We narrowed to 134 results for: spycatcher
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Plasmid#186902PurposeCell-free expression of soluble protein sfGFP-SpyCatcher-sfCherry1-10DepositorInsertsfGFP-SpyCatcher003-sfCherry1-10
UseSynthetic BiologyTagssfGFPAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
sCatch-GFP-6xHIS
Plasmid#186904PurposeBacterial expression of soluble protein sfGFP-SpyCatcher-sfCherry1-10 with a 6xHIS tagDepositorInsertsfGFP-SpyCatcher003-sfCherry1-10-6xHIS
Tags6xHis and sfGFPExpressionBacterialAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-MMP
Plasmid#112632PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-MMP
TagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-sfGFP
Plasmid#184226PurposeExpresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.DepositorInsertSpyTag-sfGFP
TagsHis6, SpyTag, and TEV protease cleavage siteExpressionBacterialPromoterT5Available SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP-MMP
Plasmid#112633PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP-MMP
TagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFBD-SpyTag-noro-VLP
Plasmid#165989PurposeExpresses norovirus-like particles with SpyTags presented on their surface for convenient decoration of SpyCatcher fusion proteinsDepositorInsertSpyTag-noro-VLP (ORF2 Synthetic, norovirus)
TagsSpyTagExpressionInsectPromoterPolyhedrinAvailable SinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIBA2-SpyTag-MBP
Plasmid#107422PurposeControl construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.DepositorInsertMaltose-binding protein (MalE)
TagsOmpA signal peptide, SpyTag, and StrepIIExpressionBacterialPromoterTetAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
LLP782_Traptavidin-KRAB-CO
Plasmid#211775PurposeAviTag counterpart binding domain, Traptavidin, fused to transcriptional repressor KRAB, with GFP selectionDepositorInsertSpyCatcher-DNMT3A
Tags3xFLAGExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available SinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher
Plasmid#216284PurposeExpresses SpyCatcher003-SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow stepwise coupling of two unique SpyTag-linked bindersDepositorInsertDoubleCatcher
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
InterCatch-3xL
Plasmid#186909PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through (GGGGS)3 linker and the other side expressing EGFP and puromycin resistance geneDepositorInsertTfR-(GGGGS)3-SpyCatcher003-sfCherry1-10
ExpressionMammalianAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only