We narrowed to 13,211 results for: BASE
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Plasmid#123335PurposeCyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.DepositorInsertCerulean3-Cerulean3-FLARE-AKAR
Tags6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…ExpressionMammalianPromoterCMVAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEU3-NII-HxHLICNot
Plasmid#140183PurposeUsed for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 12xHis-tag and NotI restriction siteDepositorTypeEmpty backboneUseExpression vector for a cell-free systemTags12xHisPromoterT7Available SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEU3-NII-GLICNot-C-BIOT
Plasmid#140186PurposeUsed for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, GST-tag and biotinylation site, and NotI restriction siteDepositorTypeEmpty backboneUseExpression vector for a cell-free systemTagsBiotinylation and GSTPromoterT7Available SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRcCMV-STAT31F
Plasmid#134725PurposeExpresses chimeric STAT3-STAT1 protein with human STAT3 amino acids 1-514 joined to human STAT1 at amino acid 509, with a FLAG epitope tag at the C-terminus. Based on addgene plasmid #8691 and #8707DepositorTagsFLAG and human STAT3 aa 1-514 joined to human STA…ExpressionMammalianAvailable SinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRcCMV-STAT13F
Plasmid#134723PurposeExpresses chimeric STAT1-STAT3 protein with human STAT1 amino acids 1-508 joined to human STAT3 at amino acid 515, with a FLAG epitope tag at the C-terminus. Based on addgene plasmids #8691 and #8707DepositorTagsFLAG and human STAT1 aa1-508 joined to human STAT…ExpressionMammalianAvailable SinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-gR-PnGW
Plasmid#133355PurposePiggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications), co-expressed with GFP-NLSDepositorInsertGFP-NLS
TagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAav-IQGAP1-PQS1-3xFLAG
Plasmid#84885PurposerAAV-based template for genome engineering of the IQGAP1 C-terminus containing PQS1 and 3xFLAG tags and a selection cassetteDepositorInsertsUseAAVTagsPQS1 3xFLAGPromoternoAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAav-TP53-PQS1-3xFLAG
Plasmid#84884PurposerAAV-based template for genome engineering of the p53 C-terminus containing PQS1 and 3xFLAG tags and a selection cassetteDepositorInsertsUseAAVTagsPQS1 3xFLAGPromoterno and noAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA, Pbl-A DH domain
Plasmid#69794PurposePebble isoform A, DH domain, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble DH domain
UseP element-based puast vector for gal4-regulated e…TagsHA-TagExpressionInsectMutationContains only the Pebble DH domainPromoterhsp70Available SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA, Pbl-A C-Term
Plasmid#69795PurposePebble isoform A, C-Term, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble C-Term domain (Drosophila guanine nucleotide exchange factor)
UseP element-based puast vector for gal4-regulated e…TagsHA-TagExpressionInsectMutationJust the C-Term domain of Pebble (pbl)Promoterhsp70 promoterAvailable SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only