161,873 results

1. pEThT-CAHS3

   Plasmid

   #90028

   Purpose

   To express His-tagged CAHS3 in bacteria

   Depositor

   Takekazu Kunieda

   Article

   Yamaguchi et al PLoS One. 2012;7(8):e44209.

   Insert

   CAHS3
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-ec_bPAC

   Plasmid

   #28135

   Purpose

   Expression of photoactivated cyclase bPAC of Beggiatoa sp. optimized for E. coli

   Depositor

   Peter Hegemann

   Article

   Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

   Insert

   E.coli adapted photoactivated adenylyl cyclase
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. LV_EF1a_KRAS(G12V)-P2A-Hygro_Barcode

   Plasmid

   #170237

   Purpose

   Barcoded lentiviral vector to express KRAS (G12V) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   KRAS (KRAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   G12V

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. HCTR1-Tango

   Plasmid

   #66398

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   HCTR1 (HCRTR1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pLyn-FRB-mCherry-nmd3-BSD

   Plasmid

   #85796

   Purpose

   Expression of PPM mislocaliser in P. falciparum

   Depositor

   Tobias Spielmann

   Article

   Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   PPM Mislocaliser
   

   Use

   P. falciparum expression

   Tags

   FRB*, mCherry

   Expression

   Mutation

   Promoter

   Pfnmd3

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. LPUtopia-7

   Plasmid

   #199212

   Purpose

   Landing Pad Utopia_v7 donor plasmid for AAVS1 site-specific integration in human cells. Use NeoR and eGFP as positive selection marker for AAVS1-targeted insertion and RFP as negative selection marker

   Depositor

   Gabor Balazsi

   Article

   Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

   Inserts

   aminoglycoside phosphotransferase,
   enhanced Green Fluorescence Protein
   Red Fluorescence Protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and thymidine kinase promoter

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TRE-STOP EF1a-rtTA3

   Plasmid

   #195882

   Purpose

   control construct of TRE-VEGFA EF1a-rtTA3

   Depositor

   Elaine Fuchs

   Article

   Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

   Insert

   VEGFA (Vegfa Synthetic, Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBMN ltDHFR(DD)-YFP

   Plasmid

   #47076

   Purpose

   ecDHFR based destable domain function at low temperature for regulating protein stability in Caenorhabditis elegans

   Depositor

   Thomas Wandless

   Article

   Cho et al PLoS One. 2013 Aug 22;8(8):e72393. doi:

   Insert

   ltDHFR(DD)
   

   Use

   Retroviral

   Tags

   HA and YFP

   Expression

   Mutation

   amino acids mutations: R12H, N23S, G67S, V78A, E1…

   Promoter

   LTR

   Available since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSWAP_Entry_HA

   Plasmid

   #184866

   Purpose

   pSwap plasmid part with HA homology arms

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   lacZalpha,ccdB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pSWAP_Entry_HB

    Plasmid

    #184868

    Purpose

    pSwap plasmid part with HB homology arms

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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