We narrowed to 2,467 results for: PUC19

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HBT-CPK32ac-Flag

Plasmid

#107431

Purpose

For Arabidopsis mesophyll protoplast transient expression assay

Depositor

Jen Sheen

Article

Liu et al Nature. 2017 May 18;545(7654):311-316. d

Insert

CPK32 (CPK32 Mustard Weed)

Tags

Flag

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pZNF134-donor

Plasmid

#112320

Purpose

CRISPR donor plasmid to tag human transcription factor ZNF134 with GFP

Depositor

Kevin White

Insert

ZNF134 homology arms flanking EGFP-IRES-Neo cassette (ZNF134 Human)

Use

CRISPR

Mutation

hg38:19:57621700:C>T

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pGLIS3-donor

Plasmid

#113812

Purpose

CRISPR donor plasmid to tag human transcription factor GLIS3 with GFP

Depositor

Kevin White

Insert

GLIS3 homology arms flanking EGFP-IRES-Neo cassette (GLIS3 Human)

Use

CRISPR

Mutation

rs10118031, rs10973702, rs912257

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pBCL6-donor

Plasmid

#112389

Purpose

CRISPR donor plasmid to tag human transcription factor BCL6 with GFP

Depositor

Kevin White

Insert

BCL6 homology arms flanking EGFP-IRES-Neo cassette (BCL6 Human)

Use

CRISPR

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pZNF140-donor

Plasmid

#112363

Purpose

CRISPR donor plasmid to tag human transcription factor ZNF140 with GFP

Depositor

Kevin White

Insert

ZNF140 homology arms flanking EGFP-IRES-Neo cassette (ZNF140 Human)

Use

CRISPR

Mutation

hg38:12:133105834:A>G, hg38:12:133105852:A>G

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pSRF-donor

Plasmid

#112362

Purpose

CRISPR donor plasmid to tag human transcription factor SRF with GFP

Depositor

Kevin White

Insert

SRF homology arms flanking EGFP-IRES-Neo cassette (SRF Human)

Use

CRISPR

Mutation

hg38:6:43178470:C>A, hg38:6:43178514:G>A

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pNR2F6-donor

Plasmid

#112360

Purpose

CRISPR donor plasmid to tag human transcription factor NR2F6 with GFP

Depositor

Kevin White

Insert

NR2F6 homology arms flanking EGFP-IRES-Neo cassette (NR2F6 Human)

Use

CRISPR

Mutation

hg38:19:17232898:A>G

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pZNF584-donor

Plasmid

#112342

Purpose

CRISPR donor plasmid to tag human transcription factor ZNF584 with GFP

Depositor

Kevin White

Insert

ZNF584 homology arms flanking EGFP-IRES-Neo cassette (ZNF584 Human)

Use

CRISPR

Mutation

hg38:19:58417078:T>C, rs7257872, rs3764535

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pZNF507-donor

Plasmid

#112305

Purpose

CRISPR donor plasmid to tag human transcription factor ZNF507 with GFP

Depositor

Kevin White

Insert

ZNF507 homology arms flanking EGFP-IRES-Neo cassette (ZNF507 Human)

Use

CRISPR

Mutation

rs2270399, hg38:19:32382978:A>T, rs2111503

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pPN278

Plasmid

#91653

Purpose

Express sgRNA targeting human PTN

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

PTN (PTN Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN279

Plasmid

#91654

Purpose

Express sgRNA targeting human PTN

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

PTN (PTN Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCBS-5738

Plasmid

#249324

Purpose

To express the EPEC DarT2(DLAA)-nScCas9(D10A) fusion protein in experiments for Append Editing

Depositor

Chase Beisel

Article

Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038

Insert

EPEC DarT2(DLAA)-nScCas9(D10A)

Expression

Yeast

Mutation

DarT2(G49D, M86L, R92A, R193A) and nScCas9(D10A)

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pCBS-5753

Plasmid

#249325

Purpose

To express the EPEC dDarT2(DLAA)-nScCas9(D10A) fusion protein in experiments for Append Editing

Depositor

Chase Beisel

Article

Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038

Insert

EPEC dDarT2(DLAA)-nScCas9(D10A)

Expression

Yeast

Mutation

DarT2(G49D, M86L, R92A, E,170A, R193A) and nScCas…

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pMT1375

Plasmid

#228314

Purpose

Constitutive expression of mutant rTetTA

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

rtetTA mutant

Use

Synthetic Biology

Tags

Three copies of VP16 (VP48)

Expression

Yeast

Mutation

K8N

Promoter

KpGAPDH promoter

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Solo-mStayGold-MAP4

Plasmid

#227296

Purpose

Donor template for mStayGold insertion into the N-terminus of the MAP4 locus. For microtubule visualization. To be co-transfected with sgRNAplasmid px330-PITCh-MAP4 (Addgene #227295)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

MAP4 Homology Arms flanking a mStayGold Tag (MAP4 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-Solo-mStayGold-CEP192

Plasmid

#227289

Purpose

Donor template for mStayGold insertion into the C-terminus of the CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

CEP192 Homology Arms flanking a mStayGold Tag (CEP192 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBV90

Plasmid

#204601

Purpose

Expresses OsTir1-3X Myc from S. cerevisiae ADH1 promoter

Depositor

Scott Moye-Rowley

Insert

Os Tir1 (LOC4338955 Oryza sativa)

Tags

Myc epitope tags

Expression

Bacterial

Promoter

ScADH1

Available Since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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pJQR3

Plasmid

#218289

Purpose

Introduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6

Depositor

Claudia Vickers

Article

McDonnell et al bioRxiv 2024.03.26.586389

Insert

HO(-253,-1)-pADH1>LmrA>MIG1(aa123-504)>tMIG1-tYGL036W

Expression

Yeast

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pToxAmp12

Plasmid

#218286

Purpose

Multi-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modules

Depositor

Claudia Vickers

Article

Evans et al bioRxiv 2024.01.30.578080

Insert

HO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)

Expression

Yeast

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pToxAmp1

Plasmid

#218285

Purpose

Amplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanism

Depositor

Claudia Vickers

Article

Evans et al bioRxiv 2024.01.30.578080

Insert

HO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)

Expression

Yeast

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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