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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 14,580 results for: pET

Showing: 2381 - 2400 of 14580 results

  11. pET29a-DDX53-Q249A

    Plasmid
    #222216
    Purpose
    Express DDX53 mutant Q249A protein in bacteria
    Depositor
    Yuliang Wu
    Insert
    DDX53_Q249A (DDX53 Human)
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    K272A
    Promoter
    T7
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222216-plasmid-map-sequence-id-441427

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  12. pET29a-DDX53-K272A

    Plasmid
    #222215
    Purpose
    Express DDX53 mutant K272A protein in bacteria
    Depositor
    Yuliang Wu
    Insert
    DDX53_K272A (DDX53 Human)
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    K272A
    Promoter
    T7
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222215-plasmid-map-sequence-id-441662

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  13. pET29a-DDX53-E377Q

    Plasmid
    #222217
    Purpose
    Express DDX53 mutant E377Q protein in bacteria
    Depositor
    Yuliang Wu
    Insert
    DDX53_E377Q (DDX53 Human)
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    E377Q
    Promoter
    T7
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222217-plasmid-map-sequence-id-441653

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  14. pET41-FeoB-C675S

    Plasmid
    #216756
    Purpose
    Investigation of the potential interaction of the FeoB Cys675 residue with ferrous iron
    Depositor
    Rietie Venter
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Amino acid C675 is substituted with S.
    Promoter
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    216756-plasmid-map-sequence-id-434497

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  15. pET41-FeoB-C429S

    Plasmid
    #216755
    Purpose
    Investigation of the potential interaction of the FeoB Cys429 residue with ferrous iron
    Depositor
    Rietie Venter
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Amino acid C429 is substituted with S.
    Promoter
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    216755-plasmid-map-sequence-id-434494

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  16. pET41-FeoB-D123N

    Plasmid
    #216754
    Purpose
    Production of a FeoB that is unable to bind GTP for a negative control in GTPases assays
    Depositor
    Rietie Venter
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Amino acid D123 is substituted with N.
    Promoter
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    216754-plasmid-map-sequence-id-434490

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  18. pETSUMO2_Ideonella-bGSDM-WELQ

    Plasmid
    #222098
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Ideonella species with a WELQut protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Ideonella-bGSDM-WELQ ORF
    Use
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222098-plasmid-map-sequence-id-437096

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  19. pETSUMO2_Vitiosangium-bGSDM-thrombin

    Plasmid
    #222099
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Vitiosangium-bGSDM-thrombin ORF
    Use
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222099-plasmid-map-sequence-id-437094

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  20. pETSUMO2_Nostoc-bGSDM-WELQ

    Plasmid
    #222100
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Nostoc-bGSDM-WELQ ORF
    Use
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222100-plasmid-map-sequence-id-437100

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Showing: 2381 - 2400 of 14580 results