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pCDNA3-Flag-hDcp2

Plasmid

#72213

Purpose

mammalian expression of Flag-tagged human Dcp2

Depositor

Megerditch Kiledjian

Article

Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

Insert

Dcp2 (DCP2 Human)

Tags

Flag

Expression

Mammalian

Mutation

Q170R (please see depositor's comment below)

Promoter

CMV

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-hTRAF3 333-C

Plasmid

#44035

Depositor

Shao-Cong Sun

Article

Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

Insert

TNF receptor-associated factor 3 (TRAF3 Human)

Tags

1xHA

Expression

Mammalian

Mutation

Contains amino acids 334-568; S393G and K492N

Available Since

April 4, 2013

Availability

Academic Institutions and Nonprofits only
PKC-delta-IRES-hrGFP

Plasmid

#73414

Purpose

Mammalian expression of mouse PKC-delta and hrGFP

Depositor

Raymond Swanson

Article

Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

Insert

PKC-delta (Prkcd Mouse)

Tags

3xFLAG and IRES-hrGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only
mEos2-Alpha-5-Integrin-12

Plasmid

#57344

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Alpha-5 Integrin (ITGA5 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCellFree_G03 ISG15

Plasmid

#67054

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

ISG15 (ISG15 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pGL2 p15 4xSBR1

Plasmid

#15706

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

p15 4xSBR1 (CDKN2B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pGL2 p15 4xSBR2

Plasmid

#15709

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

p15 4xSBR2 (CDKN2B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
isoform PKC Alpha (JCE599)

Plasmid

#89779

Purpose

yeast expression of mouse PKC Alpha with yeast PKC1 promoter and ADH1 terminator

Depositor

Juan Carlos Igual

Article

Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

Insert

PKC Alpha (Prkca Mouse)

Expression

Mammalian

Promoter

yeast PKC1 promoter (-600 to -1)

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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aL-mEos3.2

Plasmid

#113154

Purpose

constrained LFA-1-mEos3.2 fusion

Depositor

Timothy Springer

Article

Moore et al Cell Rep. 2018 Feb 13;22(7):1903-1912. d

Insert

full-length human aL with mEOS3.2 in betapropeller (ITGAL Human)

Tags

mEOS3.2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLTR PKC delta

Plasmid

#8419

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C delta (Prkcd Mouse)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
boP58-pBabe.puro(notag)

Plasmid

#21885

Depositor

David Ron

Article

Oyadomari et al Cell. 2006 Aug 25. 126(4):727-39.

Insert

P58 (DNAJC3 )

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pECFP-N2-COL1A1

Plasmid

#66603

Purpose

Expresses procollagen 1 alpha 1 as a CFP fusion

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

Insert

COL1A1 (COL1A1 Human)

Expression

Mammalian

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-HA-hTRAF3 88-C

Plasmid

#44034

Depositor

Shao-Cong Sun

Article

Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

Insert

TNF receptor-associated factor 3 (TRAF3 Human)

Tags

1xHA

Expression

Mammalian

Mutation

Contains amino acids 89-568; V274D and S393G

Available Since

March 27, 2013

Availability

Academic Institutions and Nonprofits only
2xFKBP-myc

Plasmid

#20212

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

2xFKBP (FKBP1A Human)

Tags

myc

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pTRIPZ-DoxOn-shCUX1-5150

Plasmid

#90469

Purpose

Lentiviral vector expressing a doxycycline-inducible shRNA against human CUX1 sequence starting at 5150 of M74099

Depositor

Alain Nepveu

Article

Ramdzan et al PLoS Biol. 2014 Mar 11;12(3):e1001807. d

Insert

TGCTGTTGACAGTGAGCGTCAGAGCGATAATACACTATTATAGTGAAGCC

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Ub-UBB+1

Plasmid

#23967

Depositor

Nico Dantuma

Article

Lindsten et al J Cell Biol. 2002 Apr 29. 157(3):417-27.

Insert

Ubiquitin B +1 transcript (UBB Human)

Expression

Mammalian

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

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Pmyo3::tag:sl1(G16C)

Plasmid

#79003

Purpose

Expression of exogenous SL1 in C. elegans muscle

Depositor

Xiao Liu, Ge Zhan

Article

Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

Insert

sls-1.2 (sls-1.2 Nematode)

Tags

illumina-adapter

Expression

Worm

Mutation

G16C

Promoter

myo-3

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLTR PKC alpha

Plasmid

#8416

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C alpha (Prkca Mouse)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
Vh-HA-2xFKBP

Plasmid

#20221

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

2xFKBP (FKBP1A Human)

Tags

HA and Vh

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pBacPAK8-ElonginC

Plasmid

#29505

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Elongin C (ELOC Human)

Expression

Insect

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only