We narrowed to 128 results for: spycatcher

1. pIBA2-SpyTag-MBP

   Plasmid

   #107422

   Purpose

   Control construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.

   Depositor

   Jack Leo

   Article

   Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

   Insert

   Maltose-binding protein (MalE)
   

   Use

   Tags

   OmpA signal peptide, SpyTag, and StrepII

   Expression

   Bacterial

   Mutation

   Promoter

   Tet

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. LLP782_Traptavidin-KRAB-CO

   Plasmid

   #211775

   Purpose

   AviTag counterpart binding domain, Traptavidin, fused to transcriptional repressor KRAB, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   SpyCatcher-DNMT3A
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect DNMT3A…

   Promoter

   pEF1a and pSV40

   Available since

   Feb. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST14-DoubleCatcher

   Plasmid

   #216284

   Purpose

   Expresses SpyCatcher003-SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow stepwise coupling of two unique SpyTag-linked binders

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. InterCatch-3xL

   Plasmid

   #186909

   Purpose

   Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through (GGGGS)3 linker and the other side expressing EGFP and puromycin resistance gene

   Depositor

   Allen Liu, Taekjip Ha

   Article

   Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

   Insert

   TfR-(GGGGS)3-SpyCatcher003-sfCherry1-10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. InterCatch-2xL

   Plasmid

   #186908

   Purpose

   Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through (GGGGS)2 linker and the other side expressing EGFP and puromycin resistance gene

   Depositor

   Allen Liu, Taekjip Ha

   Article

   Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

   Insert

   TfR-(GGGGS)2-SpyCatcher003-sfCherry1-10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. InterCatch

   Plasmid

   #186906

   Purpose

   Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR and the other side expressing EGFP and puromycin resistance gene

   Depositor

   Allen Liu, Taekjip Ha

   Article

   Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

   Insert

   TfR-SpyCatcher003-sfCherry1-10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. InterCatch-1xL

   Plasmid

   #186907

   Purpose

   Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through a single GGGGS linker and the other side expressing EGFP and puromycin resistance gene

   Depositor

   Allen Liu, Taekjip Ha

   Article

   Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

   Insert

   TfR-(GGGGS)1-SpyCatcher003-sfCherry1-10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Halo-TMD

   Plasmid

   #195347

   Purpose

   Fluorescent labeling for cell membranes

   Depositor

   Allen Liu

   Article

   Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

   Insert

   HaloTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST14-DoubleCatcher-H-Lock

   Plasmid

   #216285

   Purpose

   Expresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher H-Lock
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST14-DoubleCatcher-⍺-Lock

    Plasmid

    #216286

    Purpose

    Expresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher ⍺-Lock
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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