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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,387 results for: BLI;

Showing: 4801 - 4840 of 8387 results

  1. SOCS6

    Plasmid
    #38871
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SOCS6 (SOCS6 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    V368D, R463G
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  2. USP37

    Plasmid
    #36898
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP37 (USP37 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    contains amino acid residues 4-125 only
    Promoter
    Available since
    June 21, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. pZC04 (pFA6a-PSc-6xGly-PrtA-His3MX)

    Plasmid
    #44066
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, His3…
    Expression
    Mutation
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44066-plasmid-map-sequence-id-61687

    Enlarge

  4. pHH0103 APBB3 WW domain

    Plasmid
    #104262
    Purpose
    Bacterial expression of WW domain from APBB3
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    APBB3 WW domain (APBB3 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104262-plasmid-map-sequence-id-203722

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  5. pHH0103 APBB2 WW domain

    Plasmid
    #104257
    Purpose
    Bacterial expression of WW domain from APBB2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    APBB2 WW domain (APBB2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    104257-plasmid-map-sequence-id-201510

    Enlarge

  6. pTF263 (pFA6a-TEV-6xGly-12xHis-His3MX)

    Plasmid
    #44069
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    12xHis tag, 6xGly linker, ADH1 transcription term…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44069-plasmid-map-sequence-id-61666

    Enlarge

  7. pTF276 (pFA6a-PSc-6xGly-2xStrep-His3MX)

    Plasmid
    #44064
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    2xStrep tag, 6xGly linker, ADH1 transcription ter…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44064-plasmid-map-sequence-id-61680

    Enlarge

  8. pUASp YFP Rholike DN

    Plasmid
    #52240
    Purpose
    Drosophila expression of YFP-Rholike dominant negative mutant
    Depositor
    Matthew Scott
    Insert
    Rholike (RhoL Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    T25N; DN mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. pAc.1B-EGFP-DmHrb98

    Plasmid
    #66595
    Purpose
    Expresses EGFP-tagged Hrb98 in Drosophila cells
    Depositor
    Elisa Izaurralde
    Insert
    Hrb98 (Hrb98DE Fly)
    Use
    Drosophila expression
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    Actin 5C
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    66595-plasmid-map-sequence-id-113871

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  10. pCRII-Topo Lhx2

    Plasmid
    #45873
    Depositor
    Jeffrey Macklis
    Insert
    Lhx2 in situ probe (Lhx2 Mouse)
    Use
    In situ
    Tags
    Expression
    Mutation
    contains bp# 1809-2217 of NM_010710.3
    Promoter
    Available since
    July 5, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. pJK693

    Plasmid
    #72382
    Purpose
    Produces Escherichia coli cysteine desulfurase IscS with Ala260>Thr mutation, N-terminal His6-tag (H6EcIscS-A260T)
    Depositor
    T. J. Kappock
    Insert
    cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent (iscS Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated alanine-260 to threonine
    Promoter
    T7
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72382-plasmid-map-sequence-id-134008

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  12. pLV-Enh eGFP Reporter-muEDC-Int3

    Plasmid
    #59288
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intronic region of the mouse Epidermal Differentiation Complex.
    Depositor
    Benjamin Yu
    Insert
    mFlg int-Intron 1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pPyCAG-Nanog(Y136A)-IP

    Plasmid
    #62995
    Purpose
    Expresses Mouse Nanog Y136A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Y136A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Tyrosine 136 (137 AA in Mouse Protein) to…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62995-plasmid-map-sequence-id-104597

    Enlarge

  14. p14-9VNA5

    Plasmid
    #50931
    Purpose
    Expresses human SRP14A5 and SRP9 proteins as a fusion protein in mammalian cells.
    Depositor
    Katharina Strub
    Insert
    SRP14 and SRP9 (SRP14, SRP9 Human)
    Use
    Tags
    VN tag and flag tag
    Expression
    Mammalian
    Mutation
    Amino acids 96-100 of SRP14 changed to alanine
    Promoter
    CMV
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pZC05 (pFA6a-TEV-6xGly-PrtA-KanMX)

    Plasmid
    #44096
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Expression
    Mutation
    Promoter
    Available since
    April 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    44096-plasmid-map-sequence-id-61688

    Enlarge

  16. CSNK1G2

    Plasmid
    #39150
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CSNK1G2 (CSNK1G2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. pIRES-Neo-HA-cdc25C1

    Plasmid
    #40966
    Depositor
    Olivier Gavet
    Insert
    cdc25C5 (CDC25C Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pZC06 (pFA6a-PSc-6xGly-PrtA-KanMX)

