We narrowed to 7,507 results for: PAC;

1. pFGC5941-PacI-ath-STTM170/171

   Plasmid

   #84097

   Purpose

   target miRNA170/171 for destruction in Arabidopsis thaliana

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Insert

   STTM170/171
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   2x35S

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC-EsaR-I70V/D91G/V220A

   Plasmid

   #47647

   Purpose

   pAC-EsaR with a208g, a272g, c659t mutations in esaR

   Depositor

   Cynthia Collins

   Article

   Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

   Insert

   esaR-I70V/D91G/V220A
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Changed Isoleucine 70 to Valine, Aspartic acid 91…

   Promoter

   Plac

   Available since

   Sept. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1-Cerulean-Epac2(R53A)-Venus

   Plasmid

   #138197

   Purpose

   FRET-based Epac2 activation sensor with R53A mutation in Epac2 coding sequence.

   Depositor

   Jin Zhang

   Article

   Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

   Insert

   Cerulean-Epac2(R53A)-Venus (Rapgef4 Mouse, Synthetic)
   

   Use

   Tags

   Cerulean and Venus

   Expression

   Mammalian

   Mutation

   Contains arginine-to-alanine substitution at resi…

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-Cerulean-Epac2(R466A)-Venus

   Plasmid

   #138199

   Purpose

   FRET-based Epac2 activation sensor with R466A mutation in Epac2 coding sequence.

   Depositor

   Jin Zhang

   Article

   Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

   Insert

   Cerulean-Epac2(R466A)-Venus (Rapgef4 Mouse, Synthetic)
   

   Use

   Tags

   Cerulean and Venus

   Expression

   Mammalian

   Mutation

   Contains arginine-to-alanine substitution at resi…

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-Cerulean-Epac2(L426W)-Venus

   Plasmid

   #138200

   Purpose

   FRET-based Epac2 activation sensor with L426W mutation in Epac2 coding sequence.

   Depositor

   Jin Zhang

   Article

   Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

   Insert

   Cerulean-Epac2(L426W)-Venus (Rapgef4 Mouse, Synthetic)
   

   Use

   Tags

   Cerulean and Venus

   Expression

   Mammalian

   Mutation

   Contains leucine-to-tryptophan substitution at re…

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. LAP2 Full I pAcGFP-N1 monomeric GFP (1317)

   Plasmid

   #62044

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   LAP2beta (Tmpo Rat)
   

   Use

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

   Plasmid

   #126705

   Purpose

   donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

   Insert

   GFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACEBac1-POMP-PSMG1-PSMG2-PSMG3-PSMG4

   Plasmid

   #214137

   Purpose

   Insect cell expression of the human 20SCP proteasome assembly chaperones

   Depositor

   Brenda Schulman

   Article

   Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

   Inserts

   POMP (POMP Human)
   PSMG1 (PSMG1 Human)
   PSMG2 (PSMG2 Human)
   PSMG3 (PSMG3 Human)
   PSMG4 (PSMG4 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFGC5941-PacI-ath-STTM165/166-48nt

   Plasmid

   #84091

   Purpose

   target miRNA165/166 for destruction in Arabidopsis thaliana

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Insert

   STTM165/166
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   2x35S

   Available since

   Nov. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-CMV-Pink Flamindo-p2a-bPAC

    Plasmid

    #202716

    Purpose

    Mammalian co-expression of soluble Pink Flamindo, a fluorescent cAMP indicator and soluble bPAC, a photoactivatable adenylyl cyclase

    Depositor

    Adam Cohen

    Article

    Xiang et al bioRxiv 2023

    Inserts

    Pink Flamindo
    bPAC
    

    Use

    Lentiviral

    Tags

    10xHis and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2 LRP6-APEX2-Ires-eGFP-PAC

    Plasmid

    #180142

    Purpose

    used to biotinylate targets that are recruited near the receptor during Wnt signaling at different time periods

    Depositor

    Edward De Robertis

    Article

    Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

    Insert

    Human LRP6 (LRP6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-camk2-Sthk-p2a-bpac(WT) minWPRE

    Plasmid

    #118274

    Purpose

    Expresses the SthK channel & bPAC(WT)-mCherry under a minimal CamKII promoter

    Depositor

    Dietmar Schmitz

    Article

    Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

    Inserts

    bPAC(WT)
    SthK
    

    Use

    AAV

    Tags

    His tag, P2A, mCherry, and myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CamK II

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. attB53-Pac-TRE-3xFlag-Neurog1-attB53

    Plasmid

    #183613

    Purpose

    RMCE vector to shuttle puromycin resistance and TRE-3xFlag-Neurog1 cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    Neurog1 (Neurog1 Mouse)
    

    Use

    Rmce

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pD649-HAsp-SPACA6-COMP5AP-AviTag-9xHis

    Plasmid

    #157274

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SPACA6 (SPACA6 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACEBac1-PSMB5-PSMB6-PSMB7-TEV-2xSTII

    Plasmid

    #217896

    Purpose

    Insect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7) with C-terminal Twin-Strep-tag

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMB5 (PSMB5 Human)
    PSMB6 (PSMB6 Human)
    PSMB7 (PSMB7 Human)
    

    Use

    Tags

    2xSTII

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEM-HE-2xLyn-ERex-Venus-bPAC(F198Y)

    Plasmid

    #165489

    Purpose

    Xenopus oocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

    Depositor

    Thomas Oertner

    Article

    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

    Insert

    2xLyn-ERex-Venus-bPAC(F198Y)
    

    Use

    Cdna expression, xenopus oocyte

    Tags

    Venus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-mEGFP(A206K)-paCaMKII(K42M)-ShadowG

    Plasmid

    #167477

    Purpose

    Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(K42M)-ShadowG
    

    Use

    Tags

    ShadowG and mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CMV-mEGFP(A206K)-paCaMKII(T286A)-ShadowG

    Plasmid

    #167476

    Purpose

    Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(T286A)-ShadowG
    

    Use

    Tags

    ShadowG and mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV hSyn1 bPAC cMyc S27A 2A tDimer

    Plasmid

    #85398

    Purpose

    humanized photoactivated adenylyl cyclase lower dark activity, spectrum shifted 15 nm longer wavelenght with neuron-specific promoter, plus red fluorescent protein

    Depositor

    Thomas Oertner

    Inserts

    humanized photoactivated adenylyl cyclase
    red fluorescent protein
    

    Use

    AAV

    Tags

    cMyc Tag

    Expression

    Mammalian

    Mutation

    S27A

    Promoter

    human Synapsin1 promotor and ribosomal skip seque…

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAC8_PH-GST-Nter-GWs-Lox (VE5586)

    Plasmid

    #163766

    Purpose

    Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter GST tag under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    N-terminal GST tag
    

    Use

    Tags

    GST Tag

    Expression

    Insect

    Mutation

    Promoter

    PH

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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