We narrowed to 1,332 results for: pET28a

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pET28a_T7-ARG1

Plasmid

#106473

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli

Depositor

Mikhail Shapiro

Article

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG1

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-Vx3(A7)

Plasmid

#35526

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

Vx3(A7)

Tags

His6

Expression

Bacterial

Mutation

Changed G2 to P

Promoter

T7

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSPdH4-6

Plasmid

#199766

Purpose

Expresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSPdH46

Tags

His6-Tag

Expression

Bacterial

Mutation

internal His-Tag

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-gp32-H6

Plasmid

#163912

Purpose

Expresses T4 gp32 for bacterial expression and affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

gp32

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-uvsX-H6

Plasmid

#163913

Purpose

Expresses uvsX for bacterial expression and affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsX

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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NylCA-TS_pET28a

Plasmid

#215419

Purpose

Expression construct for NylCA-TS gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCA-TS

Expression

Bacterial

Mutation

S111G/A137L/E263Q

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_T7-ARG2

Plasmid

#106474

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coli

Depositor

Mikhail Shapiro

Article

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a Gx-mNG-NL

Plasmid

#169106

Purpose

Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

Depositor

Maarten Merkx

Article

Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

Insert

protein G - mNeonGreen - NanoLuc

Tags

his-tag and strep-tag

Expression

Bacterial

Mutation

Ala 24 stop

Promoter

T7

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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NylCp2-TS_pET28a

Plasmid

#215421

Purpose

Expression construct for NylCp2-TS gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCp2-TS

Expression

Bacterial

Mutation

D122G/H130Y/D36A/E263Q

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-Rx3(A7)

Plasmid

#35525

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

His6-Rx3(A7)

Tags

His6

Expression

Bacterial

Mutation

Changed G2 to P

Promoter

T7

Available Since

April 5, 2012

Availability

Academic Institutions and Nonprofits only

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pET28a(+)- PC-MjMAT

Plasmid

#179372

Purpose

PC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.

Depositor

Andrea Rentmeister

Article

Peters et al Chembiochem. 2021 Oct 4. doi: 10.1002/cb

Insert

PC-MjMAT

Tags

6xHis

Expression

Bacterial

Mutation

L147A, I351A

Promoter

T7

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-Cut-P(11)4

Plasmid

#185785

Purpose

Encodes enzyme cutinase linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

Cut-P(11)4

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-36γ

Plasmid

#214345

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL36G (IL36G Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Human _TFAM_NoMTS_Dimer_pET28a+

Plasmid

#60013

Purpose

Expresses TFAM No MTS dimerization mutant in bacterial cells

Depositor

David Chan

Article

Ngo et al Nat Commun. 2014;5:3077. doi: 10.1038/nc

Insert

Human TFAM No MTS (TFAM Human)

Tags

6xHis

Expression

Bacterial

Mutation

Missing 1st 42 aas (Mitochondrial targeting seque…

Promoter

T7

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-clbH-A1

Plasmid

#114149

Purpose

Expresses ClbH-A1

Depositor

Emily Balskus

Article

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

clbH-A1

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Mutation

Contains only the A1 domain of ClbH

Available Since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-Beclin1 CC

Plasmid

#24403

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains the CC domain (aa 151-242)

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only
pET28a-R4-uAb

Plasmid

#101801

Purpose

Bacterial cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

Depositor

Matthew DeLisa

Article

Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

Insert

R4-uAb

Tags

6xHis and Flag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-36β

Plasmid

#214344

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL36B (IL36B Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-36α

Plasmid

#214343

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL36A (IL36A Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1deltaH4/2

Plasmid

#71712

Purpose

Membrane scaffold protein (MSP), half of helix 4 truncated

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis and TEV site

Expression

Bacterial

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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