We narrowed to 13,713 results for: cas9

1. pPW3754 : pUC-SpCas9_gRNA-HsIRE1-Cterm

   Plasmid

   #185676

   Purpose

   SpCas9 and gRNA targeting the C-terminus of HsIRE1

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERN1 gRNA (ERN1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

2. pCMV_UdgX-Anc689-UdgX-NG-nCas9-RBMX

   Plasmid

   #163554

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-Anc689-UdgX-NG-nCas9-RBMX
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Promoter

   Available since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2T-CMV-evoCjCas9-BE4max-BlastR

   Plasmid

   #194061

   Purpose

   Mammalian expression of evoCjCas9 CBE4max

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-BE4max
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. A3Bi-ctd-Cas9n-UGI-NLS

   Plasmid

   #109426

   Purpose

   Expresses the C-terminal catalytic domain of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Insertion of an L1 intron into the coding sequenc…

   Promoter

   CMV

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX

   Plasmid

   #163543

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   POLD2-APOBEC1-UdgX-nCas9-UdgX
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Promoter

   Available since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

   Plasmid

   #78607

   Purpose

   A single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2
   

   Use

   AAV and CRISPR

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short; U6

   Available since

   Sept. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)

   Plasmid

   #71829

   Purpose

   Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   BACH2-sgRNA8 (BACH2 S. pyogenes, Synthetic, Human)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; E756A inactiva…

   Promoter

   U6

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCAS9 wt (BB)-2A-GFP targeting human TRIM21

   Plasmid

   #138295

   Purpose

   gRNA for CRISPR/Cas9 knockout of human TRIM21

   Depositor

   Gaudenz Danuser

   Article

   Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

   Insert

   gRNA TRIM21 (TRIM21 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV_hUbC-dSpCas9-2xVP64-T2A-BSD

   Plasmid

   #162333

   Purpose

   Lentiviral expression of dSpCas9-2xVP64 with blasticidin resistance

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   dSpCas9-2xVP64
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

    Plasmid

    #47550

    Purpose

    Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

    Inserts

    Cas9
    ttTi5605 sgRNA
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Worm

    Mutation

    Codon optimized and with synthetic introns for C.…

    Promoter

    U6 and eef-1A.1 (eft-3)

    Available since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLL8 lenti-D10Acas9-3xflag-blast

    Plasmid

    #112134

    Purpose

    lenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE)

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    D10Acas9-3xflag-blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A mutant in Cas9

    Promoter

    EF1a

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. peSpCas9(1.1)-2×sgRNA (IFT88, donor)

    Plasmid

    #80769

    Purpose

    Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

    Insert

    IFT88 gRNA#1 (IFT88 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. PET-eSpCas9-plus-NLS-6xHis

    Plasmid

    #207380

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K848A, R1060A, amino acids 1005-1013 replaced wit…

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid

    #74408

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A S. pyogenes, Synthetic, Human)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHACK(Gal4)-DONR(T2A-Cas9)

    Plasmid

    #194768

    Purpose

    To insert T2A-Cas9 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Cas9 transgene.

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Insert

    T2A-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pdCas9+sgRNA expression vector precursor

    Plasmid

    #171798

    Purpose

    PICASSO: Library expression vector precursor for paired dCas9+sgRNA expression

    Depositor

    Stephen Elledge

    Article

    Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO

    Available since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CMV-FLEX-SaCas9-U6-sgNts

    Plasmid

    #159909

    Purpose

    Mutagenesis of Nts

    Depositor

    Larry Zweifel

    Insert

    Nts (Nts Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. p2T-cmv-SpCas9-TadDE-BlastR

    Plasmid

    #193850

    Purpose

    Express TadDE in mammalian cells with BlastR selection, Tol2 integration

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadDE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-E1347A-nCas9(D10A)-UGI

    Plasmid

    #187410

    Purpose

    DddA E1347A variant

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA E1347A variant
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-pMecp2-SaCas9-pU6-sgRNA

    Plasmid

    #159173

    Purpose

    Vector A encodes pAAV-pMecp2-SaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of SaCas9-sgRNA in neurons

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pMecp2

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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