163,740 results

1. pGL3 Basic Vector

   Plasmid

   #212936

   Purpose

   Firefly luciferase vector for investigating regions controlling transcription

   Depositor

   Debrya Groskreutz

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available Since

   Jan. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCMV-PE7-P2A-BSD

   Plasmid

   #224122

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7-P2A-BSD
   

   Use

   Tags

   P2A-BSD, SV40 bpNLS, and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. ER-GCaMP6-150

   Plasmid

   #86918

   Purpose

   Fluorescent reporter for ER calcium signaling

   Depositor

   Timothy Ryan

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   ER-GCaMP6-150
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

   Promoter

   CAMKII

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tet-pLKO-puro-Scrambled

   Plasmid

   #47541

   Purpose

   Tet inducible scrambled shRNA.

   Depositor

   Charles Rudin

   Article

   Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

   Insert

   Scrambled shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available Since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1-blast

   Plasmid

   #26655

   Purpose

   3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses blasticidin for selection.

   Depositor

   Keith Mostov

   Article

   Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG416GAL-ccdB

   Plasmid

   #14147

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. MLM3636

   Plasmid

   #43860

   Purpose

   guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter

   Depositor

   Keith Joung

   Insert

   None
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX6P1-mCherry-Nanobody

   Plasmid

   #70696

   Purpose

   Expresses GST-tagged anti-mCherry Nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

   Insert

   mCherry-Nanobody
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICH47742::p35S-ePEmax3::tEURb7

   Plasmid

   #227692

   Purpose

   Golden Gate level 1, position 2, ePEmax3 expression cassette.

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Insert

   ePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available Since

   Nov. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRIS-PITChv2-Puro-dTAG (BRD4)

    Plasmid

    #91793

    Purpose

    PITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

    Depositor

    James Bradner, Behnam Nabet

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    Puro-P2A-2xHA-FKBP_F36V
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promotorless

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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