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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 7,255 results for: ALP

Showing: 4801 - 4840 of 7255 results

  3. mEos3.2-MCPA2-C-18

    Plasmid
    #57469
    Purpose
    Localization: Actin Capping Protein , Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    MCPA2 (Capza2 Mouse)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57469-plasmid-map-sequence-id-210751

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  4. SOX17-tdTomato-EhB

    Plasmid
    #210467
    Purpose
    Donor plasmid for knock-in tdTomato into the human SOX17 locus
    Depositor
    Kai Wang
    Insert
    SOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived hygromycin-NLS-mTagBFP2 selection cassette (SOX17 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    210467-plasmid-map-sequence-id-419023

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  6. CAPZA1-TwinStrepII

    Plasmid
    #83193
    Purpose
    Expression of tagged protein in mammalian cells
    Depositor
    Peter Barr-Gillespie
    Insert
    CAPZA1 (Capza1 Mouse)
    Use
    Tags
    Twin Strep Tag II
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    83193-plasmid-map-sequence-id-163489

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  8. pIC144

    Plasmid
    #63067
    Purpose
    Retroviral expression of EGFP-TEV-S-tag CENP-K for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    CENP-K, IMAGE clone 3511065 (CENPK Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    63067-plasmid-map-sequence-id-134679

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  13. APPL1-N308D-GFP

    Plasmid
    #102783
    Purpose
    Point mutation in APPL1 that diminishes its interaction with Rab5
    Depositor
    Donna Webb
    Insert
    APPL1 (APPL1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    changed asparagine 308 to aspartate; *(see below)
    Promoter
    CMV
    Available since
    Jan. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    102783-plasmid-map-sequence-id-198005

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  14. BAZ2A

    Plasmid
    #73261
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BAZ2A (BAZ2A Human)
    Use
    Tags
    His-6-SUMO
    Expression
    Bacterial
    Mutation
    pfam00628:PHD 1652-1696
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. pGEM-GluCl_cryst

    Plasmid
    #31487
    Depositor
    Eric Gouaux
    Article
    Hibbs et al Nature. 2011 Jun 2. 474(7349):54-60.
    Insert
    C. elegans glutamate-gated chloride channel alpha, crystallized construct
    Use
    For rna synthesis for expression in x. laevis ooc…
    Tags
    8x-His
    Expression
    Mutation
    Promoter
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only

  16. APPL1-N308D-mCherry

    Plasmid
    #102784
    Purpose
    Point mutation in APPL1 that diminishes its interaction with Rab5
    Depositor
    Donna Webb
    Insert
    APPL1 (APPL1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    changed asparagine 308 to aspartate; *(see below)
    Promoter
    CMV
    Available since
    Jan. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    102784-plasmid-map-sequence-id-197993

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  18. CAPN13

    Plasmid
    #25622
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CAPN13 (CAPN13 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acid residues 515-669
    Promoter
    Available since
    Aug. 31, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. CAPN9 (2P0R)

    Plasmid
    #25138
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    capn09.010.340 (CAPN9 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. mEos Cav2.2 W391A pRK5

    Plasmid
    #206090
    Purpose
    expression of rabbit Cav2.2 calcium channel with a N-terminal mEos tag and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2
    Depositor
    Annette Dolphin
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    mEos
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206090-plasmid-map-sequence-id-415438

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  24. Ef1a-DIO-somAion-citrine

    Plasmid
    #192573
    Purpose
    AAV-vector for optogenetic long-term silencing of Cre-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somAion
    Use
    AAV
    Tags
    Citrine - soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…
    Promoter
    Ef1-alpha
    Available since
    Dec. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    192573-plasmid-map-sequence-id-379368

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  25. R777-E146 Hs.NRAS-nostop

    Plasmid
    #70430
    Purpose
    Gateway ORF clone of human NRAS [NM_002524.4] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    NRAS (NRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70430-plasmid-map-sequence-id-125879

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  27. mEos4a-Cx43-7

    Plasmid
    #57492
    Purpose
    Localization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson, Loren Looger
    Insert
    Cx43 (Gja1 Rat)
    Use
    Tags
    mEos4a
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 4, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57492-plasmid-map-sequence-id-213050

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  28. pQdCas12a.luxR(mut)-empty

    Plasmid
    #236037
    Purpose
    The plasmid pQdCas12a.luxR(mut)-empty expresses the dCas12a endonuclease. Additionally, it contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI, dcas12a, and lacZ-alpha (fragment).
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    quorum sensing promoter
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236037-plasmid-map-sequence-id-469909

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  37. NK803

    Plasmid
    #166057
    Purpose
    Puromycin resistance and LifeAct-mCherry expression for Salpingoeca rosetta
    Depositor
    Nicole King
    Article
    Brunet et al Elife. 2021 Jan 15;10. pii: 61037. doi:
    Inserts
    Pac puromycin resistance gene
    LifeAct-mCherry
    Use
    S. rosetta expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    166057-plasmid-map-sequence-id-330645

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Showing: 4801 - 4840 of 7255 results