178,119 results
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Plasmid#82612PurposeExpress PAsfCherry2(1-10) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPAsfCherry2(1-10)
ExpressionMammalianMutationchanged Glu 118 to Gln, changed Thr 128 to IlePromoterCMVAvailable sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP
Plasmid#85165PurposeProvides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.DepositorInsertEcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
TagsAmilCP and RTXExpressionBacterialPromoterJ23100 promoterAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Dazl-CPG-TV
Plasmid#70144Purposea targeting vector, to target the endogenous DAZL CPG island with Snrp-GFP-polyADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert5'hDAZL-snrp-GFP-polyA-pgK-PURO-3'hDAZL
UseMouse Targeting and Synthetic BiologyAvailable sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)
Plasmid#85041PurposeThis plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertloxP-stop-loxP
UseCRISPRExpressionMammalianAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5E-2.3k-fabp2
Plasmid#84927Purpose–2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5’ Entry vectorDepositorAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSEVA331Bb
Plasmid#78269PurposepSEVA331 with Biobrick multiple cloning siteDepositorTypeEmpty backboneAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAS9-mCherry empty
Plasmid#80975PurposeCas9, mCherry, and sgRNA expressionDepositorTypeEmpty backboneExpressionMammalianAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDF-His-mTET1CD
Plasmid#81053Purposebacterial expression of the catalytic domain of murine TET1DepositorInsertTET1 (Tet1 Mouse)
Tags6HISExpressionBacterialMutationcatalytic domain amino acid 1367-2057PromoterT7Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
WN10151
Plasmid#80441PurposeExpresses human codon-optimized dead (832A) LbCpf1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdead (D832A) LbCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationD832AAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.cytGAP
Plasmid#78734PurposeExpression of Ca2+ sensor GAP in the cytosolDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcytGAP
TagsluciferaseExpressionMammalianPromoterCMVAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by