We narrowed to 5,473 results for: chia

1. pBN082

   Plasmid

   #173444

   Purpose

   express E. coli E69 (O9a:K30) Wzc_K540M with Y706E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc_K540M with Y706E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M, Y706E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBN083

   Plasmid

   #173445

   Purpose

   express E. coli E69 (O9a:K30) Wzc_K540M with Y705E, Y706E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc_K540M with Y705E, Y706E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M, Y705E, Y706E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBN084

   Plasmid

   #173446

   Purpose

   express E. coli E69 (O9a:K30) Wzc_K540M with Y708E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc_K540M with Y708E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M, Y708E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBN085

   Plasmid

   #173447

   Purpose

   express E. coli E69 (O9a:K30) Wzc_K540M with Y713E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc_K540M with Y713E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M, Y713E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBN008

   Plasmid

   #167949

   Purpose

   express E. coli E69 (O9a:K30) Wzc with K540M mutation

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30), K540M nutant
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBN093

   Plasmid

   #167951

   Purpose

   express E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M, delete motif 3

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBN074

   Plasmid

   #167955

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y705E, Y706E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y705E, Y706E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y705E, Y706E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTJV1ScGm-rpoB

   Plasmid

   #166979

   Purpose

   Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aacC1 for gentamycin resistance

   Depositor

   Christopher Reisch

   Article

   Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

   Insert

   gentamycin-3-acetyltransferase (aac(3)-Ia )
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EcNusGKOW

   Plasmid

   #113124

   Purpose

   Expresses C-terminus of E. coli NusG

   Depositor

   James Berger

   Article

   Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

   Insert

   NusG (nusG Escherichia coli)
   

   Use

   Tags

   His6-MBP-N10-TEV

   Expression

   Bacterial

   Mutation

   Deleted residues 1-124

   Promoter

   T7

   Available since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EcRfaHKOW

    Plasmid

    #113125

    Purpose

    Expresses C-terminus of E. coli RfaH

    Depositor

    James Berger

    Article

    Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

    Insert

    RfaH (rfaH Escherichia coli)
    

    Use

    Tags

    His6-MBP-N10-TEV

    Expression

    Bacterial

    Mutation

    Deleted residues 1-107

    Promoter

    T7

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pME-QUASM1:GFPNLS-SV40pA

    Plasmid

    #155116

    Purpose

    Middle entry vector containing QUASM1 element upstream of GFP-NLS.

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QUASM1:GFPNLS
    

    Use

    Gateway middle entry vector

    Tags

    Expression

    Mutation

    Promoter

    QUASM1-E1b

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIIS18-BufAI-2

    Plasmid

    #128651

    Purpose

    Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

    Depositor

    Tohru Suzuki

    Article

    Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIIS18-BufAI-1

    Plasmid

    #128650

    Purpose

    Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

    Depositor

    Tohru Suzuki

    Article

    Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPB-DEST-R5R4-pNeo

    Plasmid

    #141058

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Neomycin resistance gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGBW-m4136457

    Plasmid

    #149537

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGBW-m4095418

    Plasmid

    #149525

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    d1-1088 d1203-1945

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pGBW-m4046784

    Plasmid

    #145704

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF6 protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF6 protein (ORF6 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pGBW-m4046787

    Plasmid

    #145690

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp9

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGBW-m4046767

    Plasmid

    #145692

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF6 protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF6 protein (ORF6 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pGBW-m4046727

    Plasmid

    #145686

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF3a protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge