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AAVS1-Pur-CAG-EGFP

Plasmid

#80945

Purpose

human AAVS1 site targeting donor plasmid for knocking-in EGFP expression cassette driven by CAG promoter

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-GFP(S65T)-kanMX6

Plasmid

#39292

Depositor

John Pringle, Jurg Bahler

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

KanR

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Mutation

Promoter

Available since

Aug. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pAS_4xG1 PylT sfGFP 150TAG

Plasmid

#154767

Purpose

plasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pR26 GFP Dest

Plasmid

#74283

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Tags

Expression

Mutation

Promoter

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pNOC-CRISPR-GFP

Plasmid

#100009

Purpose

Expresses Cas9-GFP and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into geneome

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis expression

Tags

Cas9-GFP

Expression

Mutation

Promoter

Ribi promoter

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLV GG hUbV-EGFP

Plasmid

#216161

Purpose

Contains a Golden-Gate cloning cassette to express up to four gRNA and EGFP

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-EGFP-APOBEC1

Plasmid

#209321

Purpose

AAV packaging vector containing a EGFP-P2A expression cassette, APOBEC1 expression.

Depositor

Magdalena Koziol

Article

Feng et al Genome Res. 2024 Jul 23;34(6):822-836. d

Insert

APOBEC1-HA

Use

AAV

Tags

HA

Expression

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLV.5’eGFP/sgLMNA

Plasmid

#170546

Purpose

Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP without homology arms

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Insert

sgRNA targeting 5'- end of LMNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-GFP-HS

Plasmid

#133305

Purpose

"Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisation

Depositor

Chris Barnes

Article

Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

Insert

hok/sok toxin-antitoxin cassette

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBBK96 pHomL-ConLS-GFPdropout-ConR1-HomR (AmpR)

Plasmid

#179080

Purpose

Parent vector for for creating new markerless yeast integration cassettes with different promoters/genes/tags/terminators

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_EGFP

Plasmid

#179392

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of EGFP

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

EGFP

Use

Tags

AviTag

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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Ai161 (TIT2L-GFP-ICR-tTA2) targeting vector

Plasmid

#114429

Purpose

Target a Cre-dependent GFP cassette and Dre-dependent tTA2 cassette to the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GFP

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mutation

Promoter

TRE2, CAG

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pR26 CAG/GFP Dest

Plasmid

#74281

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pR26 CAG/GFP Asc

Plasmid

#74285

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscI

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLV U6-sgRNA UbC-GFP

Plasmid

#106948

Purpose

sgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP marker

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

sgRNA BsmBI/Esp3I cloning site

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAS_4xhyb PylT sfGFP 150TAG

Plasmid

#154771

Purpose

plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG

Plasmid

#174892

Purpose

amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG in sfGFP

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLV.5’eGFP/sgVIM

Plasmid

#170548

Purpose

Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP without homology arms

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Insert

sgRNA targeting 5'- end of VIM

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UUA)_EF1_sfGFP150TAA

Plasmid

#174897

Purpose

ochre suppression reporter sfGFP150TAA expression, with MmaPylT(UUA) ochre suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sf GFP

Use

Tags

Expression

Mammalian

Mutation

150TAA in sfGFP

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UCA)_EF1_sfGFP150TGA

Plasmid

#174898

Purpose

opal suppression reporter sfGFP150TGA expression, with MmaPylT(UCA) opal suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TGA in sfGFP

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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