We narrowed to 6,905 results for: crispr cas9 plasmids

1. pACRISPR

   Plasmid

   #113348

   Purpose

   A sgRNA expression plasmid for genome editing in Pseudomonas aeruginosa

   Depositor

   Quanjiang Ji

   Article

   Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-hSyn-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

   Plasmid

   #177345

   Purpose

   AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from Synapsin promoter

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   dead hSaCas9
   

   Use

   AAV and CRISPR

   Tags

   3x GCN4 peptide, 3xHA, and NLS

   Expression

   Mammalian

   Mutation

   D10A, N580A

   Promoter

   Synapsin

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-TRE-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

   Plasmid

   #177344

   Purpose

   AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from doxycycine-inducible promoter

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   dead hSaCas9
   

   Use

   AAV and CRISPR

   Tags

   3x GCN4 peptide, 3xHA, and NLS

   Expression

   Mammalian

   Mutation

   D10A, N580A

   Promoter

   Tetracycline-dependent promoters

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-CMV-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

   Plasmid

   #177342

   Purpose

   AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   dead hSaCas9
   

   Use

   AAV and CRISPR

   Tags

   3x GCN4 peptide, 3xHA, and NLS

   Expression

   Mammalian

   Mutation

   D10A, N580A

   Promoter

   CMV

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBK1295-AAV-EFSNC-dCjCas9-HP1a(72-177)

   Plasmid

   #223140

   Purpose

   Expression of truncated HP1a with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   HP1a (CBX5 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 72-177

   Promoter

   EF1a

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBK1305-AAV-EFSNC-dSaCas9-HP1a(72-177)

   Plasmid

   #223150

   Purpose

   Expression of truncated HP1a with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   HP1a (CBX5 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 72-177

   Promoter

   EF1a

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-eCas9-T2A-EGFP-U6-gRNA targeting Nrl-no ITR and f1 ori

   Plasmid

   #234737

   Purpose

   All-in-one CRISPR/Cas9 vector encodes high-fidelity eSpCas9 and a gRNA targeting Nrl, efficiently reprogramming rod precursors into cone-like cells in the mouse retina.

   Depositor

   Kirill Martemyanov

   Article

   Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

   Insert

   Nrl (Nrl Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. 874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

   Plasmid

   #159672

   Purpose

   Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   βTub-NLS-hSpCas9-T2A-GFP
   

   Use

   Tags

   T2A-GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. 874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

   Plasmid

   #159671

   Purpose

   Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   ExuL-NLS-hSpCas9-T2A-GFP
   

   Use

   Tags

   T2A-GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSEVA231-CRISPR

    Plasmid

    #138712

    Purpose

    Plasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR Array

    Depositor

    Víctor de Lorenzo

    Article

    Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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