We narrowed to 14,886 results for: pET

1. pETG20a-Salhel-HMG

   Plasmid

   #231537

   Purpose

   Expresses the HMG of Salhel Sox-I, for protein purification

   Depositor

   Ralf Jauch

   Article

   Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

   Insert

   Sox-I HMG
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. HemH-R212A_pET-14b

   Plasmid

   #233262

   Purpose

   Overexpress his-tag HemH protein with a site directed mutagenesis, arginine at position 212 was substituted with alanine (R121A)

   Depositor

   Mohan Babu, Andrew Emili

   Article

   Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

   Insert

   HemH (hemH )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Arginine was replaced with Alanine at position 21…

   Promoter

   T7 promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-Impact-T4gp41

   Plasmid

   #228207

   Purpose

   Expression in BL21(DE3) of T4 helicase

   Depositor

   Stephen Benkovic

   Insert

   41 (41 Escherichia phage T4)
   

   Use

   Tags

   Sce VMA intein-chitin binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-Impact-T4gp59

   Plasmid

   #228206

   Purpose

   Expression in BL21(DE3) of WT T4 helicase loader

   Depositor

   Stephen Benkovic

   Insert

   59 (59 Escherichia phage T4)
   

   Use

   Tags

   Sce VMA intein-chitin binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28-NFHΔ462-638

   Plasmid

   #232061

   Purpose

   Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638

   Depositor

   Sanjay Kumar

   Article

   Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

   Insert

   Neurofilament-Heavy tail domain lacking residues 462-638
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   added N-terminal tryptophan and tyrosine, and cys…

   Promoter

   Available since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET14-H2Kb-ST3

   Plasmid

   #223221

   Purpose

   Expression of mouse MHC H2Kb heavy chain fused to Spytag003 under T7 promoter for expression in bacteria

   Depositor

   Omer Dushek

   Article

   Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

   Insert

   Expression of soluble mouse MHC H2KB heavy chain (HLA-A Mouse)
   

   Use

   Tags

   Spytag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-BoxB-petracrRNA

   Plasmid

   #207625

   Purpose

   Expresses BoxB-petracrRNA in mammalian cells (with a human-U6 promoter)

   Depositor

   Jay Shendure

   Article

   Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

   Insert

   BoxB-petracrRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28_Strep-Psc-pScaC

   Plasmid

   #228834

   Purpose

   E. coli expression of the ScaC passenger domain

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   ScaC
   

   Use

   Tags

   Strep

   Expression

   Bacterial

   Mutation

   aa 33-223

   Promoter

   Available since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-OuIAD(E502Q)

   Plasmid

   #226973

   Purpose

   Expressing Olsenella uli indoleacetate decarboxylase (IAD) E502Q variant in E. coli

   Depositor

   Emily Balskus

   Article

   Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

   Insert

   OuIAD(E502Q)
   

   Use

   Tags

   His6 (on backbone)

   Expression

   Bacterial

   Mutation

   E502Q

   Promoter

   Available since

   Jan. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21-pelB-GST-7

    Plasmid

    #228989

    Purpose

    For bacterial expression of anti-GST nanobody GST-7, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    GST-7
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21-pelB-LaG94-9

    Plasmid

    #228968

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-9
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21-pelB-LaG94-10

    Plasmid

    #228969

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-10, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-10
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21-pelB-LaG94-12

    Plasmid

    #228970

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-12, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-12
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21-pelB-LaG94-3

    Plasmid

    #228963

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-3, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-3
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21-pelB-LaTdT-38

    Plasmid

    #228979

    Purpose

    For bacterial expression of anti-tdTomato nanobody LaTdT-38, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaTdT-38
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21-pelB-LaG94-8

    Plasmid

    #228967

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-8, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-8
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21-pelB-LaG94-6

    Plasmid

    #228966

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-6, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-6
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21-pelB-LaG94-18

    Plasmid

    #228972

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-18, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-18
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21-pelB-LaTdT-2

    Plasmid

    #228975

    Purpose

    For bacterial expression of anti-tdTomato nanobody LaTdT-2, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaTdT-2
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21-pelB-LaGIgG-4

    Plasmid

    #228998

    Purpose

    For bacterial expression of anti-mouse IgG nanobody LaMIgG-4, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaGIgG-4
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge