161,851 results

1. pMXs-puro GFP-p62

   Plasmid

   #38277

   Depositor

   Noboru Mizushima

   Article

   Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

   Insert

   Sequestosome-1 (Sqstm1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLJM1 Raptor GFP

   Plasmid

   #112744

   Purpose

   express Raptor-GFP

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Raptor-GFP (RPTOR Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDule-tfmF A65V S158A

   Plasmid

   #85484

   Purpose

   Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

   Insert

   tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32V F108W Q109M I159M with 65Val and 158Ala

   Promoter

   lpp (constitutive)

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLSC-5

   Plasmid

   #62889

   Purpose

   Lenti vector for expression of inducible split Cas9

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Inserts

   SpCas9(574-1368)
   SpCas9(2-573)
   

   Use

   CRISPR and Lentiviral

   Tags

   FFBP12, FRB, NES PTK2, NLS SV40, and P2A

   Expression

   Mammalian

   Mutation

   *see comment below

   Promoter

   EFS

   Available since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSP1

   Plasmid

   #20060

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

   Insert

   MSP1 (APOA1 synthetic gene)
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   synthetic gene of deletion mutant (1-43) of human…

   Promoter

   Available since

   Jan. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-EF1-rtTA-PGK-BSD

   Plasmid

   #192885

   Purpose

   expression of rtTA

   Depositor

   Kazuhiro Oka

   Insert

   rtTA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGs-BFP

   Plasmid

   #127348

   Purpose

   Expresses BFP constitutively via the CAGGS promoter in mammalian cells

   Depositor

   Phil Sharp

   Insert

   Blue Fluorescent Protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS Promoter

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-Sec23A

   Plasmid

   #166894

   Purpose

   mammalian expression of Sec23A tagged with mCherry

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

   Insert

   SEC23A (SEC23A Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Mito-B

   Plasmid

   #209864

   Purpose

   Dimerization dependent fluorescent protein B anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVS

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP B
   

   Use

   Tags

   Targeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. ER-GA

    Plasmid

    #209871

    Purpose

    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP A
    

    Use

    Tags

    Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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