    Plasmid
    #44090
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Expression
    Mutation
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44090-plasmid-map-sequence-id-61689

    Enlarge

  20. Lmx1a-in situ probe

    Plasmid
    #45071
    Depositor
    Kathleen Millen
    Insert
    Lmx1a LIM homeobox transcription factor 1 alpha (residues 94-1149) (Lmx1a Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Lmx1a residues 94-1149
    Promoter
    Available since
    May 30, 2013
    Availability
    Academic Institutions and Nonprofits only

  21. pCVL RipTAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers

    Plasmid
    #50626
    Purpose
    pRipTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50626-plasmid-map-sequence-id-79595

    Enlarge

  22. SLC9A3R2

    Plasmid
    #38948
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SLC9A3R2 (NHERF2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  25. pZC07 (pFA6a-TEV-6xGly-PrtA-HphMX)

    Plasmid
    #44084
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Expression
    Mutation
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44084-plasmid-map-sequence-id-61690

    Enlarge

  26. pUASP Flag Rab32 WT

    Plasmid
    #44833
    Depositor
    Matthew Scott
    Insert
    Rab32 (Rab32 Fly)
    Use
    Drosophila
    Tags
    Flag
    Expression
    Insect
    Mutation
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  27. ENOSF1

    Plasmid
    #39104
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ENOSF1 (ENOSF1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  28. pIRES-Neo-HA-cdc25C5

    Plasmid
    #40967
    Depositor
    Olivier Gavet
    Insert
    cdc25C5 (CDC25C Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  29. BC-GPRC

    Plasmid
    #44199
    Depositor
    Lei Xu
    Insert
    human GPR56 C-terminal fragment (ADGRG1 Human)
    Use
    Retroviral
    Tags
    none
    Expression
    Mammalian
    Mutation
    C-terminal fragment of GPR56 starting at amino a…
    Promoter
    LTR
    Available since
    May 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    44199-plasmid-map-sequence-id-60058

    Enlarge

  30. pTF260 (pFA6a-PSc-6xGly-KanMX)

    Plasmid
    #44086
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44086-plasmid-map-sequence-id-61663

    Enlarge

  31. pJK142

    Plasmid
    #73317
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) as a fusion to Pas2p, with c-myc tag and His6-tag
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Use
    Tags
    His6, Pas2p, and c-myc
    Expression
    Yeast
    Mutation
    synthetic gene. Encodes a glycine-204 to serine m…
    Promoter
    AOX1
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73317-plasmid-map-sequence-id-140432

    Enlarge

  32. pUC57-sgDedd-1

    Plasmid
    #74435
    Purpose
    sgRNA expression vector for mouse Dedd gene targeting intron 5
    Depositor
    Petra Arck
    Insert
    Dedd (Dedd Mouse)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  33. pPyCAG-Nanog(K137A)-IP

    Plasmid
    #62996
    Purpose
    Expresses Mouse Nanog K137A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog K137A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Lysine 137 (138 AA in Mouse Protein) to A…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62996-plasmid-map-sequence-id-104598

    Enlarge

  34. pPyCAG-Nanog(Y119A)-IP

    Plasmid
    #62991
    Purpose
    Expresses Mouse Nanog Y119A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Y119A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Tyrosine 119 (120 AA in Mouse Protein) to…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62991-plasmid-map-sequence-id-104440

    Enlarge

  35. pIRES-GFP-hFJX1-myc

    Plasmid
    #46928
    Purpose
    coexpression of FJX1-myc and GFP in mammalian cells
    Depositor
    Natasha Deane, R.D. Beauchamp
    Insert
    FJX1 (FJX1 Human)
    Use
    Tags
    myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 13, 2013
    Availability
    Academic Institutions and Nonprofits only

  36. ACBD4

    Plasmid
    #39009
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACBD4 (ACBD4 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  38. pUASp YFP Rholike CA

    Plasmid
    #52241
    Purpose
    Drosophila expression of YFP-Rholike constitutively active mutant
    Depositor
    Matthew Scott
    Insert
    Rholike (RhoL Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Q69L; CA mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  39. 4LG9

    Plasmid
    #60096
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TBL1XR1:APC048-A06:C27585 (TBL1XR1 TBL1XR1:APC048-A06:C27585, Human)
    Use
    Baculovirus expression
    Tags
    6xHis
    Expression
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  40. pTF265 (pFA6a-PSc-6xGly-12xHis-His3MX)

    Plasmid
    #44063
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    12xHis tag, 6xGly linker, ADH1 transcription term…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44063-plasmid-map-sequence-id-61668

    Enlarge

Showing: 4801 - 4840 of 8387 results