Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 17,757 results for: puro

Showing: 4601 - 4640 of 17757 results

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Plasmid

#127665

Purpose

Encodes for puromycin resistance expressed from the EF1a promoter

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

Puro

Expression

Mammalian

Promoter

EF1a

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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sgRNA4_MYOD1

Plasmid

#64139

Purpose

Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_MYOD1 (MYOD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pDECKO_Malat1_E

Plasmid

#72624

Purpose

Expresses two gRNAs targeting MALAT1 promoter

Depositor

Roderic Guigo, Rory Johnson

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward Malat1

Expression

Mammalian

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLib6.6B

Plasmid

#176666

Purpose

Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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p-miR-148a

Plasmid

#65052

Purpose

Expression of miR148a in mammalian cells

Depositor

Cristina Fillat

Article

Bofill-De Ros et al Mol Ther. 2014 Sep;22(9):1665-77. doi: 1

Insert

hsa-miR-148a (pre-miRNA) (MIR148A Human)

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-WRC-vpr

Plasmid

#115839

Purpose

Encodes codon optimized 3xFLAG-SIVWRC Vpr (AM713177) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVWRC Vpr (AM713177)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-AGM-VERAGM3 vpr

Plasmid

#115826

Purpose

Encodes codon optimized 3xFLAG-SIVAGM Vpr (Ver AGM3) (M30931) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVAGM Vpr (Ver AGM3) (M30931)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS-SIV-SAB-92018 vpr

Plasmid

#115822

Purpose

Encodes codon optimized 3xFLAG-SIVSAB Vpr (HQ378594) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVSAB Vpr (HQ378594)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-AGM-TAN1 vpr

Plasmid

#115819

Purpose

Encodes codon optimized 3xFLAG-SIVAGM Vpr (TAN1) (U58991) with repaired premature stop codon and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVAGM Vpr (TAN1)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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sgRNA3_MYOD1

Plasmid

#64138

Purpose

Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_MYOD1 (MYOD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Cquin_596

Plasmid

#176669

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE HSF Flag Ac

Plasmid

#1949

Depositor

Robert Kingston

Article

Sullivan et al Mol Cell Biol 2001 Sep;21(17):5826-37.

Insert

Heat Shock Factor 1 (HSF1 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

Acidic mutations (D416, E493, E496 A)

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pPKm-145

Plasmid

#90505

Purpose

Empty plasmid, pSIN-EF1-alpha-IRES-puro

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Type

Empty backbone

Use

Lentiviral

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-MUS2-1246-vpr

Plasmid

#115841

Purpose

Encodes codon optimized 3xFLAG-SIVMUS21246 Vpr (EF070329) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMUS21246 Vpr (EF070329)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS-SIV-GRV vpr

Plasmid

#115827

Purpose

Encodes codon optimized 3xFLAG-SIVGRV Vpr (M66437) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVGRV Vpr (M66437)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS-SIV-GSN-CN71 vpr

Plasmid

#115830

Purpose

Encodes codon optimized 3xFLAG SIVGSN-CN71 Vpr (AF468658) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVGSN-CN71 Vpr (AF468658)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS-SIV-DEN vpr

Plasmid

#115829

Purpose

Encodes codon optimized 3xFLAG SIVDEN Vpr (AJ580407) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVDEN Vpr (AJ580407)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS-SIV-AGM-MAL vpr

Plasmid

#115828

Purpose

Encodes codon optimized 3xFLAG-SIVAGM Vpr (MAL_ZMB) (LC114462) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVAGM Vpr (MAL_ZMB) (LC114462)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS-SIV-ASC vpr

Plasmid

#115824

Purpose

Encodes codon optimized 3xFLAG-SIVASC Vpr (KJ461715) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVASC Vpr (KJ461715)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS-SIV-DEB vpr

Plasmid

#115823

Purpose

Encodes codon optimized 3xFLAG-SIVDEB Vpr (FJ919724) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVDEB Vpr (FJ919724)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS-SIV-LST vpr

Plasmid

#115818

Purpose

Encodes codon optimized 3xFLAG-SIVLST Vpr (AF188116) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVLST Vpr (AF188116)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS-SIV-MND1 vpr

Plasmid

#115817

Purpose

Encodes codon optimized 3xFLAG-SIVMND1 Vpr (M27470) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMND1 Vpr (M27470)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4-Aalb_726

Plasmid

#176661

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4-Aaeg_763

Plasmid

#176658

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUPI

Plasmid

#80279

Purpose

A bicistronic lentiviral vector used to stably express cDNAs.

Depositor

Jeremy Luban

Article

Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

Insert

Puro resistance cassette, IRES, cloning site

Use

Lentiviral

Expression

Mammalian

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS-SIV-COL-BWC01 vpr

Plasmid

#115840

Purpose

Encodes codon optimized 3xFLAG-SIVCOLVpr (KF214240) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVCOLVpr (KF214240)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS-SIV-RCM-vpr

Plasmid

#115838

Purpose

Encodes codon optimized 3xFLAG-SIVRCM Vpr (HM803689) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVRCM Vpr (HM803689)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS-SIV-SUN-K08 vpr

Plasmid

#115832

Purpose

Encodes codon optimized 3xFLAG-SIVSUN Vpr (FR751162) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVSUN Vpr (FR751162)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS-SIV-GSN-CN166 vpr

Plasmid

#115831

Purpose

Encodes codon optimized 3xFLAG SIVGSN-CN166 Vpr (AF468659) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVGSN-CN166 Vpr (AF468659)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS-SIV-SYK vpr

Plasmid

#115821

Purpose

Encodes codon optimized 3xFLAG-SIVSYK Vpr (L06042) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVSYK Vpr (L06042)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS-SIV-TAL vpr

Plasmid

#115820

Purpose

Encodes codon optimized 3xFLAG-SIVTAL Vpr (AM182197) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVTAL Vpr (AM182197)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS-SIV-AGI vpx

Plasmid

#115815

Purpose

Encodes codon optimized 3xFLAG-SIVAGI Vpx (HM803690) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVAGI Vpx (HM803690)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS-SIV-DRL vpx

Plasmid

#115814

Purpose

Encodes codon optimized 3xFLAG-SIVDRL Vpx (KM378563) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVDRL Vpx (KM378563)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS-SIV-MNE vpx

Plasmid

#115813

Purpose

Encodes codon optimized 3xFLAG-SIVMNE Vpx (U79412) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMNE Vpx (U79412)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-mPar6a WT

Plasmid

#86148

Purpose

FLAG tagged mouse Par6 wild type - Binds to aPKC, Par3, Lgl, Cdc42

Depositor

Senthil Muthuswamy

Article

Nolan et al Cancer Res. 2008 Oct 15;68(20):8201-9.

Insert

Par6 (Pard6a Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

LTR

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only
ARL13B-(C8C9- SS)-GFP

Plasmid

#246842

Purpose

Express Arl13B (resistance to sgRNA), tagged with GFP, with C8C9-SS mutation

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

changed Cysteine 8 and 9 to Serine

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
ARL13B-GFP(1-245)

Plasmid

#246847

Purpose

Express Arl13B (resistance to sgRNA), aa1-245, tagged with GFP

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 246-428

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-CMV-CNOT3-E95A-Tev-3xFlag

Plasmid

#245726

Purpose

Express CNOT3-E95A-Tev-3xFlag in mammalian cells

Depositor

Joshua Mendell

Article

Zhu et al Science. 2024 Nov 22;386(6724):eadq8587.

Insert

CNOT3-E95A-Tev-3xFlag (CNOT3 Human)

Use

Lentiviral

Tags

3xFlag

Mutation

E95A

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-CMV-CNOT3-Tev-3xFlag

Plasmid

#245724

Purpose

Express CNOT3-Tev-3xFlag in mammalian cells

Depositor

Joshua Mendell

Article

Zhu et al Science. 2024 Nov 22;386(6724):eadq8587.

Insert

CNOT3-Tev-3xFlag (CNOT3 Human)

Use

Lentiviral

Tags

3xFlag

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-CMV-CNOT3-K105S-Tev-3xFlag

Plasmid

#245727

Purpose

Express CNOT3-K105S-Tev-3xFlag in mammalian cells

Depositor

Joshua Mendell

Article

Zhu et al Science. 2024 Nov 22;386(6724):eadq8587.

Insert

CNOT3-K105S-Tev-3xFlag (CNOT3 Human)

Use

Lentiviral

Tags

3xFlag

Mutation

K105S

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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