We narrowed to 2,612 results for: Lox

1. MADR pDonor smFP-HA (dark) WPRE

   Plasmid

   #131007

   Purpose

   MADR donor plasmid for FlpO+Cre- mediated insertion of SM_FP-HA, a cytoplasmic fluorescent protein which includes multiple HA epitope tags

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   smFP-HA WPRE
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   HA - 10 total HA epitope tages

   Expression

   Mammalian

   Mutation

   "dark" variant with GGG fluorphore comp…

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MADR pDonor smFP-V5 (dark) WPRE

   Plasmid

   #131006

   Purpose

   MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-V5, a cytoplasmic fluorescent protein which includes multiple V5 epitope tags

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   smFP-V5 WPRE
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   V5 - 10 total of V5 epitope tag

   Mutation

   "dark" variant with GGG fluorphore comp…

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pyrFEKO-NEO

   Plasmid

   #24022

   Depositor

   George Cross

   Type

   Empty backbone

   Use

   Floxed targeting vector for trypanosome

   Available Since

   May 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. ZM (pyrFEKO-HYG TetR-T7RNAP)

   Plasmid

   #31455

   Depositor

   George Cross

   Insert

   
   

   Use

   Floxed targeting vector for trypanosome

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pMyt1GFPicre-Knock-In

   Plasmid

   #22657

   Depositor

   Lynn Hudson

   Insert

   Myelin Transcription Factor 1 (Myt1 Mouse)
   

   Use

   Targeting vector

   Available Since

   Nov. 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUltra-puro-RTTA3

   Plasmid

   #58750

   Purpose

   silence doxycyclin inducible pUltra-dox

   Depositor

   Kitai Kim, Yildirim Dogan

   Insert

   Reverse TET-Transactivator 3
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   loxP5171

   Promoter

   Ubiquitin C promoter

   Available Since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-DIO-mCherry

   Plasmid

   #50462

   Purpose

   Double floxed mCherry under the control of EF1a promoter

   Depositor

   Bryan Roth

   Insert

   mCherry
   

   Use

   AAV

   Tags

   N/A

   Promoter

   EF1a

   Available Since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.synP.DIO.EGFP.WPRE.hGH

   Plasmid

   #100043

   Purpose

   An AAV vector that expresses EGFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

   Depositor

   Ian Wickersham

   Has Service

   AAV9

   Insert

   EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   synapsin-1

   Available Since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUltra-Smurf-luc-RTTA3

   Plasmid

   #59438

   Purpose

   silence doxycyclin inducible pUltra-dox (also expresses luciferase)

   Depositor

   Kitai Kim, Yildirim Dogan

   Inserts

   luciferase
   Reverse TET-Transactivator 3
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   loxP5171

   Available Since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pK-11

    Plasmid

    #22094

    Depositor

    Gail Martin

    Article

    Meyers et al Nat Genet. 1998 Feb . 18(2):136-41.

    Insert

    Frt-PGKNeo-Frt-LoxP
    

    Use

    Bacterial

    Available Since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

11. 1782_pAAV-U6-Ai9-Sa-gRNA2-CB-SACas9-HA-OLLAS-spA_C

    Plasmid

    #135978

    Purpose

    gRNA against the right loxP site of the Ai9 allele

    Depositor

    William Lagor

    Insert

    Ai9 gRNA2
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    HA

    Promoter

    CB

    Available Since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. 1781_pAAV-U6-Ai9-Sa-gRNA1-CB-SACas9-HA-OLLAS-spA_C

    Plasmid

    #135979

    Purpose

    gRNA against the left loxP site of the Ai9 allele

    Depositor

    William Lagor

    Insert

    Ai9 gRNA1
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    HA

    Promoter

    U6

    Available Since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSA53

    Plasmid

    #35115

    Depositor

    John McCusker

    Article

    Ito-Harashima et al Yeast. 2004 Jan 15;21(1):53-61.

    Insert

    callys5 flanked by loxp
    

    Use

    Yeast replicating

    Available Since

    Aug. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-DIO-mCherry

    Plasmid

    #50459

    Purpose

    Double floxed mCherry under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    mCherry
    

    Use

    AAV

    Tags

    N/A

    Promoter

    human Synapsin 1

    Available Since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-DIO-EGFP

    Plasmid

    #50457

    Purpose

    Double floxed EGFP under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP
    

    Use

    AAV

    Tags

    N/A

    Promoter

    human Synapsin 1

    Available Since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Ef1a-vCreDIO hChR2(H134R)-eYFP 2.0

    Plasmid

    #126081

    Purpose

    vCre-Dependent ChR2(H134R)-eYFP with the vLox sites in the reverse orientation

    Depositor

    Karl Deisseroth

    Insert

    hChR2(H134R)-eYFP
    

    Use

    AAV

    Tags

    eYFP

    Mutation

    H134R

    Promoter

    Ef1a

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Ef1a-sCreDIO hChR2(H134R)-eYFP 2.0

    Plasmid

    #126080

    Purpose

    sCre-dependent ChR2(H134R)-eYFP with the sLox sites in the reverse orientation

    Depositor

    Karl Deisseroth

    Insert

    hChR2(H134R)-eYFP
    

    Use

    AAV

    Tags

    eYFP

    Mutation

    H134R

    Promoter

    Ef1a

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSA40

    Plasmid

    #35114

    Depositor

    John McCusker

    Article

    Ito-Harashima et al Yeast. 2004 Jan 15;21(1):53-61.

    Insert

    lys5 flanked by loxp
    

    Use

    Yeast replicating

    Available Since

    Aug. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-DIO-rM3D(Gs)-mCherry

    Plasmid

    #50458

    Purpose

    Double floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV8

    Insert

    rM3D-mCherry
    

    Use

    AAV

    Tags

    HA and mCherry

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHygR8

    Plasmid

    #200221

    Purpose

    hygR selection nls-mScarlet marked rapid RMCE integration vector without loxP site

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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