We narrowed to 4,850 results for: U6...

1. px459-Pole3 KO sgRNA

   Plasmid

   #186934

   Purpose

   Pole3 KO in mouse ES cells

   Depositor

   Zhiguo Zhang

   Article

   Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

   Insert

   Pole3 KO sgRNA (Pole3 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX459-HypaCas9-CfANLN_sgRNA

   Plasmid

   #183880

   Purpose

   pX459V2.0-HypaCas9 plasmid with cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   Canis familiaris ANLN sgRNA spacer
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX459-sg-tdTomato166/892

   Plasmid

   #179919

   Purpose

   Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 166 and 892.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   tdTomato sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX552-mScn8a-3xHA-Syn-smFP-flag

   Plasmid

   #182563

   Purpose

   For mouse Nav1.6 knockin with 3xHA at the C-terminal

   Depositor

   James Zhe Liu

   Article

   Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

   Inserts

   mouse Scn8a gRNA and 3xHA donor DNA
   smFP-flag
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   EF1 and U6

   Available since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX552-mScn8a-HaloTag-V5-EF1-sfGFP

   Plasmid

   #182565

   Purpose

   For mouse Nav1.6 knockin with HaloTag-V5 at the C-terminal

   Depositor

   James Zhe Liu

   Article

   Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

   Inserts

   mouse Scn8a gRNA and HaloTag-V5 donor DNA
   sfGFP
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   EF1 and U6

   Available since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX552-mScn8a-3xV5-EF1-smFP-flag

   Plasmid

   #182562

   Purpose

   For mouse Nav1.6 knockin with 3xV5 at the C-terminal

   Depositor

   James Zhe Liu

   Article

   Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

   Inserts

   mouse Scn8a gRNA and 3xV5 donor DNA
   smFP-flag
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   EF1 and U6

   Available since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJJF449_dpy-10_CDS_sg1

   Plasmid

   #163866

   Purpose

   sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJR50.

   Depositor

   Nikolaus Rajewsky

   Article

   Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

   Insert

   sgRNA targeting the coding sequence of dpy-10
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   R07E5.16 (U6)

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJJF328_sqt-3_3′UTR_sg2

   Plasmid

   #163867

   Purpose

   sgRNA targeting sqt-3 (3′UTR) in C. elegans. Backbone: pJJR50.

   Depositor

   Nikolaus Rajewsky

   Article

   Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

   Insert

   sgRNA targeting the 3'UTR of sqt-3
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   R07E5.16 (U6)

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJJF496_dpy-10_CDS_sg6

   Plasmid

   #164267

   Purpose

   sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJR50.

   Depositor

   Nikolaus Rajewsky

   Article

   Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

   Insert

   sgRNA targeting the coding sequence of dpy-10
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   R07E5.16 (U6)

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJJF495_dpy-10_CDS_sg6

    Plasmid

    #164268

    Purpose

    sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJF439.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    sgRNA targeting the coding sequence of dpy-10
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6 promoter from W05B2.8

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. sg2

    Plasmid

    #113967

    Purpose

    Single short guide RNA targeting TACCACATTTGTAGAGGTT

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. sg3

    Plasmid

    #113968

    Purpose

    Single short guide RNA targeting CAATGTATCTTATCATGTC

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. sg2+sg3

    Plasmid

    #113970

    Purpose

    Double short guide RNA targeting TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg2+sg3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-hsg(NT:NT)

    Plasmid

    #138260

    Purpose

    Expression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    Nontargeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCZGY2750

    Plasmid

    #135094

    Purpose

    Site specific CRISPR/Cas9 editing of C. elegans Chr IV

    Depositor

    Yishi Jin

    Article

    Andrusiak et al Neuron. 2019 Jul 24. pii: S0896-6273(19)

    Insert

    sgRNA for cxTi10082 (actgttggatgcctgtgtag)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX330_ZBTB7B_2

    Plasmid

    #135747

    Purpose

    Encodes gRNA for 3' target of human ZBTB7B

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZBTB7B gRNA (ZBTB7B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PX330_ZBTB7B_1

    Plasmid

    #135746

    Purpose

    Encodes gRNA for 3' target of human ZBTB7B

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZBTB7B gRNA (ZBTB7B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. px330-MRPsg3

    Plasmid

    #87191

    Purpose

    disruption of human RMRP gene via CRISPR-Cas9, guide 3

    Depositor

    Thomas Cech

    Article

    Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

    Insert

    human MRP RNA CRISPR guide #3 (RMRP Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiCRISPR - C16orf80 sgRNA 1

    Plasmid

    #70651

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against C16orf80
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

20. lentiCRISPR - C16orf80 sgRNA 2

    Plasmid

    #70650

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against C16orf80
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only