We narrowed to 31,889 results for: Eng

1. R26-dCas9-VPR-mNG

   Plasmid

   #172441

   Purpose

   Rosa26 targeting vector, expresses dCas9-VPR and mNeonGreen

   Depositor

   Tyler Jacks

   Article

   Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

   Inserts

   dCas9-VPR
   mNeonGreen
   

   Use

   Mouse Targeting

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. SBC009876

   Plasmid

   #226283

   Purpose

   Expresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.

   Depositor

   Nigel Scrutton

   Article

   Robinson et al Metab Eng. 2020 Jul;60:168-182. doi: 10.

   Inserts

   Coniferyl alcohol acyltransferase
   Eugenol synthase 1
   

   Expression

   Bacterial

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-ABE8e-SpCas9-HiFi-P2A-EGFP (LM446)

   Plasmid

   #197506

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HiFi(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpCas9-HiFi-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpCas9-HiFi(D10A/R69…

   Promoter

   CMV and T7

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-crRNA(TARDBP)

   Plasmid

   #109323

   Purpose

   AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TARDBP

   Depositor

   Feng Zhang

   Article

   Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

   Insert

   mCherry-KASH (TARDBP Human)
   

   Use

   AAV

   Promoter

   hSyn1

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. MKC89_CCR5(UbC-tEPOR-2A-YFP)

   Plasmid

   #232404

   Purpose

   AAV production plasmid for UbC-tEPOR-2A-YFP vector from Fig. 2 that mediates HDR at CCR5 locus using CCR5-sg3 gRNA. YFP is followed by a BGH polyA. AAV genome is flanked by AAV2 ITRs.

   Depositor

   Matthew Porteus, Kyle Cromer

   Article

   Luna et al Nat Biomed Eng. 2024 Dec;8(12):1540-1552

   Insert

   Truncated Erythropoietin Receptor (EPOR Human)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. MKC91_tEPOR(UbC-GFP-BGH)

   Plasmid

   #232406

   Purpose

   AAV production plasmid for tEPOR-UbC-GFP-BGH vector from Fig. 1 that mediates HDR at EPOR locus using EPOR-sg1 gRNA. Repair vector introduces premature stop codon into EPOR locus.

   Depositor

   Matthew Porteus, Kyle Cromer

   Article

   Luna et al Nat Biomed Eng. 2024 Dec;8(12):1540-1552

   Insert

   Truncated Erythropoietin Receptor (EPOR Human)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

   Plasmid

   #185912

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8e-nSpRY-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. LV-U6-sgRNAfiller-PGK-Cre-EFS-mScarletSIIN

   Plasmid

   #172436

   Purpose

   Lentivirus, expresses Cre recombinase and mScarlet-SIINFEKL, with sgRNA cloning site

   Depositor

   Tyler Jacks

   Article

   Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

   Inserts

   Cre
   mScarlet-SIINFEKL(OVA257-264)+Ova 323-339
   

   Use

   CRISPR and Lentiviral

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)

   Plasmid

   #197510

   Purpose

   Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
   

   Use

   AAV and CRISPR

   Tags

   BPNLS and NpuN(intein)-BPNLS

   Mutation

   ABE8e mutations in TadA and nSpRY(D10A/A61R)

   Promoter

   Cbh

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-ABE8e-SpCas9-NRTH-P2A-EGFP (RMD21)

    Plasmid

    #197503

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRTH-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRTH(D10A/I32…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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