We narrowed to 31,528 results for: Eng;

1. pBbA4k-RFP

   Plasmid

   #35300

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ProE

   Available since

   Feb. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBbE4k-RFP

   Plasmid

   #35297

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ProE

   Available since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p44PPV-eGFP

   Plasmid

   #241907

   Purpose

   Part 2 of a two-plasmid PCA biosensor composed of the PcaV repressible PPV promoter upstream from a reporter gene (eGFP) on a second plasmid

   Depositor

   Neil Dixon

   Article

   F M Machado et al J Biol Eng. 2019 Nov 27;13:91. doi: 10.1

   Insert

   His6-eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   none

   Promoter

   Available since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCfB11785

   Plasmid

   #212699

   Purpose

   gRNA targeting to the intergradtion site E4

   Depositor

   Irina Borodina

   Article

   Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

   Insert

   gRNA targeting to the intergradtion site E4
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCfB10769

   Plasmid

   #212694

   Purpose

   gRNA targeting to the intergradtion site E2

   Depositor

   Irina Borodina

   Article

   Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

   Insert

   gRNA targeting to the intergradtion site E2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCfB12719

   Plasmid

   #212702

   Purpose

   gRNA targeting to the intergradtion site F3

   Depositor

   Irina Borodina

   Article

   Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

   Insert

   gRNA targeting to the intergradtion site F3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCfB12720

   Plasmid

   #212704

   Purpose

   gRNA targeting to the gene 4HPPD locus

   Depositor

   Irina Borodina

   Article

   Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

   Insert

   gRNA targeting to the gene 4HPPD locus
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCfB11787

   Plasmid

   #212695

   Purpose

   gRNA targeting to the intergradtion site E2

   Depositor

   Irina Borodina

   Article

   Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

   Insert

   gRNA targeting to the intergradtion site E2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCfB6637

   Plasmid

   #212696

   Purpose

   gRNA targeting to the intergradtion site E3

   Depositor

   Irina Borodina

   Article

   Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

   Insert

   gRNA targeting to the intergradtion site E3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCfB8860

    Plasmid

    #212697

    Purpose

    gRNA targeting to the intergradtion site E3

    Depositor

    Irina Borodina

    Article

    Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

    Insert

    gRNA targeting to the intergradtion site E3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCfB8856

    Plasmid

    #212701

    Purpose

    gRNA targeting to the intergradtion site C3

    Depositor

    Irina Borodina

    Article

    Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

    Insert

    gRNA targeting to the intergradtion site C3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCfB8866

    Plasmid

    #212703

    Purpose

    gRNA targeting to the intergradtion site F3

    Depositor

    Irina Borodina

    Article

    Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

    Insert

    gRNA targeting to the intergradtion site F3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCfB6630

    Plasmid

    #212700

    Purpose

    gRNA targeting to the intergradtion site C3

    Depositor

    Irina Borodina

    Article

    Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

    Insert

    gRNA targeting to the intergradtion site C3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCfB6638

    Plasmid

    #212698

    Purpose

    gRNA targeting to the intergradtion site E4

    Depositor

    Irina Borodina

    Article

    Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

    Insert

    gRNA targeting to the intergradtion site E4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTS1037

    Plasmid

    #169616

    Purpose

    Tier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven expression of secreted Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTS1035

    Plasmid

    #169614

    Purpose

    Tier-2 vector encoding PmPGK1-driven secreted Nluc (PmPGK1-IgkSS-Nluc-pA::A2-pA::A3-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven expression of secreted Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTS1032

    Plasmid

    #169612

    Purpose

    Tier-2 vector encoding PhCMV-driven secreted Nluc (A1-pA::PhCMV-IgkSS-Nluc-pA::A3-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of secreted Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTS1031

    Plasmid

    #169611

    Purpose

    Tier-2 vector encoding PhCMV-driven secreted Nluc (PhCMV-IgkSS-Nluc-pA::A2-pA::A3-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of secreted Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTS1033

    Plasmid

    #169613

    Purpose

    Tier-2 vector encoding PhCMV-driven secreted Nluc (A1-pA::A2-pA::PhCMV-IgkSS-Nluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of secreted Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTS1036

    Plasmid

    #169615

    Purpose

    Tier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::PmPGK1-IgkSS-Nluc-pA::A3-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven expression of secreted Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCI-T7Max-UTR1-T500

    Plasmid

    #178418

    Purpose

    Empty backbone. T7Max promoter

    Depositor

    Kate Adamala

    Article

    Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7Max

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCI-T7Max-UTR1-NTerminus8xHis-T500

    Plasmid

    #178421

    Purpose

    Empty backbone. T7Max promoter with N-His Tag

    Depositor

    Kate Adamala

    Article

    Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7Max

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCTCON2-GXB

    Plasmid

    #160926

    Purpose

    Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Inserts

    BFP
    sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCTCON2-Aga1p-FAPB2.3.6L1TAG

    Plasmid

    #158143

    Purpose

    Yeast display reporter for non-display yeast strains (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    FAPB2.3.6L1TAG
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PHBA03

    Plasmid

    #83537

    Purpose

    Constitutive TEF1 promoter mediated expression of the UBiC, ARO4*, and TKL1 genes

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

    Insert

    pTEF1-UBiC-CYC1t-pTEF1-ARO4*-CYC1t-pTEF1-TKL1-CYC1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCas73

    Plasmid

    #82398

    Purpose

    Δccm:Km, used to delete ccm operon

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    ccm deletion
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDS3-aurJ

    Plasmid

    #70152

    Purpose

    Fusarium graminearum AurJ encoded on CEN/ARS yeast shuttle vector with DS3 selection marker

    Depositor

    Morten Sommer

    Article

    Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

    Insert

    aurJ
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD1

    Available since

    Dec. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pDS2

    Plasmid

    #70135

    Purpose

    CEN/ARS yeast shuttle vector with DS2 selection marker

    Depositor

    Morten Sommer

    Article

    Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

    Insert

    DS2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    AgTEF1

    Available since

    Dec. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBbB0a-RFP

    Plasmid

    #35371

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBbB0k-RFP

    Plasmid

    #35370

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBbS3c-RFP

    Plasmid

    #35311

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ProS

    Available since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBbE3c-RFP

    Plasmid

    #35305

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ProS

    Available since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHB-4

    Plasmid

    #140957

    Purpose

    For PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.

    Depositor

    Kang Zhou

    Article

    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

    Insert

    phaCAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMP11

    Plasmid

    #215559

    Purpose

    pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Inserts

    Cas9
    gRNA targeting pBR322 origin of replication
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23105 and tet

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRK793

    Plasmid

    #8827

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    TEV protease, S219V mutant
    

    Use

    Tags

    His, MBP (with TEV), and polyarginine

    Expression

    Bacterial

    Mutation

    S219V mutation (improves stability)

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pETM6-T7-VHH-GFPminimizer

    Plasmid

    #202488

    Purpose

    Expresses the nanobody GFP minimizer with T7 induction

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody GFP minimizer
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRK792

    Plasmid

    #8830

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    TEV protease, S219P mutant
    

    Use

    Tags

    His, MBP (cleaved), and polyarginine

    Expression

    Bacterial

    Mutation

    S219P mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHB-3

    Plasmid

    #140956

    Purpose

    For PHB production in E. coli (T7-pAC). Please note that this is listed as #140955 in the publication.

    Depositor

    Kang Zhou

    Article

    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

    Insert

    phaCAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. LYS-Leu2

    Plasmid

    #218114

    Purpose

    Integration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pVH41-Tier1-PhCMV-GLuc

    Plasmid

    #169544

    Purpose

    Tier-1 vector encoding PhCMV-driven gaussia luciferase (PhCMV-GLuc-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven Gaussia Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge