We narrowed to 4,339 results for: 187
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Plasmid#221648PurposeConstitutive ectopic expression of human PTPN14 C1121S mutantDepositorInsertPTPN14 C1121S (PTPN14 Human)
UseLentiviralTagsV5ExpressionMutationC1121S mutation at the putative catalytic sitePromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8391 LentiCRISPRv2 Neo sgPTPN14-1
Plasmid#221651PurposeExpression of sgRNA targeting the locus of human PTPN14DepositorInsertsgPTPN14-1 (PTPN14 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8392 LentiCRISPRv2 Neo sgPTPN14-3
Plasmid#221652PurposeExpression of sgRNA targeting the locus of human PTPN14DepositorInsertsgPTPN14-3 (PTPN14 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AP-SpyCatcher
Plasmid#223636PurposeProtein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsHis10, AP fragment of split APEX2 tagExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyCatcher -AVI
Plasmid#223635PurposeProtein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsAVI tag and His6ExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY50
Plasmid#223443Purposembr-1 fluorescent neural reporter driving nuclear mNeptune2.5 and mTagBF2 expression (refer to NeuroPAL paper for expression)DepositorInsertmbr-1 (mbr-1 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-λN-HA-NOT1 isoform C (JM155)
Plasmid#206449PurposeNot1 expression in Schneider cells for imagingDepositorInsertNot1 (Not1 Fly)
UseTagslambda N HAExpressionInsectMutationPromoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-mEGFP-NOT1 isoform C (HK53)
Plasmid#206440PurposeNOT1 expression in Schneider cells for imagingDepositorInsertNOT1 (Not1 Fly)
UseTagsmEGFPExpressionInsectMutationPromoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-mEGFP-NOT2 isoform A (HK54)
Plasmid#206441PurposeNOT1 expression in Schneider cells for imagingDepositorInsertNOT1 (Not1 Fly)
UseTagsmEGFPExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-RIPLET ΔPRY/SPRY
Plasmid#218516PurposeRIPLET lacking C-terminal PRY/SPRY domain in pcDNA3.1DepositorInsertRIPLET delta PRY/SPRY domain (RNF135 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRD2 CTF-FLAG
Plasmid#217688PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRD2 (ADGRD2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-636PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRD2 CTFdeltaTA-FLAG
Plasmid#217721PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRD2 (ADGRD2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-642PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC160
Plasmid#202248PurposeLevel 2 for strain-specific barcode DNA tag with bc187 through Tn7 bacterial insertion, Gentamicin selectable marker and AmCyan1 fluorescent markerDepositorInsertbc187
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
hLig4 guide2 in pX458
Plasmid#211536PurposesgRNA-2 against Lig4DepositorInsertsgRNA hLig4 (LIG4 Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoteru6Available sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
hLig4 guide1 in pX458
Plasmid#211535PurposesgRNA-1 against Lig4DepositorInsertsgRNA hLig4 (LIG4 Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoteru6Available sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
hPRL2_1-163
Plasmid#209794PurposeBacterial expression of human PRL-2 without farnesylation motifDepositorInsertPRL-2 (PTP4A2 Human)
UseTags6xHisExpressionBacterialMutationaa 1-163 onlyPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
acvr1l-VAAA-HA
Plasmid#201905Purposein vitro transcription of RNA encoding HA-tagged Acvr1l that contains 4 amino acid substitutions: T186V, S187A, S189A, S191ADepositorInsertacvr1l (acvr1l Zebrafish)
UseTagsHAExpressionMutationT186V, S187A, S189A, S191APromoterSP6Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3B DD 593-747
Plasmid#193932PurposeMammalian expression of GFP-tagged Kif 3B DD 593-747DepositorInsertKif 3B (Kif3b Mouse)
UseTagsGFPExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-DmNot1_1710-2505_R
Plasmid#147440PurposeBacterial Expression of DmNot1_1710-2505DepositorInsertDmNot1_1710-2505 (Not1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1-dsRNAres_N
Plasmid#147029PurposeInsect Expression of DmNot1-dsRNAresDepositorInsertDmNot1-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only