We narrowed to 9,259 results for: pho

1. pCMV5 Flag-Smad1 (4SP/AP)

   Plasmid

   #14043

   Depositor

   Joan Massague

   Article

   Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

   Insert

   Smad1 (4SP/AP) (SMAD1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Four Serine to Alanine mutations in the linker re…

   Promoter

   Available since

   May 10, 2007

   Availability

   Academic Institutions and Nonprofits only

2. Dynamin I-K44A-non-tagged

   Plasmid

   #32695

   Depositor

   Robert Lefkowitz

   Article

   Ahn et al J Biol Chem. 2002 Jul 19;277(29):26642-5

   Insert

   Dynamin I (Dnm1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K44A

   Promoter

   CMV

   Available since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

3. FLAG-β-arrestin2 RRK/Q

   Plasmid

   #38261

   Depositor

   Robert Lefkowitz

   Article

   Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

   Insert

   β-arrestin2 (Arrb2 Rat)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Arg233, Arg237, and Lys251 converted to Glutamines

   Promoter

   CMV

   Available since

   Aug. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pRRL-VinculinVenus-S1033A

   Plasmid

   #211896

   Purpose

   Vinculin (chicken) with S1033 unphosphorylatable point mutation (S1033A) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.

   Depositor

   Brenton Hoffman

   Article

   Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12

   Insert

   VinculinVenus-S1033A (VCL Chicken)
   

   Use

   Lentiviral

   Tags

   VenusA206K

   Expression

   Mutation

   mutated vinculin serine 1033 to alanine (S1033A),…

   Promoter

   CMV

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRRL-VinculinVenus-S1033D

   Plasmid

   #211897

   Purpose

   Vinculin (chicken) with S1033 phosphomimetic point mutation (S1033D) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.

   Depositor

   Brenton Hoffman

   Article

   Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12

   Insert

   VinculinVenus-S1033D (VCL Chicken)
   

   Use

   Lentiviral

   Tags

   VenusA206K

   Expression

   Mutation

   mutated vinculin serine 1033 to aspartic acid (S1…

   Promoter

   CMV

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUAST-SIK3 T196A

   Plasmid

   #52974

   Purpose

   insect expression of SIK3 T196A (LKB1 phosphorylation defective mutant)

   Depositor

   Marc Montminy, Biao Wang

   Article

   Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

   Insert

   SIK3 (Sik3 Fly)
   

   Use

   Tags

   HA

   Expression

   Insect

   Mutation

   T196A LKB1 phosphorylation defective mutant

   Promoter

   Hsp70

   Available since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pSG5-Myr-FLAG-p110α-DM

   Plasmid

   #55713

   Purpose

   eukaryotic expression of myristoylation tag (Myr-FLAG) p110a RBD double mutant T207D, K227A

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   p110 alpha (Pik3ca Mouse)
   

   Use

   Tags

   myristoylation tag (Myr-FLAG)

   Expression

   Mammalian

   Mutation

   T207D, K227A

   Promoter

   T7

   Available since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pGEX Supt6h-SH2

   Plasmid

   #46518

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Supt6h (SUPT6H Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1317-1441

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCIneoLuc-PP1A

   Plasmid

   #128348

   Purpose

   Vector for LUMIER assay

   Depositor

   Yutaka Hata

   Article

   Kawano et al J Biochem. 2015 May 14. pii: mvv051.

   Insert

   PP1A (PPP1CA Human)
   

   Use

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-4T1-Claspin-MΔN

    Plasmid

    #31624

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

    Insert

    Claspin (CLSPN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 899-1206 only

    Promoter

    Available since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

11. GB1-PRD(800-868)-strep

    Plasmid

    #180023

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    GB1-His6-TEV-ALIX(800-868)-Strep (PDCD6IP Human)
    

    Use

    Tags

    GB1-His6-TEV and Strep tag (WSHPQFEK)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-CMV-cGASΔN-HA (murine)

    Plasmid

    #130915

    Purpose

    Expresses murine cGASΔN (aa148-507)-HA; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    cGASΔN murine
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    murine cGAS catalytic domain (aa148-507)

    Promoter

    CMV

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX Nck-SH2

    Plasmid

    #46457

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Nck (NCK1 Human)
    

    Use

    Tags

    GST and Thrombin site

    Expression

    Bacterial

    Mutation

    contains amino acids 275-377

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. shNg Lenti FHRC3J1UGW

    Plasmid

    #92233

    Purpose

    lentiviral expression of EGFP and Ng shRNA

    Depositor

    Weifeng Xu

    Article

    Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

    Insert

    shRNA targeting Ng
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX ShcA-SH2

    Plasmid

    #46486

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    ShcA (SHC1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 476-584

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-C1 SNX9 KR mutant K267N R327N

    Plasmid

    #161994

    Purpose

    Mammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tag

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    SNX9 (Snx9 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    K267N R327N

    Promoter

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLJM60-S6K1(T389S) barcoded

    Plasmid

    #48800

    Purpose

    expresses mutant S6K1

    Depositor

    David Sabatini

    Article

    Kang et al Science. 2013 Jul 26;341(6144):1236566.

    Insert

    S6K1 T389S (Rps6kb1 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG and barcode: GGTACC

    Expression

    Mammalian

    Mutation

    T389S

    Promoter

    CMV

    Available since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. PKM-IRES-hrGFP

    Plasmid

    #73405

    Purpose

    Mammalian expression of mouse PKM and hrGFP

    Depositor

    Raymond Swanson

    Article

    Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

    Insert

    PKM (Prkcz Mouse)
    

    Use

    Tags

    3xFLAG and pIRES-hrGFP-2a

    Expression

    Mammalian

    Mutation

    truncated, constitutively active form

    Promoter

    CMV

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA Flag-RARalpha (T181A,S445A,S461A)

    Plasmid

    #35556

    Depositor

    Jonathan Kurie, Harish Srinivas

    Article

    Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

    Insert

    RARalpha (RARA Human)
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    T181A, S445A, S461A

    Promoter

    Available since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pGEX-4T Doc2beta C2AB(125) WT

    Plasmid

    #134690

    Purpose

    Encodes cytoplasmic domain of Doc2beta for bacterial expression and purification

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Double C2 protein beta (Doc2b Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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