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pMDJ80 - pENTR[4-1] - Promoter - Psmu-2 (no_start)

Plasmid

#159887

Purpose

Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[4-1] - Promoter - Psmu-2 (no_start)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMNK56 - piRNAi 21ur-1224 - him-8

Plasmid

#159819

Purpose

piRNA-induced RNA interference (piRNAi) to induce males in transgenic animals

Depositor

Christian Froekjaer-Jensen

Publication

Priyadarshini et al Nat Methods. 2022 Feb;19(2):187-194. doi

Insert

him-8 piRNAi 21ur-1224

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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TC393

Plasmid

#122625

Purpose

Pser-2::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

Depositor

Tian Chi

Publication

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

Expression

Worm

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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BSP188

Plasmid

#110917

Purpose

For cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator cloned

Depositor

Alexander Mendenhall

Publication

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

unc54 Terminator

Use

Synthetic Biology; Yeast homologous recombination…

Expression

Worm

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pJJR50

Plasmid

#75026

Purpose

U6 promoter driven flipped + extended sgRNA expression vector

Depositor

Mike Boxem

Publication

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

guide RNA, flipped and extended version

Use

CRISPR

Promoter

R07E5.16 (U6)

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pHAGE-CMV-GFP-W

Plasmid

#196046

Purpose

lentiviral expression of the green fluorescent protein driven by cytomegalovirus virus promoter

Depositor

Darrell Kotton

Publication

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Type

Empty backbone

Use

Lentiviral

Tags

eGFP

Promoter

CMV

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_PCNA_PCNA

Plasmid

#110022

Purpose

Protein expression and purification of PCNA_PCNA

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PCNA_PCNA (PCNA Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_PFN1_Profilin

Plasmid

#110028

Purpose

Protein expression and purification of PFN1_Profilin

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PFN1_Profilin (PFN1 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBFv-UAS3-GFP1-10

Plasmid

#138398

Purpose

Expresses split GFP fragment (GFP1-10)

Depositor

Shu Kondo

Publication

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

GFP1-10

Expression

Insect

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCZ829

Plasmid

#110137

Purpose

Epidermal Mitochondrial-tagged Florescent Calcium Sensor

Depositor

Andrew Chisholm

Publication

Xu et al Dev Cell. 2014 Oct 13;31(1):48-60. doi:

Insert

mito-GCaMP5

Tags

mito::GCaMP5

Expression

Worm

Promoter

Pcol-19

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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R777-E330 Hs.SPRY2-nostop

Plasmid

#70614

Purpose

Gateway ORF clone of human SPRY2 [NM_005842.2] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

SPRY2 (SPRY2 Human)

Use

Gateway Cloning

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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sgRNA with rpr-1 promoter

Plasmid

#48961

Purpose

to drive the sgRNA expression under RNase P non-coding RNA promoter

Depositor

Mario de Bono

Publication

Chen et al Nucleic Acids Res. 2013 Sep 5.

Type

Empty backbone

Use

CRISPR

Expression

Worm

Promoter

rpr-1 promoter

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSAH49 - [PVQp | mScarlet | tbb-2 UTR]

Plasmid

#200325

Purpose

mScarlet BioPart for PVQ neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.

Depositor

Christian Froekjaer-Jensen

Publication

AlHarbi et al MicroPubl Biol. 2023 Jun 22;2023:10.1791

Insert

[PVQp | wrmscarlet | tbb-2 UTR]

Expression

Worm

Promoter

WBGene00003755, nlp-17

Available since

June 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSEM236 - pEXP[Pmlc-2 | histamine | rpl-3 UTR]

Plasmid

#159797

Purpose

Histamine based negative selection marker to paralyze animals with arrays

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

Insert

pEXP[Pmlc-2 | histamine | rpl-3 UTR]

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pNM3734

Plasmid

#154118

Purpose

mec-4 promoter driving GAL4-QFAD in an RCME vector

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p::Gal4SK::QFAD::tbb-2 '3

Use

Rmce

Expression

Worm

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGM59

Plasmid

#74785

Purpose

Expresses 3XFLAG::GR LBD::DAF-16A from the hsp16.48 promoter

Depositor

Jordan Ward, Keith Yamamoto

Publication

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Insert

3X FLAG::GR LBD::DAF-16A (daf-16, NR3C1 Human, Nematode, Synthetic)

Expression

Worm

Promoter

hsp16.48

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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SREC 2: abu-1::abu-1::gfp

Plasmid

#21825

Depositor

David Ron

Publication

Urano et al J Cell Biol. 2002 Aug 19. 158(4):639-46.

Insert

abu-1 promoter and coding region (abu-1 Nematode)

Tags

GFP

Expression

Worm

Available since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pMS59

Plasmid

#215680

Purpose

Cas9 + guide plasmid targeting ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Publication

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG

Use

CRISPR

Expression

Worm

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPD95.77-wVenus

Plasmid

#37465

Depositor

Hitoshi Sawa

Publication

Yamamoto et al PLoS Genet. 2011 Oct;7(10):e1002308. Epu

Type

Empty backbone

Expression

Worm

Promoter

none

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMLS234

Plasmid

#73682

Purpose

SapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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