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Plasmid#29723DepositorTypeEmpty backboneUseTags6xHis, AviTag (biotin), TEV cleavage site, and mO…ExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only
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pET Biotin His6 mCitrine LIC cloning vector (H6-mCitrine)
Plasmid#29724DepositorTypeEmpty backboneUseTags6xHis, AviTag (biotin), TEV cleavage site, and mC…ExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 FlAsH LIC cloning vector (H6-FlAsH)
Plasmid#30184DepositorTypeEmpty backboneUseTags6xHis, AviTag (biotin), FlAsH, and TEV cleavage s…ExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mOrange LIC cloning vector (MBP-mOrange)
Plasmid#29748DepositorTypeEmpty backboneUseTags6xHis, AviTag (biotin), MBP, TEV cleavage site, a…ExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mCerulean LIC cloning vector (MBP-mCerulean)
Plasmid#29751DepositorTypeEmpty backboneUseTags6xHis, AviTag (biotin), MBP, TEV cleavage site, a…ExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
PP-CDPK1 (3Q5I)
Plasmid#28280DepositorInsertPP-CDPK1 (PB000158.03.0 Plasmodium berghei)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET MBP mCitrine LIC cloning vector (MBP-mCitrine)
Plasmid#29749DepositorTypeEmpty backboneUseTags6xHis, AviTag (biotin), MBP, TEV cleavage site, a…ExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pJK681
Plasmid#72271PurposeProduces Synechocystis PCC6803 orf sll1489 (synthetic gene)DepositorInserthypothetical protein SYNGTS_3005
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK588
Plasmid#72270PurposeSynthetic gene corresponding to Synechocystis PCC6803 orf sll1489DepositorInserthypothetical protein SYNGTS_3005
UseGene cloningTagsHis6 and TEV cleavage siteExpressionMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-10
Plasmid#72348PurposeMammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…ExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-BB0238-ss
Plasmid#137046PurposeE. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66635.2 (BB_0238 Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature BB0238: lacks the BB0238 signal peptidePromoterT7lacAvailable sinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-P66-ss
Plasmid#137045PurposeE. coli expression clone (T7lac promoter) for mature P66 (aa 22-618) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66949.1 (p66 Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature P66: lacks the P66 signal peptidePromoterT7lacAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-P13-ss
Plasmid#137065PurposeE. coli expression clone (T7lac promoter) for mature P13 (aa 22-179) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66426.1 (BB_0034 Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature P13: lacks the P13 signal peptidePromoterT7lacAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
ATX3-13Q
Plasmid#184248PurposeRecombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)DepositorInsertataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Human, Synthetic)
UseTags6x His-Tag and TEV cleavage siteExpressionBacterialMutationC-terminal codon optimization for protein expres…PromoterT7 promoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-RevA-ss
Plasmid#137064PurposeE. coli expression clone (T7lac promoter) for mature RevA (aa 25-160) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAF07416.1 (revA Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationlacks the RevA signal peptide and 5 residues (CKA…PromoterT7lacAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-HtrA-ss
Plasmid#137066PurposeE. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66500.2 (BB_0104 Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature HtrA: lacks the HtrA signal peptidePromoterT7lacAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-DipA-ss
Plasmid#137068PurposeE. coli expression clone (T7lac promoter) for mature DipA (aa 21-342) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66790.1 (BB_0418 Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature DipA: lacks the DipA signal peptidePromoterT7lacAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
KRAS-V14I
Plasmid#154427PurposeV14I Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.DepositorInsertKRAS (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGFP-HsSyx3
Plasmid#179289PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + GFP + TEV protease cleavage siteDepositorInsertSyx
UseTagsHis6 + GFP + TEV protease cleavage siteExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7Available sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only