We narrowed to 3,452 results for: ttl

1. pJYDN3

   Plasmid

   #162456

   Purpose

   Yeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.

   Depositor

   Gideon Schreiber

   Article

   Zahradník et al bioRxiv Preprint 2020

   Type

   Empty backbone

   Use

   Shuttle vector bacteria/yeast

   Tags

   ALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pESC-URA-GFP-Ubc9wt

   Plasmid

   #20368

   Depositor

   Judith Frydman

   Article

   Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

   Insert

   SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)
   

   Use

   Yeast/bacteria shuttle

   Tags

   GFP

   Expression

   Yeast

   Available Since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

3. FRP2372

   Plasmid

   #127578

   Purpose

   Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Lys2 selection marker

   Depositor

   Joerg Stelling

   Article

   Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

   Insert

   PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
   

   Use

   Synthetic Biology; Shuttle vector

   Expression

   Yeast

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. FRP2374

   Plasmid

   #127579

   Purpose

   Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 selection marker

   Depositor

   Joerg Stelling

   Article

   Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

   Insert

   PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
   

   Use

   Synthetic Biology; Shuttle vector

   Expression

   Yeast

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJYDN2

   Plasmid

   #162454

   Purpose

   Yeast display plasmid intended for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA) and expression of N-terminal fusions with Aga2p yeast agglutinin.

   Depositor

   Gideon Schreiber

   Article

   Zahradník et al bioRxiv Preprint 2020

   Type

   Empty backbone

   Use

   Shuttle vector bacteria/yeast

   Tags

   DnbALFA nanobody and Ha and myc

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJYDN2p

   Plasmid

   #162455

   Purpose

   Positive control plasmid for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA).

   Depositor

   Gideon Schreiber

   Article

   Zahradník et al bioRxiv Preprint 2020

   Insert

   Aga2p-DnbALFA (AGA2 )
   

   Use

   Shuttle vector bacteria/yeast

   Tags

   DnbALFA nanobody and HA and myc

   Promoter

   GAL1

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pESC-URA-Ub-G76A-Rnq1-GFP

   Plasmid

   #21052

   Depositor

   Judith Frydman

   Article

   Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

   Insert

   Rnq1 (RNQ1 Budding Yeast)
   

   Use

   Yeast/bacteria shuttle

   Tags

   GFP and ubiquitin

   Expression

   Yeast

   Available Since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pBigTB

   Plasmid

   #149432

   Purpose

   shuttle vector with blasticidin-S resistance into ROSA26 targetting vectors

   Depositor

   Primo Schaer

   Article

   Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

   Type

   Empty backbone

   Use

   Cre/Lox and Mouse Targeting

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMGU

   Plasmid

   #139933

   Purpose

   M. magneticum/E. coli shuttle vector (pMGT and pUC)

   Depositor

   Christopher Voigt

   Article

   Furubayashi et al Adv. Funct. Mater. 2021, 31, 2004813

   Insert

   sfGFP
   

   Expression

   Bacterial

   Promoter

   PJ23101

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBUN276

    Plasmid

    #60673

    Purpose

    overexpression of M. tuberculosis ddl gene in Mycobacterium

    Depositor

    Raul Barletta

    Article

    Feng et al Antimicrob Agents Chemother. 2003 Jan;47

    Insert

    ddl
    

    Use

    E.coli-mycobacterium shuttle vector

    Tags

    6 codons of M. bovis BCG hsp60

    Promoter

    BCG Hsp60

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. MCP-huCof K22Q + rat cof shRNA

    Plasmid

    #245959

    Purpose

    Silencing of endogenous cofilin in rat/mouse cells with replacement by human cofilin K22Q expressed with a cofilin promoter

    Depositor

    James Bamburg

    Insert

    cofilin 1 (cfl1) (CFL1 Human)
    

    Expression

    Mammalian

    Mutation

    K22Q

    Promoter

    MCP for cofilin and pol III for shRNA

    Available Since

    Oct. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJPC060

    Plasmid

    #234535

    Purpose

    TRV2 expressing FLPe

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    FLPe recombinase
    

    Use

    Recombinase

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. EV-A71 isolate 4643-TW98 2A precursor protease with C110A and four aa at N-term to create a VP1-2A junction in active site

    Plasmid

    #228633

    Purpose

    Bacterial expression of the A71 2A protease with ITTL at the N-terminal and a C110A mutation used for crystallization in P 6 1 (PDB 9FGO)

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Enterovirus Coxsackievirus A71 2A protease
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    C110A

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pJB72

    Plasmid

    #226312

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with Y to A CsoS2 MR1
    

    Expression

    Bacterial

    Mutation

    Y300A

    Promoter

    pTRC

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJB44

    Plasmid

    #226303

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with C to S MR1
    

    Expression

    Bacterial

    Mutation

    C292S, C310S

    Promoter

    pTRC

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJB43

    Plasmid

    #226302

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    WT CsoS2, frameshift site intact
    

    Expression

    Bacterial

    Promoter

    pTRC

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJBM1

    Plasmid

    #225288

    Purpose

    E. coli- C. coli shuttle vector; KanR

    Depositor

    Robert Moore

    Article

    McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

    Insert

    Replication elements from pWestmill 11
    

    Expression

    Bacterial

    Available Since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221 msNLRP3 (STOP)

    Plasmid

    #218638

    Purpose

    Gateway shuttling vector for cloning N-terminal NLRP3 tags (contains a stop codon--note: bulky N-terminal tags are likely not functional)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Gateway entry

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ301

    Plasmid

    #205415

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Hs1Cas12a without promoter

    Depositor

    Yiping Qi

    Article

    Li et al Front Genome Ed. 2023 Oct 12;5:1251903.

    Insert

    Hs1Cas12a
    

    Use

    CRISPR; Gateway compatible cas12a entry clone

    Expression

    Plant

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSM-dCas9::Ap

    Plasmid

    #154307

    Purpose

    Shuttle vector for expression dCas9, mobilization cluster, expresses the tracrRNA and a CRISPR array designed for the easy cloning of new spacers.

    Depositor

    I-Son Ng

    Article

    Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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