoters

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   CRY2-HA-2A-mCherry and gRNAs 115 and 117
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1a and hU6

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDV12 [psng-1::SNG-1::CRY2olig(535)]

   Plasmid

   #197596

   Purpose

   Pan-neuronal expression of SNG-1::CRY2olig(535) in neurons of C. elegans

   Depositor

   Alexander Gottschalk

   Article

   Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

   Insert

   Synaptogyrin-CRY2 (E490G + residues 1-535) (CRY2, sng-1 Mustard Weed, Nematode)
   

   Expression

   Worm

   Mutation

   E490G

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIRHD-TetR-GAL80-Rho1-GAL4

   Plasmid

   #218274

   Purpose

   Introduction of low-temperature-inducible GAL system, LowTempGAL v2.2

   Depositor

   Christopher Howard

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   gal80Arm1-pAgTEF1>KanMX4>tAgTEF1-pHAC1>UBI4>>DHFRP66L-TetR>NLS>TUP1>tABF1-pTEF2>Rho1C17R>>GAL4>tGAL4-pTEF1(TetO)>GAL80Arm2
   

   Expression

   Yeast

   Mutation

   DHFR (P66L), Rho1 (C17R)

   Available Since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIRHDGAL43C

   Plasmid

   #218279

   Purpose

   Introduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)
   

   Expression

   Yeast

   Mutation

   GAL3 (S509P), DHFR (P66L)

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIRGAL43C

    Plasmid

    #218278

    Purpose

    Introduction of a LowTempGAL Turbo module (GAL43C) to accelerate induction of GAL promoter induction

    Depositor

    Claudia Vickers

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
    

    Expression

    Yeast

    Mutation

    GAL3 (S509P)

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIRHDGAL3C

    Plasmid

    #218276

    Purpose

    Introduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter induction

    Depositor

    Claudia Vickers

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
    

    Expression

    Yeast

    Mutation

    GAL3 (S509P), DHFR (P66L)

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIRGAL3C

    Plasmid

    #218275

    Purpose

    Introduction of a LowTempGAL Turbo module (GAL3C) to accelerate induction of GAL promoter induction

    Depositor

    Claudia Vickers

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
    

    Expression

    Yeast

    Mutation

    GAL3 (S509P)

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Donor_YTHDF2_KO_Neo

    Plasmid

    #186672

    Purpose

    Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. Neomycin and polyA sequence is inserted between two homology arms.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    YTHDF2 KO Neomycin (YTHDF2 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Donor_ANKRD1_KO_Hygro

    Plasmid

    #186668

    Purpose

    Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    ANKRD1 KO Hygromycin (ANKRD1 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNOC_dfnCas12a-KRAB_crRNA Td2

    Plasmid

    #176261

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    Kruppel associated box domain and luciferase

    Mutation

    D917A and E1006A mutations to inactivate the endo…

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIFU002

    Plasmid

    #174299

    Purpose

    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the N501Y mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Insert

    Spike RBD N343Q, N501Y (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    HA and cMyc

    Expression

    Yeast

    Mutation

    N343Q, N501Y

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIFU001

    Plasmid

    #174298

    Purpose

    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the E484K mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Insert

    Spike RBD N343Q, E484K (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    HA and cMyc

    Expression

    Yeast

    Mutation

    N343Q, E484K

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAP1893-1

    Plasmid

    #105248

    Purpose

    human GFP11 with tagRFP 33bp downstream in Lamin A/C (recoded)

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

    Insert

    Insertion of GFP11 at cut tagRFP 33bp downstream (recoded *) in Lamin A/C (LMNA Human)
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIG-601_NY-ESO-1_TCR_GFP

    Plasmid

    #207482

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    GFP, NY-ESO-1_TCR
    

    Use

    CRISPR

    Mutation

    Potential silent point mutation in GFP sequence

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-TRE-OKSM

    Plasmid

    #204498

    Purpose

    Expresses Oct4, Klf4, Sox2 and c-Myc by tet-on promoter in mammalian cells

    Depositor

    Wataru Fujii

    Article

    Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

    Inserts

    OCT4 (POU5F1 Human)
    KLF4 (KLF4 Human)
    SOX2 (SOX2 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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