We narrowed to 5,869 results for: nls

1. pSpG-PPE

   Plasmid

   #170130

   Purpose

   For plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

   Insert

   nCas9-SpG(H840A)-M-MLV
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   H840A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T1…

   Promoter

   maize Ubiquitin-1

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSBbi-FoxO1_1R_13A_3D

   Plasmid

   #106279

   Purpose

   Fluorescent fusion protein for FoxO1

   Depositor

   Laura Heiser

   Article

   Gross et al Cell Syst. 2019 Dec 18;9(6):580-588.e4.

   Inserts

   Foxo1a (Foxo1 Mouse)
   NLS-mCherry-NLS-P2A-Puromycin
   

   Use

   Tags

   Clover fluorescent protein and NLS

   Expression

   Mammalian

   Mutation

   Deleted amino acids 401-636, T24A, S209A, H212R, …

   Promoter

   EF1a/RPBSA

   Available since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3s-ABE7.10

   Plasmid

   #113128

   Purpose

   expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized

   Depositor

   Jin-Soo Kim

   Article

   Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

   Insert

   ABE 7.10
   

   Use

   Tags

   HA epitope and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pROF141

   Plasmid

   #155329

   Purpose

   Binary vector for expression of the PULSE system under the control of PAtUbi10 promoter

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   LB_PAtUbi10-SRDX-NLS-EL222-Tnos_SF_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQCas12j2

   Plasmid

   #189780

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS, without promoter

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Insert

   Cas12j2
   

   Use

   CRISPR; Gateway compatible cas12j2 entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-Flag-SpCas9-HF1 (without sgRNA)

   Plasmid

   #92102

   Purpose

   Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   Cbh

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. eIscB

   Plasmid

   #227016

   Purpose

   Vector encoding human codon-optimized enhanced-activity OgeuIscB fusion with extra SV40 NLS driven by CMV promoter

   Depositor

   Dali Li

   Article

   Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

   Insert

   SV40 NLS-OgeuIscB-v3-SV40-nucleoplasmin NLS
   

   Use

   Tags

   Expression

   Mutation

   D96R/E84R/V159R

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPS1888

   Plasmid

   #1360

   Depositor

   Pamela Silver

   Article

   Damelin et al Mol Cell 2000 Jan;5(1):133-40.

   Insert

   NLS-EYFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

9. pPS1890

   Plasmid

   #1362

   Depositor

   Pamela Silver

   Article

   Damelin et al Mol Cell 2000 Jan;5(1):133-40.

   Insert

   NLS-ECFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pPS1891

    Plasmid

    #1363

    Depositor

    Pamela Silver

    Article

    Damelin et al Mol Cell 2000 Jan;5(1):133-40.

    Insert

    NLS-EYFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pMLS285

    Plasmid

    #73734

    Purpose

    SapTrap egl-13 NLS connector donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    egl-13 NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. DECOR

    Plasmid

    #219545

    Purpose

    Expression of CMV-TadA8e-NLS-dCas13d-NLS in mammalian cells (DECOR expression)

    Depositor

    Weixin Tang

    Article

    Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

    Insert

    DECOR
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLH121

    Plasmid

    #22696

    Depositor

    Lynn Hudson

    Article

    Nadon et al J Neurochem. 1994 Sep . 63(3):822-33.

    Insert

    Human Proteolipid Protein cDNA (PLP1 Human)
    

    Use

    Phagemid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pVITRO1-SS-269

    Plasmid

    #68771

    Purpose

    Sensor pair 1 V2

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Inserts

    C-Terminal Sensor 1 V2
    N-Terminal Sensor 1 V2
    

    Use

    Tags

    NLS, flag, and nls

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRM2

    Plasmid

    #8486

    Depositor

    Nancy Craig

    Article

    McKown et al J Bacteriol 1988 Jan;170(1):352-8.

    Insert

    attTn7 (tnsA Bacteria)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. L1P80

    Plasmid

    #195941

    Purpose

    Level 1 pLBD16 reporter : pLBD16-mScarlet-NLS-tRBCS

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    pLBD16-mScarlet-NLS-tRBCS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. L1P79

    Plasmid

    #195940

    Purpose

    Level 1 pGATA23 reporter : pGATA23-mScarlet-NLS-tRBCS

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    pGATA23-mScarlet-NLS-tRBCS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. lvl0 mKate2 CDS

    Plasmid

    #153394

    Purpose

    Far red fluorescent protein with SV40 NLS, codon optimised for Arabidopsis thaliana

    Depositor

    Naomi Nakayama

    Article

    Andreou et al bioRxiv, March 31, 2021

    Insert

    mKate2 CDS
    

    Use

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVITRO1-SS-267

    Plasmid

    #68770

    Purpose

    Sensor pair 1 V1

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Inserts

    C-terminal Sensor 1 V1
    N-Terminal Sensor 1 V1
    

    Use

    Tags

    NLS, flag, and nls

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVITRO1-SS-271

    Plasmid

    #68772

    Purpose

    Sensor pair 1 V3

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Inserts

    C-Terminal Sensor 1 V3
    N-Terminal Sensor 1 V3
    

    Use

    Tags

    NLS, flag, and nls

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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