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We narrowed to 2,306 results for: puc19

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P532_POU4F2-p2A-tdTomato-p2A-Thy1.2

Plasmid

#239102

Purpose

Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of POU4F2 (aka BRN3B). This includes the donor casette and a U6 driven guide sequence to be used with Cas9.

Depositor

Karl Wahlin

Article

Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472

Insert

POU4F2 (POU4F2 Human)

Use

Donor plasmid for integration into the human geno…

Tags

p2A-tdTomato-p2A-Thy1.2

Expression

Mammalian

Mutation

Promoter

Endogenous POU4F2

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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P527_POU4F2-p2A-mNeonGreen-h2b

Plasmid

#239103

Purpose

Azenta NGS; inserts a p2A-mNeonGreen-h2b fusion at the endogenous POU4F2 (aka BRN3B) gene

Depositor

Karl Wahlin

Article

Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

Insert

POU4F2 (POU4F2 Human)

Use

Tags

p2A-mNeonGreen-h2b

Expression

Mammalian

Mutation

Promoter

Endogenous POU4F2

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pYX-N-Puro-mNeon-TSC22D1

Plasmid

#222667

Purpose

endogenously tag TSC22D1 with N-terminal mNeon

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

TSC22D1 (TSC22D1 Human)

Use

Tags

mNeon

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pYX-N-Blast-miniTurbo-TSC22D2

Plasmid

#222669

Purpose

endogenously tag TSC22D2 with N-terminal miniTurbo

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

TSC22D2 (TSC22D2 Human)

Use

Tags

miniTurbo

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-748_HA-GD2-28z_CAR_BATF

Plasmid

#207488

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

BATF, HA-GD2-28z_CAR (BATF Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-805_HA-GD2-28z_CAR_BATF-RFP

Plasmid

#207492

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

BATF-RFP, HA-GD2-28z_CAR (BATF Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-755_HA-GD2-28z_CAR_RFP-JUN

Plasmid

#207494

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

RFP-JUN, HA-GD2-28z_CAR (JUN Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-837_NY-ESO-1_TCR_tFAS

Plasmid

#207500

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

tFAS, NY-ESO-1_TCR (FAS Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTR-378 tNGFR-P2A-CCR7

Plasmid

#186072

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CCR7 (CCR7 Human)

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-450 tNGFR-P2A-CXCR4

Plasmid

#186078

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CXCR4 (CXCR4 Human)

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTPT

Plasmid

#1962

Depositor

Robert Kingston

Article

Sif et al Genes Dev 1998 Sep 15;12(18):2842-51.

Insert

TPT

Use

Tags

Expression

Bacterial

Mutation

2 copies of 20bp artificial nucleosome positionin…

Promoter

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pWAP-HGF

Plasmid

#83503

Purpose

Generation of transgenic mice overexpressing the HGF under the control of the WAP gene promoter

Depositor

Lothar Hennighausen, Marta Gallego

Article

Gallego et al Oncogene. 2003 Nov 20;22(52):8498-508.

Insert

HGF (Hgf Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

also contains beta-globin sequences from pUC198

Promoter

mouse WAP (whey acidic protein)

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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NYESO beta/alpha into TRAC HDRT Source (pTR 169)

Plasmid

#112021

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO beta/alpha into TRAC HDRT

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 1, 2018

Availability

Academic Institutions and Nonprofits only

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NYESO alpha/beta into TRBC1 HDRT Source (pTR 262)

Plasmid

#112022

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-beta

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO alpha/beta into TRBC1 HDRDT

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2018

Availability

Academic Institutions and Nonprofits only

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NYESO alpha into TRAC HDRT Source (pTR 223)

Plasmid

#112023

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alpha

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO alpha into TRAC HDRT

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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NYESO beta into TRBC1 HDRT Source (pTR 277)

Plasmid

#112024

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR-beta with 1G4 NYESO TCR-beta

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO beta into TRBC1 HDRT

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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P931_CLYBL-CBX3-Cbh-Zeo-TetO-NAIP2

Plasmid

#202761

Purpose

Encodes four RGC specific transcription factors under the control of a TetOn promoter at the CLYBL safe harbor

Depositor

Karl Wahlin

Article

Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

Insert

NEUROG2-p2A-ATOH7-p2A-ISL1-p2A-POU4F2 (NEUROG2, ATOH7, ISL1, POU4F2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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RAB11A-GFP HDRT Source (pTR 143)

Plasmid

#112012

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-GFP HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 4, 2018

Availability

Academic Institutions and Nonprofits only

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CD4-C-GFP HDRT Source (pTR 152)

Plasmid

#112018

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-GFP HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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CLTA-GFP HDRT Source (pTR 153)

Plasmid

#112016

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CLTA-GFP HDRT (CLTA Human)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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