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We narrowed to 14,200 results for: cas9

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Showing: 2281 - 2300 of 14200 results

  3. SIN-cPPT-G1B3-spCas9-WPRE-miR124T

    Plasmid
    #245071
    Purpose
    CRISPR Editing with SpCas9, with miRNA-124 target sequence to repress transgene expression in neurons
    Depositor
    Nicole Déglon
    Insert
    Cas9, miR124T site
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Human G1B3 promoter
    Available since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245071-plasmid-map-sequence-id-495712

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  10. pD10AH840AhCas9 (GB1041)

    Plasmid
    #68207
    Purpose
    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part
    Depositor
    Diego Orzaez
    Publication
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed; human codon optimis…
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68207-plasmid-map-sequence-id-135575

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  14. pPC1000

    Plasmid
    #174829
    Purpose
    Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Inserts
    CRISPRi SpCas9 sgRNA cassette
    PuroR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    EF1a and mouse U6
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174829-plasmid-map-sequence-id-343648

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  15. pUC19-E6MBW6

    Plasmid
    #103102
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    E6MBW6(Cas9 coding gene from Staphylococcus lugdunensis M23590)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    103102-plasmid-map-sequence-id-209054

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  17. PB-PE SpRY

    Plasmid
    #179082
    Purpose
    NOTE: An improved version of this plasmid is now available. See Addgene plasmid #235998. All-in-one prime editor piggyBac transposon, SpRY variant
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    A61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…
    Promoter
    CAG
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179082-plasmid-map-sequence-id-352656

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Showing: 2281 - 2300 of 14200 results