We narrowed to 7,568 results for: Lif

1. pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

   Plasmid

   #175526

   Purpose

   Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Lifeact-HaloTag11 and Tomm20-HaloTag7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag11 = HaloTag7-M175W

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTub-alpha1-EB3-NeonGreen-T2A-iLifeact-mCherry

   Plasmid

   #175261

   Purpose

   labelling of microtubule plus end and F-actin structures

   Depositor

   Sebastián Dupraz, Frank Bradke

   Article

   Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

   Insert

   EB3-NeonGreen-T2A-iLifeact-mCherry (MAPRE3 Human)
   

   Use

   Tags

   mCherry (on iLifeact) and mNeonGreen (on EB3)

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   pTub-alpha1

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721)

   Plasmid

   #229826

   Purpose

   Tet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mScarlet-I
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GIL-6

   Plasmid

   #213389

   Purpose

   Expresses a chimeric cytokine between IL-6 and LIF

   Depositor

   Jurgen Scheller

   Article

   Rafii et al J Biol Chem. 2024 May;300(5):107251. doi

   Insert

   GIL-6
   

   Use

   Tags

   IgG1-Fc-tag and Myc-tg

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1 GIL-11 myc TEV TwinStrep

   Plasmid

   #199627

   Purpose

   Eucaryotic expression plasmid for chimeric cytokine of IL-11 and LIF (GIL-11) with a TwinStrep-tag

   Depositor

   Jurgen Scheller

   Article

   Rafii et al Commun Biol. 2023 Apr 15;6(1):418. doi:

   Insert

   GIL-11
   

   Use

   Tags

   TwinStrep-tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. scAAV-TTR-mFgf15

   Plasmid

   #190594

   Purpose

   AAV construct containing Fgf15 cds under control of TTR promoter

   Depositor

   Roel Nusse

   Article

   Sarkar et al Elife. 2023 Jan 31;12:e82311. doi: 10.75

   Insert

   Fgf15 (Fgf15 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ttr

   Available since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDYH5

   Plasmid

   #52032

   Purpose

   Expression of GFP-tagged DAF-16 in C. elegans

   Depositor

   Meng-Qiu Dong

   Article

   Tao et al Elife. 2013;2:e00518. doi: 10.7554/eLife

   Insert

   DAF-16 (daf-16 Nematode)
   

   Use

   Tags

   GFP and HIS

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 6, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pTL-4

   Plasmid

   #52033

   Purpose

   Inducible expression of GST-unc43 in E. coli

   Depositor

   Meng-Qiu Dong

   Article

   Tao et al Elife. 2013;2:e00518. doi: 10.7554/eLife

   Insert

   Unc-43 (unc-43 Nematode)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pGI3EM22C

   Plasmid

   #135488

   Purpose

   Agrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hph

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   hph SpunH2A/Bpr LifeAct-tdTomato
   

   Use

   Tags

   LifeAct

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpunH2A/Bpr (divergent)

   Available since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAP28

    Plasmid

    #194707

    Purpose

    Linker::3xFlag::mScarlet::HAtag::Linker

    Depositor

    Alexandre Paix

    Article

    Seleit et al Elife. 2021 Dec 6;10. pii: 75050. doi: 1

    Insert

    Linker::3xFlag::mScarlet::HAtag::Linker
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAP27

    Plasmid

    #194706

    Purpose

    Linker::3xFlag::mNeonGreen::HAtag::Linker

    Depositor

    Alexandre Paix

    Article

    Seleit et al Elife. 2021 Dec 6;10. pii: 75050. doi: 1

    Insert

    Linker::3xFlag::mNeonGreen::HAtag::Linker
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. NK803

    Plasmid

    #166057

    Purpose

    Puromycin resistance and LifeAct-mCherry expression for Salpingoeca rosetta

    Depositor

    Nicole King

    Article

    Brunet et al Elife. 2021 Jan 15;10. pii: 61037. doi:

    Inserts

    Pac puromycin resistance gene
    LifeAct-mCherry
    

    Use

    S. rosetta expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNP151

    Plasmid

    #106359

    Purpose

    To insert a single copy of a construct at a site on Chr II, corresponding to a mKate-labelled LifeAct expressed specifically in the adult epidermis.

    Depositor

    Jonathan Ewbank

    Article

    Taffoni et al Elife. 2020 Jan 29;9. pii: 45047. doi: 1

    Insert

    col-62p_lifeact_mkate2_3'nmy-2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO.1 3xHA-KillerRed-OMP25

    Plasmid

    #174544

    Purpose

    localizes KillerRed to mitochondria

    Depositor

    Minna Roh-Johnson

    Article

    Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

    Insert

    3xHA-KillerRed-OMP25
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO.1 mito-mEmerald

    Plasmid

    #174542

    Purpose

    labels mitochondria with mEmerald

    Depositor

    Minna Roh-Johnson

    Article

    Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

    Insert

    MTS-mEmerald
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO.1 mito-TagRFP-T

    Plasmid

    #174543

    Purpose

    labels mitochondria with TagRFP-T

    Depositor

    Minna Roh-Johnson

    Article

    Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

    Insert

    MTS-tagRFP-T
    

    Use

    Lentiviral

    Tags

    tagRFP-T

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJP118

    Plasmid

    #176494

    Purpose

    Expresses Lifeact-mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter

    Depositor

    Duojia Pan

    Article

    Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

    Insert

    Lifeact peptide
    

    Use

    Capsaspora owczarzaki expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pmCitrine_N1

    Plasmid

    #92420

    Purpose

    Empty backbone for C-terminal fusion of mCitrine

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    mCitrine
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Stx4-pmCitrine

    Plasmid

    #92422

    Purpose

    Expression of syntaxin-4 C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    Stx4 (Stx4 Rat)
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Stx3-pmCitrine

    Plasmid

    #92421

    Purpose

    Expression of syntaxin-3 C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    Stx3 (Stx3 Rat)
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge