Addgene

We narrowed to 1,214 results for: ADAP

Showing: 221 - 240 of 1214 results

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USP19 sgRNA1

Plasmid

#78585

Purpose

delete USP19 gene in human cell

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

USP19 sgRNA1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CBh

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
YIplac211-MNN9-msGFP2

Plasmid

#177105

Purpose

Expresses Mnn9-msGFP2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

MNN9 (MNN9 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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qTAG-C-miniTurbo-Zeo

Plasmid

#207736

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Zeo-miniTurbo

Plasmid

#207703

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-miniTurbo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Zeo-3xFLAG

Plasmid

#207706

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xFLAG cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Blast-3xHA

Plasmid

#207685

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xHA cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
YIplac211-HaloTag-DRS2

Plasmid

#177103

Purpose

Expresses HaloTag-Drs2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

DRS2 (DRS2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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YIplac211-ANP1-msGFP2

Plasmid

#177100

Purpose

Expresses Anp1-msGFP2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

ANP1 (ANP1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-rasmKate

Plasmid

#105938

Purpose

in vitro transcription

Depositor

Caren Norden

Article

Weber et al Cell Rep. 2014 Apr 24;7(2):386-97. doi:

Insert

Ras

Use

Tags

mKate

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-Cyc1t (17-21)

Plasmid

#101700

Purpose

Cyc1t in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GCTT-[terminator of S. cerevisiae YJR048W (190 bp)]-CGCT

Use

Yeast expression

Tags

Expression

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-pTEF2 (01-11)

Plasmid

#101685

Purpose

pTEF2 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YBR118W (520bp)]-CCAT

Use

Yeast expression

Tags

Expression

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-SYS1-msGFPx3

Plasmid

#177112

Purpose

Expresses Sys1-msGFP3 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

SYS1 (SYS1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-ENT5-HaloTag

Plasmid

#177102

Purpose

Expresses Ent5-HaloTag in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

ENT5 (ENT5 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-calx1

Plasmid

#58697

Purpose

Expression of calx1

Depositor

Craig Montell

Article

Wang et al Neuron 2005 Feb 3;45(3):367-78.

Insert

calx1 (Calx Fly)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-PRLSS-SH2-KDEL

Plasmid

#209716

Purpose

Mammalian expression of SH2 localized to endoplasmic reticulum

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

PRLSS-SH2-KDEL

Use

Tags

PRLSS

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huLb5Cas12a

Plasmid

#174684

Purpose

Expresses 6xHis-MBP tagged Lb5Cas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huLb5Cas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-MyD88-FW/AA

Plasmid

#12289

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

MyD88 (MYD88 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

F285A/W286A

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
6xHis-MBP-huHkCas12a

Plasmid

#174683

Purpose

Expresses 6xHis-MBP tagged HkCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huHkCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-neo-AP-4 (Myc)3-σ4

Plasmid

#198331

Purpose

Expresses the triple Myc-tagged mouse AP-4 σ4 subunit in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-4 σ4

Use

Tags

triple Myc tag

Expression

Mammalian

Mutation

silent substitution in codon 131

Promoter

CMV

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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Cx55.5:rasGFP

Plasmid

#105939

Purpose

in vitro transcription

Depositor

Caren Norden

Article

Weber et al Cell Rep. 2014 Apr 24;7(2):386-97. doi:

Insert

Ras

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge