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Plasmid#121464Purpose2µm HIS3 CNB1mutEF2DepositorInsertCNB1mutEF2 (CNB1 Budding Yeast)
UseYeast high copy 2 micron vectorTagsExpressionMutationChanged aspartic acid 66 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK508
Plasmid#121463Purpose2µm HIS3 CNB1mutEF1DepositorInsertCNB1mutEF1 (CNB1 Budding Yeast)
UseYeast high copy 2 micron vectorTagsExpressionMutationChanged aspartic acid 34 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK516
Plasmid#121473PurposeCEN LEU2 CNB1mutEF4DepositorInsertCNB1mutEF4 (CNB1 Budding Yeast)
UseYeast cen plasmidTagsExpressionMutationChanged aspartic acid 144 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK506
Plasmid#121461PurposeCEN HIS3 CNB1mutEF4DepositorInsertCNB1mutEF4 (CNB1 Budding Yeast)
UseYeast cen plasmidTagsExpressionMutationChanged aspartic acid 144 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK505
Plasmid#121460PurposeCEN HIS3 CNB1mutEF3DepositorInsertCNB1mutEF2 (CNB1 Budding Yeast)
UseYeast cen plasmidTagsExpressionMutationChanged aspartic acid 103 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK504
Plasmid#121459PurposeCEN HIS3 CNB1mutEF2DepositorInsertCNB1mutEF2 (CNB1 Budding Yeast)
UseYeast cen plasmidTagsExpressionMutationChanged aspartic acid 66 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK503
Plasmid#121458PurposeCEN HIS3 CNB1mutEF1DepositorInsertCNB1mutEF1 (CNB1 Budding Yeast)
UseYeast cen plasmidTagsExpressionMutationChanged aspartic acid 34 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK518
Plasmid#121475PurposeCEN TRP1 CNA1ΔAIDDepositorInsertCNA1deltaAID
UseYeast cen vectorTagsExpressionMutationTruncated after amino acid 508PromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK519
Plasmid#121476PurposeCEN TRP1 CNA1ΔCBDDepositorInsertCNA1deltaCBD
UseYeast cen vectorTagsExpressionMutationTruncated after amino acid 453PromoterEndogenous genomic promoter regionAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pRRG217-soxB
Plasmid#99086PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea soxB (also known as soxB1-1)DepositorInsertsoxB (also known as soxB1-1) in situ probe
UseT/a cloning vector for dsrna generation and the g…TagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRRG325-Unc-9
Plasmid#99084PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea Unc-9 (also known as h.26.12f)DepositorInsertUnc-9 (also known as h.26.12f) in situ probe
UseT/a cloning vector for dsrna generation and the g…TagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRRG246-MCP
Plasmid#99083PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea MCP (also known as h.45.8g)DepositorInsertMCP (also known as h.45.8g) in situ probe
UseT/a cloning vector for dsrna generation and the g…TagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRRG208-ActR1
Plasmid#99077PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea ActR1 (activin receptor 1)DepositorInsertActR1 (activin receptor 1) in situ probe
UseT/a cloning vector for dsrna generation and the g…TagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRRG128-activin
Plasmid#99075PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea Activin (also known as activin 2)DepositorInsertActivin (also known as activin 2) in situ probe
UseT/a cloning vector for dsrna generation and the g…TagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA27_no stop
Plasmid#83860PurposeGateway donor vector for IAA27 with no stop codonDepositorInsertIAA27 (PAP2 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationno stop codonPromoterAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA8_no stop
Plasmid#83874PurposeGateway donor vector for IAA8 with no stop codonDepositorInsertindole-3-acetic acid inducible 8 (IAA8 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationno stop codonPromoterAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA5_no stop
Plasmid#83868PurposeGateway donor vector for IAA5 with no stop codonDepositorInsertIAA5 (IAA5 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationno stop codonPromoterAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA3_no stop
Plasmid#83863PurposeGateway donor vector for IAA3 with no stop codonDepositorInsertIAA3 (SHY2 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationno stop codonPromoterAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA10_no stop
Plasmid#83848PurposeGateway donor vector for IAA10 with no stop codonDepositorInsertindoleacetic acid-induced protein 10 (IAA10 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationno stop codonPromoterAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA4_no stop
Plasmid#83866PurposeGateway donor vector for IAA4 with no stop codonDepositorInsertIAA4 (ATAUX2-11 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationno stop codonPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA28_no stop
Plasmid#83861PurposeGateway donor vector for IAA28 with no stop codonDepositorInsertNP_568478.1 (IAA28 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationno stop codonPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA15_no stop
Plasmid#83853PurposeGateway donor vector for IAA15 with no stop codonDepositorInsertindole-3-acetic acid inducible 15 (IAA15 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationno stop codonPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBW301ParsR-Amp30T
Plasmid#78653PurposepSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-30hrpS-t-hrpL-30gfp-tDepositorInsertaraC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
mSTIM1_1-250-CFP
Plasmid#69552PurposeExpresses a STIM1_1-250 fragment mammalian cellsDepositorInsertmSTIM1_1-250
UseTagsCyan Fluorescent Protein (CFP)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-Cx43-FS
Plasmid#65440PurposeCloning, T7 RNA expressionDepositorInsertCx43 (GJA1 Human)
UseT7 in vitro rna productionTagsExpressionBacterialMutationFrameshift (FS) mutation 35delAPromoterT7Available sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL 1410
Plasmid#53011PurposepLtetO-1:DsrA, pCon:ompC::T7noRBS(900bps), pLlacO-1:rpoS::gfpDepositorInsertsDsrA sRNA
ompC
rpoS
UseSynthetic BiologyTagsT7noRBS (900bp) and gfpExpressionBacterialMutationcontains −150 to +30 bp relative to start codon a…PromoterpCon, pLlacO-1, and pLtetO-1Available sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB693
Plasmid#60145PurposeDisintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertYeLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1853
Plasmid#53040PurposePLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrADepositorInsertsGFP
tetR
csrA
UseSynthetic BiologyTagsLVA, RBS(st2), RBS(st3), and glgC leaderExpressionBacterialMutationPromoterpConNoHind and pLlac0-1Available sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)
Plasmid#52898PurposeYpdI* is fused to the IFP-F[2] by (GGGGS) X 2. Ypd1*/Skn7t: no signal, Ypd1*/Ssk1t: weak signal. Internal ClaI site in Ypd*I. Plasmid confers ampicillin resistance to DH5α.DepositorInsertYpd*1-L-IFP-F[2]
UseTagsL-IFP-F[2]ExpressionYeastMutationChanged Tryptophane 80 to AlaninePromoterGAL1Available sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only