We narrowed to 6,011 results for: pcDNA 3.1

1. pcDNA3.1/hFGFR3-K650M-V5

   Plasmid

   #214909

   Purpose

   expression of the K650M mutant variant of human FGFR3, which is associated with Severe Achondroplasia with Developmental Delay and Acanthosis Nigricans (SADDAN)

   Depositor

   Pavel Krejčí

   Article

   Kunova Bosakova et al Proc Natl Acad Sci U S A. 2019 Feb 19. p

   Insert

   FGFR3 (FGFR3 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   K650M substitution

   Promoter

   CMV

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-mGCC2-FLAG

   Plasmid

   #214166

   Purpose

   Transient expression of GCC2

   Depositor

   Nan Yan

   Article

   Tu et al Nat Commun. 2022 Nov 15;13(1):6977. doi:

   Insert

   GCC2 (Gcc2 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-VcnTS-E1015A

   Plasmid

   #213415

   Purpose

   Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).

   Depositor

   Brenton Hoffman

   Article

   Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

   Insert

   VcnTS-E1015A (VCL Chicken, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mutated vinculin glutamic acid 1015 to alanine (E…

   Promoter

   CMV

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-VcnTS-E1021A

   Plasmid

   #213416

   Purpose

   Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).

   Depositor

   Brenton Hoffman

   Article

   Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

   Insert

   VcnTS-E1021A (VCL Chicken, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mutated vinculin glutamic acid 1021 to alanine (E…

   Promoter

   CMV

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-VcnCS-E1015A

   Plasmid

   #213412

   Purpose

   Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).

   Depositor

   Brenton Hoffman

   Article

   Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

   Insert

   VcnCS-E1015A (VCL Chicken, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mutated vinculin glutamic acid 1015 to alanine (E…

   Promoter

   CMV

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-VcnCS-E1021A

   Plasmid

   #213413

   Purpose

   Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).

   Depositor

   Brenton Hoffman

   Article

   Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

   Insert

   VcnCS-E1021A (VCL Chicken, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mutated vinculin glutamic acid 1021 to alanine (E…

   Promoter

   CMV

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)GαoA-91-NLuc

   Plasmid

   #207151

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAO
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position L(91)G

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)GαoB-91-NLuc

   Plasmid

   #207152

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAO
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position L(91)G

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. GpACR1-pcDNA3.1-mScarlet

   Plasmid

   #204964

   Purpose

   Expression of GpACR1 in fusion with mScarlet in mammalian cells

   Depositor

   Peter Hegemann

   Article

   Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

   Insert

   GpACR1
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (+ enhancer)

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1+FLAG-KrasnatQ61R

    Plasmid

    #206843

    Purpose

    To express mouse Kras encoded by native mouse codons and with a Q61R mutation with an N-terminal FLAG tag.

    Depositor

    Christopher Counter

    Article

    Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

    Insert

    Kras (Kras Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Glutamine 61 to Arginine

    Promoter

    Available since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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