163,672 results

1. BPK3082

   Plasmid

   #78742

   Purpose

   Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   LbCpf1 crRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. RBD-Fc

   Plasmid

   #164221

   Purpose

   Expresses soluble , dimeric Fc-His fusion protein for the original SARS-CoV2 Spike RBD region, along with dTomato reporter

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

   Insert

   Spike RBD (S SARS-CoV)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZac2.1 gfaABC1D-BioID2-BioID2-HA

   Plasmid

   #176740

   Purpose

   Expresses the promiscuous small biotin ligase, BioID2, in astrocytes

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   BioID2-BioID2-HA
   

   Use

   AAV

   Tags

   HA

   Expression

   Bacterial

   Mutation

   Promoter

   gfaABC1D

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pICSL60004

   Plasmid

   #117519

   Purpose

   rbcS-E9 terminator Golden Gate Level 0

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BsaI:GCTT:E9t:CGCT:BsaI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p35S:Citrine-TurboID-3xHA

   Plasmid

   #199243

   Purpose

   This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

   Insert

   Citrine-TurboID
   

   Use

   Tags

   3xHA and TurboID-3xHA

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available Since

   June 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGem-PgpdA-Cre-TtrpC

   Plasmid

   #168784

   Purpose

   Helper vector for the transient expression of Cre recombinase in filamentous fungi. Not replicative in fungi.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102

   Insert

   Cre
   

   Use

   Cre/Lox and Synthetic Biology; Expression in fila…

   Tags

   Expression

   Mutation

   Promoter

   PgpdA

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-PGK-Neo-PIP-NLS-mVenus

   Plasmid

   #118619

   Purpose

   Lentiviral expression of the cell cycle marker PIP-NLS-mVenus

   Depositor

   Jean Cook

   Article

   Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

   Insert

   PIP-NLS-mVenus
   

   Use

   Lentiviral

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-RhoA

   Plasmid

   #23224

   Depositor

   Channing Der

   Article

   Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

   Insert

   RhoA (RHOA Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 24, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pJH2971

   Plasmid

   #100955

   Purpose

   Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX marker

   Depositor

   James Haber

   Article

   Ranjith et al Protocol Exchange (2017)

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCasso

    Plasmid

    #207530

    Purpose

    Conditionally-replicating in Pseudomonas plasmid for cytidine base editing based on pS44i8GH-2; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sg

    Depositor

    Pablo Ivan Nikel

    Article

    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

    Insert

    cytidine base editing; oriV(pRO1600/ColE1), xylS, Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sgRNA
    

    Use

    CRISPR; Pseudomonas vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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