Skip to main content
Addgene
Showing: 2401 - 2420 of 2760 results

  2. pGBW-m4046785

    Plasmid
    #145684
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 N (nucleocapsid)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145684-plasmid-map-sequence-id-297841

    Enlarge

  3. pRelA'

    Plasmid
    #175595
    Purpose
    Tet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Catalytic domain of E.coli relA gene.
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Only the catalytic domain of the enzyme is used (…
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175595-plasmid-map-sequence-id-348284

    Enlarge

  4. pMesh1

    Plasmid
    #175594
    Purpose
    Lac inducible, AMP Resistant, non labelled gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Mesh1 (Mesh1 Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized for translation in E.coli. AGGAGG…
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175594-plasmid-map-sequence-id-348364

    Enlarge

  6. His-NavMs

    Plasmid
    #100004
    Purpose
    Electrophysiology in mammalian cells: Mammalian expression of voltage gated sodium channel and GFP
    Depositor
    Paul DeCaen
    Article
    Sula et al Nat Commun. 2017 Feb 16;8:14205. doi: 10
    Insert
    His-NavMs, Voltage gated sodium channel
    Use
    Tags
    Hexa-His tag followed by a thrombin cleavage site
    Expression
    Mammalian
    Mutation
    C-terminus from a codon-optimized synthetic gene,…
    Promoter
    CMV
    Available since
    Aug. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100004-plasmid-map-sequence-id-192452

    Enlarge

  7. pGL3 Pro E2

    Plasmid
    #48749
    Purpose
    Expression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)
    Depositor
    Joseph Takahashi
    Article
    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10
    Insert
    mPer2 promoter/enhancer region containing E-box2 (-112 to +98)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    48749-plasmid-map-sequence-id-448241

    Enlarge

  8. AA011

    Plasmid
    #215973
    Purpose
    Fragmid fragment: (N' terminus) for C>T base editing
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    Start; BPNLS_v1.1; rAPOBEC1_v1.1; Linker_v1.1
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215973-plasmid-map-sequence-id-431523

    Enlarge

  9. AA013

    Plasmid
    #215975
    Purpose
    Fragmid fragment: (N' terminus) for A>G base editing
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    Start; BPNLS_v1.1; ABE8e_v1.1; Linker_v1.1
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215975-plasmid-map-sequence-id-431394

    Enlarge

  10. pL0-MtPT4Pro

    Plasmid
    #159414
    Purpose
    Medicago truncatula PT4 promoter sequence (836 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.
    Depositor
    Sam Brockington
    Article
    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d
    Insert
    MtPT4 Promoter
    Use
    Puc19-derived
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    159414-plasmid-map-sequence-id-323169

    Enlarge

  11. pL0-MtBCP1Pro

    Plasmid
    #159415
    Purpose
    Medicago truncatula BCP1 promoter sequence (1108 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.
    Depositor
    Sam Brockington
    Article
    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d
    Insert
    MtBCP1 Promoter
    Use
    Puc19-derived
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    159415-plasmid-map-sequence-id-323165

    Enlarge

  16. pGBW-m4046757

    Plasmid
    #145657
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145657-plasmid-map-sequence-id-298331

    Enlarge

  17. pET151-D-TOPO-PARP14-SΔK

    Plasmid
    #216658
    Purpose
    Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.
    Depositor
    Herwig Schüler
    Article
    Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.
    Insert
    PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Human, Synthetic)
    Use
    Tags
    6xHis-V5-TEV
    Expression
    Bacterial
    Mutation
    Deletion starting from Q1390 to D1448 and substit…
    Promoter
    T7
    Available since
    Aug. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    216658-plasmid-map-sequence-id-429340

    Enlarge

Showing: 2401 - 2420 of 2760 results