We narrowed to 8,589 results for: Set

1. pTF-lacZ

   Plasmid

   #153036

   Purpose

   For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. piggyBac CMV-GFP-FRT

   Plasmid

   #92078

   Purpose

   production of transgenic animal and recombination using flipase

   Depositor

   Jae Yong Han

   Article

   Lee et al FASEB J. 2016 Feb;30(2):555-63. doi: 10.

   Insert

   CMV-eGFP and TK-Neo between FRT3 and FRT
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPJ23116-MS2-rep

   Plasmid

   #184842

   Purpose

   Expression of the rep protein of phage MS2

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Replicase gene MS2 phage
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23116

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMCRi

   Plasmid

   #207524

   Purpose

   Plasmid for CRISPRi; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR

   Depositor

   Pablo Ivan Nikel

   Article

   Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

   Insert

   xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR
   

   Use

   Pseudomonas vector

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-CAG-Brainbow3.2-PGK-PuroTK

   Plasmid

   #159928

   Purpose

   Brainbow transgenesis

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Res. 2021 Jan 8;51:102164. doi

   Inserts

   NFPnls
   mOrange2
   EGFP
   mKate2
   

   Expression

   Mammalian

   Available Since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. NylCK_pET28a

   Plasmid

   #215422

   Purpose

   Expression construct for NylCK gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylCK
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. T2A 3xNLS GFP

   Plasmid

   #227721

   Purpose

   Plasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp Resistant

   Depositor

   Oliver Hobert, Haosheng Sun

   Article

   Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

   Insert

   T2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)
   

   Use

   Cloning vector

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. ThcCut1_pET29b

   Plasmid

   #215429

   Purpose

   Expression construct for ThcCut1 gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   ThcCut1
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. UMG-SP-3_pET29b

   Plasmid

   #215435

   Purpose

   Expression construct for UMG-SP-3 gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   UMG-SP-3
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. NfPolyA_pET21b

    Plasmid

    #215413

    Purpose

    Expression construct for NfPolyA gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NfPolyA
    

    Expression

    Bacterial

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. FsC_pET29b

    Plasmid

    #215405

    Purpose

    Expression construct for FsC gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    FsC
    

    Expression

    Bacterial

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. 5HT6-YFP-Inp54p

    Plasmid

    #96812

    Purpose

    cilia-membrane-targeted inositol polyphosphate 5-phosphatase

    Depositor

    Takanari Inoue

    Article

    Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

    Insert

    5HT6-YFP-Inp54p
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbS8c-ddcpf1-rfp

    Plasmid

    #153038

    Purpose

    For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    Ascpf1
    

    Expression

    Bacterial

    Mutation

    E993A

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQdCas9-sgempty

    Plasmid

    #159523

    Purpose

    Expression of dCas9 driven by lux cassette from Vibrio fischeri

    Depositor

    Radhakrishnan Mahadevan

    Article

    Liu et al ACS Synth Biol. 2020 Jun 5. doi: 10.1021

    Insert

    quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA
    

    Use

    CRISPR

    Promoter

    quorum sensing promoter

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNeuroD-ires-GFP

    Plasmid

    #61403

    Purpose

    Mammalian expression vector under the neuron-specific promoter NeuroD. Co-expression of GFP with IRES sequence

    Depositor

    Franck Polleux

    Article

    Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    NeuroD

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFS478

    Plasmid

    #89066

    Purpose

    KanMX Z3EV promoter cassette

    Depositor

    Nick Rhind

    Article

    Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

    Insert

    Z3EV promoter cassette
    

    Use

    Yeast integration vector

    Mutation

    yeast Gal1 promoter modified with multiple Zif268…

    Promoter

    Z3EV promoter

    Available Since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLM-fSV2A

    Plasmid

    #27512

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

    Insert

    OCT4_T2A_KLF4_P2A_cMYC_E2A_Sox2
    

    Use

    Lentiviral

    Available Since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. 5HT6-YFP-PIPK

    Plasmid

    #96810

    Purpose

    cilia-membrane-targeted phosphatidylinositol 4-phosphate 5-kinase

    Depositor

    Takanari Inoue

    Article

    Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

    Insert

    5HT6-YFP-PIPK
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBbS8c-ddcpf1-Δ

    Plasmid

    #153039

    Purpose

    For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    Ascpf1
    

    Expression

    Bacterial

    Mutation

    E993A

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDD283

    Plasmid

    #66824

    Purpose

    YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    YPET-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized YPET

    Expression

    Worm

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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