We narrowed to 856 results for: V2

1. pPD152 Reporter Template v2

   Plasmid

   #138752

   Purpose

   Template for COMET promoters, expresses EYFP reporter upon promoter activation. BsaI sites produce palindromic sticky ends.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti CRISPR V2 sgMTHFD1L_2

   Plasmid

   #106317

   Purpose

   Express Cas9 and sgRNA targeting MTHFD1L

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   sgRNA targeting MTHFD1L
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. CLYBL-Bxb1-LP-v2-TC

   Plasmid

   #194327

   Purpose

   CLYBL targeting construct with the Bxb1 landing pad version 2 cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

   Insert

   loxP-EBFP2-attP-BleoR-lox251
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. plentiCRISPR-v2 Puro EIF2AK4/GCN2_sgRNA

   Plasmid

   #218528

   Purpose

   sgRNA targeting human EIF2AK4/GCN2

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   EIF2AK4 (EIF2AK4 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1-Bxb1-LP-v2-TC

   Plasmid

   #194326

   Purpose

   AAVS1 targeting construct with the Bxb1 landing pad version 2 cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

   Insert

   loxP-EBFP2-attP-BleoR-lox251
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. plentiCRISPR-v2 Puro EIF2AK2/PKR_sgRNA

   Plasmid

   #218527

   Purpose

   sgRNA targeting human EIF2AK2/PKR

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   EIF2AK2 (EIF2AK2 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCRISPR v2-Nr4a1 double gRNAs

   Plasmid

   #162791

   Purpose

   Multiplex Guide RNAs to generate Nr4a1 knockout by CRISPR

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Multiplex Nr4a1 gRNA 1 and 2 (Nr4a1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. plentiCRISPR-v2 Puro EIF2AK3/PERK_sgRNA

   Plasmid

   #218666

   Purpose

   sgRNA targeting human EIF2AK3/PERK

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   EIF2AK3 (EIF2AK3 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiCRISPR v2-sgSLC7A11/xCT-1

   Plasmid

   #161818

   Purpose

   knock out SLC7A11/xCT in mammalian cells

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

   Insert

   SLC7A11 solute carrier family 7 member 11 xCT (SLC7A11 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. plentiCRISPR-v2 Puro EIF2AK1/HRI_sgRNA

    Plasmid

    #218529

    Purpose

    sgRNA targeting human EIF2AK1/HRI

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    EIF2AK1 (EIF2AK1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDY0663 ACTB atgRNA with v2 scaffold

    Plasmid

    #179108

    Purpose

    ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold
    

    Use

    CRISPR

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiCRISPR v2-sgSLC7A11/xCT-2

    Plasmid

    #161819

    Purpose

    knock out SLC7A11/xCT in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

    Insert

    solute carrier family 7 member 11 (SLC7A11 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. 791 p3E-2A-pCS2MCSpA v2

    Plasmid

    #195973

    Purpose

    Gateway p3E 2A fluorophore

    Depositor

    Christian Mosimann, Kristen Kwan

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    2A-multiple cloning site
    

    Use

    Other

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC57kan-T7-gRNA-U6 V2

    Plasmid

    #115520

    Purpose

    To construct multiple sgRNA expression vector for CRISPR

    Depositor

    Xingxu Huang

    Insert

    sgRNA scaffolf-U6 promoter
    

    Use

    CRISPR

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. CROP-seq-puro-v2-no-scaffold

    Plasmid

    #228521

    Purpose

    CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences

    Depositor

    Jellert Gaublomme

    Article

    Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

    Inserts

    Use

    Lentiviral

    Available Since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. CROP-seq-mTurquoise2-v2-no-scaffold

    Plasmid

    #228522

    Purpose

    CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using mTurquoise2 as selection marker

    Depositor

    Jellert Gaublomme

    Article

    Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

    Insert

    mTurquoise2
    

    Use

    Lentiviral

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-mTagBFP2-T2A-iCre (v2)

    Plasmid

    #125822

    Purpose

    Monomeric TagBFP2 and iCre recombinase (version 2)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCre
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. p8092 LentiCRISPR v2 sgNT-1

    Plasmid

    #163315

    Purpose

    Expresses Cas9 and a non-targeting control guide RNA

    Depositor

    Elizabeth White

    Article

    Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

    Insert

    sgNT-1
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. LLP185 pLVP-dCas9-DNMT3a V2

    Plasmid

    #100936

    Purpose

    dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    dCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and 3xTy1

    Expression

    Mammalian

    Mutation

    D10A, H840A for dCas9

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiCRISPR v2 puro - CRBN (#1)

    Plasmid

    #166240

    Purpose

    sgRNA (#1) to generate a knockout of human CRBN by targeting the middle region of Exon1

    Depositor

    Günter Schneider

    Article

    Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

    Insert

    CRBN (CRBN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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