We narrowed to 2,329 results for: PUC19

1. pUC19 gene 60 to gap + Cys

   Plasmid

   #49159

   Purpose

   Template for generating gene 60 mRNA, sequence 5' of coding gap + Cys

   Depositor

   Nils Walter

   Article

   Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

   Insert

   Bacteriophage T4 gene 600
   

   Mutation

   Coding gap and downstream sequence have been dele…

   Promoter

   T7

   Available Since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. puC19 preACT1(del6) A259C

   Plasmid

   #111329

   Purpose

   T7 transcription

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Expression

   Bacterial

   Mutation

   branch site A259C

   Availability

   Academic Institutions and Nonprofits only

   

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3. puC19 preACT1(del6) C256A

   Plasmid

   #111331

   Purpose

   T7 transcription

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Expression

   Bacterial

   Mutation

   branch site C256A

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-P70a-Top-T~P70a-Bottom-T

   Plasmid

   #87314

   Purpose

   Expresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Split-Broccoli system
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   P70a

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19/PLIN2 GFP-P2A-BFP

   Plasmid

   #202195

   Purpose

   knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2

   Depositor

   James Olzmann

   Article

   Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

   Insert

   PLIN2 (PLIN2 Human)
   

   Use

   Cloning vector

   Tags

   GFP-P2A-BFP knock-in

   Promoter

   lac

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-TgKI-MCS-tdTomato-polyA

   Plasmid

   #174022

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   tdTomato
   

   Use

   Zebrafish knock-in tagging

   Available Since

   Nov. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19-dual-scafffold-2.0 (pBP49)

   Plasmid

   #218931

   Purpose

   The dual-scaffold for making dual-CRISPR screening libraries or dual-CRISPR plasmids.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Insert

   dual scaffolds for CRISPR
   

   Use

   CRISPR

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-eGFP-P2A-dTAG-Ing3

   Plasmid

   #170754

   Purpose

   Donor plasmid for eGFP-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Ing3 locus

   Depositor

   Kristian Helin

   Article

   Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

   Insert

   eGFP-P2A-2xHA-dTAG
   

   Use

   Mouse Targeting

   Tags

   eGFP-P2A-2xHA-dTAG

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   none

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

   Plasmid

   #223137

   Purpose

   pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIG-747pUC19-tNGFR-P2A-CD7

    Plasmid

    #186086

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD7 (CD7 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdown

    Plasmid

    #188634

    Purpose

    HDR template for CD3ε-mNeonGreen knock-in at C-terminus of human EGFR

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    EGFR (EGFR Human, Synthetic)
    

    Use

    CRISPR

    Tags

    CD3ε-mNeonGreen

    Expression

    Mammalian

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKB33 (pUC19-KANMX-tetO7-CYCpr)

    Plasmid

    #210472

    Purpose

    Plasmid with KanMX-tetO7-CYCpr cassette from the yTHC collection. Used for amplifying cassette with homology to regions of interest for genomic integration.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Insert

    KanMX-TetO7-CYCpr cassette
    

    Use

    Synthetic Biology

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIG-730pUC19-tNGFR-P2A-Coronin1A

    Plasmid

    #186110

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CORONIN1A
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIG-703pUC19-tNGFR-P2A-BACH2

    Plasmid

    #186112

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    BACH2 (BACH2 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIG-712pUC19-tNGFR-P2A-ITCH

    Plasmid

    #186085

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    ITCH (ITCH Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTR-177 pUC19-CLTA-N-mCherry

    Plasmid

    #186128

    Purpose

    knockin of mCherry to CLTA target

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CLTA (CLTA Human)
    

    Use

    CRISPR

    Mutation

    WT with mCherry sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-TgKI-MCS-mLanYFP-polyA

    Plasmid

    #174021

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    mLanYFP
    

    Use

    Zebrafish knock-in tagging

    Available Since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC19, containing H2-Kb c57/BL6 WK9

    Plasmid

    #102641

    Purpose

    Encodes sequence of the murine H2-Kb allele

    Depositor

    Sai Reddy

    Article

    Kelton et al Sci Rep. 2017 Apr 4;7:45775. doi: 10.103

    Insert

    H2-Kd Balb/c
    

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19-Split-Broccoli-Toehold-Switch

    Plasmid

    #87316

    Purpose

    Expresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system and RNA Trigger (TS2_AT01) Toehold (TS2_KS01) switch for mCherry
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    P70a

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIG-709pUC19-tNGFR-P2A-IL10

    Plasmid

    #186117

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    IL10 (IL10 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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