Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 4,420 results for: DUR

Showing: 2301 - 2320 of 4420 results

  4. pLCHKO_intergenic-intergenic_2

    Plasmid
    #155078
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155078-plasmid-map-sequence-id-308534

    Enlarge

  10. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #86613
    Purpose
    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Tags
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86613-plasmid-map-sequence-id-168851

    Enlarge

  13. p5E-Runx1+23

    Plasmid
    #69602
    Purpose
    Runx1 +23 kb intronic enhancer from mouse and beta-globin minimal promoter in a 5' entry vector for gateway cloning
    Depositor
    Owen Tamplin, Leonard Zon
    Article
    Tamplin et al Cell. 2015 Jan 15;160(1-2):241-52. doi:
    Inserts
    Runx1 +23 intronic enhancer (Runx1 Mouse)
    Mouse beta-globin minimal promoter
    Use
    5' gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    C57/BL6 mouse Runx1 +23 intronic enhancer and C57…
    Available since
    Sept. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    69602-plasmid-map-sequence-id-190603

    Enlarge

  14. pCAGGS-RaichuEV-Rac1(Y40C)

    Plasmid
    #164903
    Purpose
    Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation Y40C in the Rac1 domain. More useful than T17N mutation due to less harmful to endogenous signaling.
    Depositor
    Takeshi Imai
    Article
    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi
    Insert
    RaichuEV-Rac1 (RAC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Y40C mutation in the Rac1 domain whcih make the s…
    Promoter
    Available since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    164903-plasmid-map-sequence-id-340567

    Enlarge

  15. ATP1A1_plasmid_donor_RD

    Plasmid
    #86551
    Purpose
    Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 (ATP1A1 Human)
    Use
    CRISPR and TALEN; Co-selection via hdr using ouab…
    Tags
    Expression
    Mammalian
    Mutation
    Q118R, N129D
    Promoter
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86551-plasmid-map-sequence-id-171421

    Enlarge

  16. pTon1(il11)

    Plasmid
    #233647
    Purpose
    Expression vector of X. laevis il11.L-P2A-AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.
    Depositor
    Takeo Kubo
    Article
    Tsujioka et al Nat Commun. 2017 Sep 8;8(1):495. doi: 10
    Inserts
    interleukin-11.L (350-913) (il11.L Frog)
    target sequence of guide RNA #tyr (3905-3927) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
    Use
    Amphibian expression, tet-on
    Tags
    Expression
    Mutation
    Some synonymous substitutions were introduced in…
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233647-plasmid-map-sequence-id-468199

    Enlarge

  17. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

    Plasmid
    #86612
    Purpose
    Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Tags
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86612-plasmid-map-sequence-id-168849

    Enlarge

Showing: 2301 - 2320 of 4420 results