We narrowed to 18,379 results for: Met

1. pGreen2x35SLUC

   Plasmid

   #108228

   Purpose

   Plant expression as translational fusions to FLUC with RLUC normalization

   Depositor

   Alejandro Ferrando

   Article

   Solé-Gil et al Methods Mol Biol. 2024;2722:79-87. doi:

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Firefly Luciferase

   Expression

   Plant

   Promoter

   2x35S

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP

   Plasmid

   #31484

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   EF1s HA NLS Sce(opt) T2A IFP1.4
   

   Use

   Lentiviral

   Tags

   HA and NLS

   Mutation

   "Opt" indicates the I-SceI coding seque…

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. Str-Ii_VSVG-SBP-EGFP

   Plasmid

   #65300

   Purpose

   synchronize trafficking of VSVG from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   VSV-G
   

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Str-Ii_VSVG-SBP-mCherry

   Plasmid

   #65301

   Purpose

   synchronize trafficking of VSVG from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   VSV-G
   

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSFDuet-1_Uricase

   Plasmid

   #182400

   Purpose

   Bacterial expression of uricase

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   uricase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 APEX2-NES

   Plasmid

   #49386

   Purpose

   Expresses APEX2 in the cytosol of mammalian cells

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   Flag - APEX2 - NES
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K14D, W41F, E112K, A134P on soybean APX; improved…

   Promoter

   CMV

   Available Since

   Nov. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 Connexin43-GFP-APEX2

   Plasmid

   #49385

   Purpose

   Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cells

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   Connexin43 - EGFP - APEX2
   

   Expression

   Mammalian

   Mutation

   K14D, W41F, E112K, A134P on soybean APX; improved…

   Promoter

   CMV

   Available Since

   Nov. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-G-Creon shRNA[Control]

   Plasmid

   #181824

   Purpose

   This vector simultaneously expresses GFP protein and shRNA in a Cre-dependent manner.

   Depositor

   Eun Mi Hwang

   Article

   Kim et al J Neurosci Methods. 2021 Dec 21:109452.

   Type

   Empty backbone

   Use

   AAV and RNAi

   Expression

   Mammalian

   Promoter

   EF1a/U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC_pThrRS_SeptRNAv2.0CUA_OXB20-PduX_EFSep

   Plasmid

   #173899

   Purpose

   The pThr/tRNAv2.0CUA pair enables the biosynthesis of proteins containing pThr

   Depositor

   Jason W Chin

   Article

   Zhang et al Nat Methods. 2017 Jul;14(7):729-736. doi

   Inserts

   ThrRS(v1.0)
   tRNA(v2.0)CUA
   pduX
   EF-Sep
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. mRuby2-N1

    Plasmid

    #54614

    Purpose

    Localization: N1 Cloning Vector, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Type

    Empty backbone

    Tags

    mRuby2

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pMK344 (Hygro-P2A-mAID)

    Plasmid

    #121178

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID
    

    Use

    CRISPR

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRE-EGFP

    Plasmid

    #89871

    Purpose

    EGFP reporter vector for iTango system

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    tetO-EGFP
    

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCFJ601 - Peft-3 Mos1 transposase

    Plasmid

    #34874

    Purpose

    Mos1 transposase expression vector for use in creating targeted transgene insertions and targeted deletions in C. elegans

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    Mos1 transpoase
    

    Use

    Worm targeting

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDD104 (Peft-3::Cre)

    Plasmid

    #47551

    Purpose

    Cre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germline

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

    Insert

    Cre Recombinase
    

    Use

    Cre/Lox

    Expression

    Worm

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. piRFP670-N1

    Plasmid

    #45457

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    iRFP670
    

    Expression

    Mammalian

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMito-iRFP670

    Plasmid

    #45462

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP670
    

    Tags

    human cytochrome C oxidase subunit VIII

    Expression

    Mammalian

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTriEx-mCherry-LOV2

    Plasmid

    #81041

    Purpose

    Mammalian or insect expression for wt LOV2

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV2
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV, p10

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Str-KDEL_neomycin

    Plasmid

    #65306

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
    

    Tags

    KDEL motif and Signal Sequence

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBAMD1-2

    Plasmid

    #61564

    Purpose

    Standardized mini-Tn5 delivery plasmid, kanamycin resistant

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

    Type

    Empty backbone

    Use

    Synthetic Biology; Mini-tn5 vector

    Available Since

    Jan. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-EF1a-BbChT

    Plasmid

    #45186

    Depositor

    Dawen Cai, Joshua Sanes

    Article

    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

    Has Service

    AAV9

    Insert

    pAAV-Ef1a-Brainbow/mCherry/mTFP-WPRE
    

    Use

    AAV; Adeno-associated viral

    Expression

    Mammalian

    Promoter

    human EF1a

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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