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FKBP-beta2(616-951)-GFP ∆CBM

Plasmid

#100728

Purpose

To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Long isoform, deletion of clathrin box motif

Promoter

CMV

Available Since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pPRIME-CMV-GFP-shCrh1

Plasmid

#132709

Purpose

Encodes short hairpin RNA (shRNA) #1 that targets the 3’-untranslated region of the rat Crh gene

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

shCrh(1) (Crh Rat)

Use

Lentiviral

Promoter

CMV

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-mkgDUX4mal*mqg*

Plasmid

#21174

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Insert

DUX4 (DUX4 Human)

Expression

Bacterial and Mammalian

Mutation

First point mutation at nucleotide 5114 (numberin…

Available Since

Aug. 6, 2009

Availability

Academic Institutions and Nonprofits only
pBABE MD-deltaN-ER

Plasmid

#13496

Depositor

Stephen Tapscott

Article

Berkes et al Mol Cell. 2004 May 21. 14(4):465-77.

Insert

MyoD1-Estrogen Receptor Fusion Protein (Myod1 Human, Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

MyoD (containing a deletion in aa3-56) was fused …

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
FKBP-beta2(616-951)-GFP Y215A

Plasmid

#100727

Purpose

To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Long isoform Y215A mutation (Y815A in full length)

Promoter

CMV

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pBrain-GFP-TACC3KDP∆678-681-shTACC3

Plasmid

#69115

Purpose

Dual-promoter plasmid to express knockdown-proof human TACC3 (amino acids 678-681 deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

Depositor

Stephen Royle

Article

Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

Inserts

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
shTACC3

Use

RNAi

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of 678-681aa region. Silent mutations i…

Promoter

CMV

Available Since

Oct. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCBS-4800

Plasmid

#249323

Purpose

To express the EPEC DarT2(DLAA)-nScCas9(D10A) fusion protein in experiments for Append Editing

Depositor

Chase Beisel

Article

Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038

Insert

EPEC DarT2(DLAA)-nScCas9(D10A)

Expression

Bacterial

Mutation

DarT2(G49D, M86L, R92A, R193A) and nScCas9(D10A)

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCBS-6738

Plasmid

#249322

Purpose

To express the EPEC DarT2(G49D)-nScCas9(D10A) fusion protein in experiments for Append Editing

Depositor

Chase Beisel

Article

Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038

Insert

EPEC DarT2(G49D)-nScCas9(D10A)

Expression

Bacterial

Mutation

DarT2(G49D) and nScCas9(D10A)

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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TRBV7-4

Plasmid

#237923

Purpose

Encodes TRBV7-4 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBV7-4-TRAC (TRBV7-4 Human)

Use

Synthetic Biology

Mutation

None

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV7-8

Plasmid

#237926

Purpose

Encodes TRBV7-8 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBV7-8-TRAC (TRBV7-8 Human)

Use

Synthetic Biology

Mutation

None

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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