We narrowed to 15,050 results for: pET

1. pFS_0356_pET30-RT-CRISPR_Candidatus_Accumulibacter_sp._SK-02_ARRAY_1_RC

   Plasmid

   #116968

   Purpose

   Expresses CaSK-02RT-Cas1-Cas2 under pT7lac promoter, encodes CaCRISPR array 1 RC, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Candidatus Accumulibacter sp. SK-02 RT-Cas1-Cas2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFS_0355_pET30-RT-CRISPR_Candidatus_Accumulibacter_sp._SK-02_ARRAY_1

   Plasmid

   #116967

   Purpose

   Expresses CaSK-02RT-Cas1-Cas2 under pT7lac promoter, encodes CaCRISPR array 1, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Candidatus Accumulibacter sp. SK-02 RT-Cas1-Cas2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET29b-clbH-C-A2-PCP S1524A

   Plasmid

   #114158

   Purpose

   Expresses ClbH-C-A2-PCP S1524A

   Depositor

   Emily Balskus

   Article

   Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

   Insert

   clbH
   

   Tags

   S-Tag, Thrombin site

   Expression

   Bacterial

   Mutation

   Only contains the C-A2-PCP domains of ClbH with S…

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a_6HT-TBP6.7(delta C-term)

   Plasmid

   #112731

   Purpose

   This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(delta C-term)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   delta C-term

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-30a(+)-Syk-tSH2-FX

   Plasmid

   #111272

   Purpose

   express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20-amino-acid linker (GGS)3GS(GGS)3, in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

   Insert

   murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20aa linker (Syk Mouse)
   

   Expression

   Bacterial

   Mutation

   interdomain A (Phe 119 to His 162) substituted by…

   Promoter

   T7 promoter

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET22b-ecDHFR-dCys-T46C

   Plasmid

   #110543

   Purpose

   Bacterial expression of E.coli DHFR C85A/C152S/T46C mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

   Insert

   ecDHFR
   

   Expression

   Bacterial

   Mutation

   T46 mutated to C46, C85 mutated to A85, C152 muta…

   Promoter

   T7

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET22b-ecDHFR-dCys-L54C

   Plasmid

   #110542

   Purpose

   Bacterial expression of E.coli DHFR C85A/C152S/L54C mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

   Insert

   ecDHFR
   

   Expression

   Bacterial

   Mutation

   L54 mutated to C54, C85 mutated to A85, C152 muta…

   Promoter

   T7

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET22b-ecDHFR-D27S/Y100F

   Plasmid

   #110827

   Purpose

   Bacterial expression of E.coli DHFR D27S/Y100F mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

   Insert

   ecDHFR
   

   Expression

   Bacterial

   Mutation

   D27 mutated to S27, Y100 mutated to F100

   Promoter

   T7

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28b-NmCas9-Flag D16A

   Plasmid

   #109031

   Purpose

   D16A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

   Depositor

   Yan Zhang

   Article

   Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

   Insert

   CRISPR-associated endonuclease Cas9
   

   Tags

   Flag Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETDuet RagA(R37P)-RagC

    Plasmid

    #99646

    Purpose

    Bacterial expression of RagA(R37P)-RagC

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    R37P

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETDuet RagA-RagC(K84T))

    Plasmid

    #99648

    Purpose

    Bacterial expression of RagA-RagC(K84T)

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    K84T

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pet151D-TOPO-BROX 1-401

    Plasmid

    #89866

    Purpose

    Expresses human BROX residues 1-401 in bacteria; internal ID WISP11-297

    Depositor

    Wesley Sundquist

    Article

    Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

    Insert

    BROX (BROX Human)
    

    Tags

    His Tag, V5 tag

    Expression

    Bacterial

    Mutation

    expresses only residues 1-401

    Promoter

    T7

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. csd2_121-308,S166A/pET28b(+)

    Plasmid

    #83301

    Purpose

    csd2, construct: 121-308, pET28b(+) NC-tag, mutation: S166A

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-120, and changed Serine 166 …

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. csd2_121-308,T151A/pET28b(+)

    Plasmid

    #83300

    Purpose

    csd2, construct: 121-308, pET28b(+) NC-tag, mutation: T151A

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-120, and changed Threonine 1…

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. csd2_121-308,Y197F/pET28b(+)

    Plasmid

    #83299

    Purpose

    csd2, construct: 121-308, pET28b(+) NC-tag, mutation: Y197F

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-120, and changed Tyrosine 19…

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. csd2_121-308,E165A/pET28b(+)

    Plasmid

    #83298

    Purpose

    csd2, construct: 121-308, pET28b(+) NC-tag, mutation: E165A

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-120, and changed Glutamic ac…

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-Hook3 D102A, E108A

    Plasmid

    #74618

    Purpose

    Expresses a double mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)
    

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    D102A, E108A

    Promoter

    T7

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21a-Chaetomium thermophilum LIC

    Plasmid

    #74782

    Purpose

    encodes dynein light intermediate chain (LIC) from Chaetomium thermophilum; no tag

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    Chaetomium thermophilum dynein light intermediate chain
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJK040 (pET-PurQ-A128T)

    Plasmid

    #73840

    Purpose

    Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)

    Depositor

    T. J. Kappock, JoAnne Stubbe

    Article

    Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

    Insert

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    

    Expression

    Bacterial

    Mutation

    A128T mutant

    Promoter

    T7

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. csd6_17-330/pET28b(+) N-tag

    Plasmid

    #71353

    Purpose

    csd6, construct:17-330, pET28b(+) N-tag (Please note that this plasmid has a C-terminal His tag)

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 )
    

    Tags

    Histidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

21. csd6_4-330/pET28b(+) N-tag

    Plasmid

    #71351

    Purpose

    csd6, construct:4-330, pET28b(+) N-tag

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 )
    

    Tags

    Histidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

22. csd6_13-330/pET28b(+) N-tag

    Plasmid

    #71352

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 )
    

    Tags

    Histidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

23. csd6_13-330_E110A/pET28b(+) N-tag

    Plasmid

    #71354

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:E110A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Glutamic acid 110 to Alanine)
    

    Tags

    Histidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

24. csd6_13-330_Y133A/pET28b(+) N-tag

    Plasmid

    #71356

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:Y133A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Tyrosine 133 to Alanine)
    

    Tags

    Histidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

25. pETM6-Vv4CL-PhCHS-MsCHI

    Plasmid

    #73400

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    Vv4CL
    PhCHS
    MsCHI
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pETM6-Vv4CL-CmCHS-CmCHI

    Plasmid

    #73403

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    Vv4CL
    CmCHS
    CmCHI
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pETM6-Pc4CL-CmCHS-CmCHI

    Plasmid

    #73399

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    Pc4CL
    CmCHS
    CmCHI
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. csd6_13-330_C176A/pET28b(+) N-tag

    Plasmid

    #71360

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:C176A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Cysteine 176 to Alanine)
    

    Tags

    Histidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

29. csd6_13-330_H160A/pET28b(+) N-tag

    Plasmid

    #71359

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:H160A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Histidine 160 to Alanine)
    

    Tags

    Histidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

30. csd6_13-330_W158A/pET28b(+) N-tag

    Plasmid

    #71358

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:W158A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Tryptophan 158 to Alanine)
    

    Tags

    Histidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only