We narrowed to 4,227 results for: Pet2

1. pET28a-DDX43-K292A

   Plasmid

   #134575

   Purpose

   Express human DDX43 mutant (K292A, ATPase dead) in E. coli

   Depositor

   Yuliang Wu

   Article

   Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

   Insert

   DDX43-K292A (DDX43 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   full-length (K292A)

   Promoter

   T7

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-SUMO-KaiA

   Plasmid

   #68049

   Purpose

   Strain: AM5115, Used for protein purification

   Depositor

   Susan Golden

   Article

   Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

   Insert

   KaiA
   

   Use

   Tags

   6xHis and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28_6xHis-PARP1delWGR-L713F

   Plasmid

   #173941

   Purpose

   Bacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delWGR-L713F (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 521-660; L713F, V762A

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21-pelB-LaG-27

   Plasmid

   #172752

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-27
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a_6HT-TBP6.7(R49A)

   Plasmid

   #112726

   Purpose

   Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R49A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R49A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. eGFP-Silicatein-pET28

   Plasmid

   #198012

   Purpose

   C-terminal eGFP fusion to truncated silicatein insert with N- and C-terminal hexahistidine tag for purification

   Depositor

   Bryan Berger

   Article

   Vigil et al Materials Advances 2, 2023

   Insert

   eGFP-silicatein
   

   Use

   Tags

   hexahistidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-PKM2-G415R

   Plasmid

   #122693

   Purpose

   Express His-tagged PKM2 (G415R) protein in bacteria.

   Depositor

   Wen-Ching Wang

   Article

   Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

   Insert

   Pyruvate kinase M2 isoform (PKM Human)
   

   Use

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   G415R

   Promoter

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-p53 (1-73) (72P)

   Plasmid

   #62083

   Purpose

   Expression of human p53 (1-73) (72P) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   HIs

   Expression

   Bacterial

   Mutation

   contains TAD residues 1-73; 72P

   Promoter

   T7

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pET28b(+)-SPN+IFS

   Plasmid

   #83372

   Purpose

   Expresses SPN and IFS complex in E. coli cell

   Depositor

   SeWon Suh

   Article

   Yoon et al J Synchrotron Radiat. 2013 Nov;20(Pt 6):

   Inserts

   SPN
   IFS
   

   Use

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-MRPP3-His

    Plasmid

    #67866

    Purpose

    bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tag

    Depositor

    Walter Rossmanith

    Article

    Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

    Insert

    MRPP3 (KIAA0391 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino acid 46 of recombinant MRPP3 is preceded by…

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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