We narrowed to 3,563 results for: luciferase expression

1. pPRIME-TREX-GFP-FF3

   Plasmid

   #11667

   Purpose

   3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a Tet-responsive promoter (TREX) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Insert

   FF3
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. bdSUMO-R9-GFP-HiBit

   Plasmid

   #170990

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-R9-muGFP-HiBiT
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. bdSUMO-pHD118-GFP-HiBit

   Plasmid

   #171015

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with pHD118 peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-pHD118-muGFP-HiBiT
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a Gx-NL2

   Plasmid

   #169107

   Purpose

   Expresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

   Depositor

   Maarten Merkx

   Article

   Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

   Insert

   protein G - NanoLuc - NanoLuc
   

   Use

   Tags

   his-tag and strep-tag

   Expression

   Bacterial

   Mutation

   Ala 24 stop

   Promoter

   T7

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCheck2 Dest (R1-R2) #BGV129

   Plasmid

   #48955

   Purpose

   Destination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors.

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Type

   Empty backbone

   Use

   Luciferase; Gateway cloning vector

   Tags

   Expression

   Mutation

   Promoter

   SV40

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pTS2311-Tier1-spacer-PPGK-NLuc_TB

   Plasmid

   #169552

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-NanoLuc Luciferase - mTagBFP fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-IgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc

   Plasmid

   #125229

   Purpose

   Expresses transmembrane intracellular NanoLuc in mammalian cells

   Depositor

   Alice Ting

   Article

   Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

   Insert

   IgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEN_hU6miR-Luc-A

   Plasmid

   #25756

   Purpose

   Entry vector with human U6 promoter driving control Luciferase miR30-based shRNA.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   Luciferase miR-shRNA
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti X2 Puro/pTER shLUC (w589-1)

   Plasmid

   #21472

   Purpose

   3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Puro

   Depositor

   Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   Firefly luciferase shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. bdSUMO-pHlip-GFP-HiBit

    Plasmid

    #171016

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with pHlip peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-pHlip-muGFP-HiBiT
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOPTXcGMPRELUC

    Plasmid

    #68503

    Purpose

    cyclic nucleotide reporter for bacteria or plant cells

    Depositor

    Helen Irving

    Article

    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

    Insert

    OPTX promoter with cGMPRE (AT1G33440 Mustard Weed)
    

    Use

    Tags

    luciferase

    Expression

    Bacterial and Plant

    Mutation

    3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…

    Promoter

    OPTX promoter with cGMPRE

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pLenti X2 Neo/pTER shLUC (w181-1)

    Plasmid

    #17483

    Purpose

    3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Neo 

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti X2 Blast/pTER shLUC (w618-1)

    Plasmid

    #20962

    Purpose

    3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Blast 

    Depositor

    Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly luciferase shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti X2 Zeo/pTER shLUC (w648-1)

    Plasmid

    #21561

    Purpose

    3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Zeo

    Depositor

    Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly luciferase shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. -962 human cyclin D1 promoter TCF (1)-(3) sites mutant pGL3Basic

    Plasmid

    #32735

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 Promoter (CCND1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    -75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…

    Promoter

    -962 CCND1

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pOPTXGARE

    Plasmid

    #68542

    Purpose

    cyclic nucleotide reporter for bacteria or plant cells

    Depositor

    Helen Irving

    Article

    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

    Insert

    OPTX promoter with GARE (AT1G33440 Mustard Weed)
    

    Use

    Tags

    luciferase

    Expression

    Bacterial and Plant

    Mutation

    5x GARE inserted 190 bp 5' pf ATG (GARE = ta…

    Promoter

    OPTX promoter with GARE

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. T7-LacO-Halo-PILATeS-RGD (200nM)

    Plasmid

    #216716

    Purpose

    Expresses HaloTag-PILATeS-RGD with 200 nM BiT tag in bacteria

    Depositor

    Alex Dunn

    Article

    Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

    Insert

    HaloTag-PILATeS-RGD
    

    Use

    Tags

    6xHis and HaloTag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. T7-LacO-Halo-PILATeS-RGD (700pM)

    Plasmid

    #216714

    Purpose

    Expresses HaloTag-PILATeS-RGD with 700 pM BiT tag in bacteria

    Depositor

    Alex Dunn

    Article

    Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

    Insert

    HaloTag-PILATeS-RGD
    

    Use

    Tags

    6xHis and HaloTag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQC.Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)]-Fluc

    Plasmid

    #200124

    Purpose

    Retroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] protease reporter and P2A firefly luciferase in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] P2A firefly luciferase
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQC.Flip-nluc(LgBiT1-8)CP[CoVA]-Fluc

    Plasmid

    #200125

    Purpose

    Retroviral expression of Flip-nluc(LgBiT1-8)CP[CoVA] protease reporter and P2A firefly luciferase in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    Flip-nluc(LgBiT1-8)CP[CoVA] P2A firefly luciferase
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge