We narrowed to 18,091 results for: multi

1. pCAM-kan-R4R3

   Plasmid

   #71275

   Purpose

   Binary destination vector for plant transformation. Kanamycin resistance.

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Type

   Empty backbone

   Use

   Multisite gateway

   Available Since

   Dec. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-MCS

   Plasmid

   #75174

   Purpose

   Multiple cloning site for insertion of gene of interest for C-terminal fusions.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   Multiple Cloning Site
   

   Use

   Three prime entry vector for multisite gateway th…

   Promoter

   n/a

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP

   Plasmid

   #66707

   Purpose

   expresses a single gRNA and VP64-dCas9-VP64

   Depositor

   Charles Gersbach

   Inserts

   hU6-gRNA expression cassette
   S.Pyogenes VP64-dCas9-VP64
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   VP64

   Mutation

   D10A and H840A

   Promoter

   hU6 and hUbC

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-flag-GFP-Intb1

   Plasmid

   #205193

   Purpose

   This vector encodes of a synCAM (Intb1) and GFP

   Depositor

   Wendell Lim

   Article

   Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

   Insert

   synCAM Intb1 and GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pScI_dCas9-CDA-UL

   Plasmid

   #108551

   Purpose

   Bacterial Target-AID UGI-LVA vector

   Depositor

   Akihiko Kondo

   Article

   Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

   Insert

   dCas9-PmCDA1-UGI-LVA
   

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   D10A and H840A for SpCas9

   Promoter

   lambda OR

   Available Since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_miniSOG2 T2A H2B-EGFP

   Plasmid

   #87410

   Purpose

   Mammalian expression vector encoding Histone 2B GFP fusion and miniSOG2

   Depositor

   Xiaokun Shu

   Article

   Makhijani et al Cell Chem Biol. 2017 Jan 19;24(1):110-11

   Insert

   mini singlet oxygen generator 2
   

   Expression

   Mammalian

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-flag-GFP-MUC4

   Plasmid

   #205189

   Purpose

   This vector encodes of a synCAM (MUC4) and GFP

   Depositor

   Wendell Lim

   Article

   Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

   Insert

   synCAM MUC4 and GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pspCas9-HF-tetra-com-vector

   Plasmid

   #132555

   Purpose

   Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.

   Depositor

   Baisong Lu

   Article

   Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

   Inserts

   High fidelity Cas9
   sgRNA scaffold with com replacing the Tetraloop
   

   Expression

   Mammalian

   Available Since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-fDIO-mCherryP2ACre

   Plasmid

   #161773

   Purpose

   Flp-Dependent mCherry and Cre expression

   Depositor

   Baoji Xu

   Article

   An et al Nat Commun. 2020 Apr 7;11(1):1729. doi:

   Insert

   mCherry-P2A-Cre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRG713_lexO-HO_LexA-TF_URA3MX

    Plasmid

    #154821

    Purpose

    Inducible expression of HO endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    HO endonuclease (HO Budding Yeast)
    LexA-ER-B112
    

    Tags

    FLAG

    Expression

    Yeast

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. 2xMyc-tdMCP-SNAP (tdMCP standard)

    Plasmid

    #226291

    Purpose

    Lentiviral plasmid expressing a tdMCP-protein standard. Used in LABEL-seq abundance assays.

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Inserts

    2xMyc-tdMCP-SNAPtag
    EGFP-IRES-BlastR
    

    Use

    Lentiviral

    Tags

    2xMyc-tdMCP

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRG705_lexO-AsiSI_LexA-TF_LEU2MX

    Plasmid

    #154817

    Purpose

    Inducible expression of AsiSI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    AsiSI endonuclease
    LexA-ER-B112
    

    Tags

    FLAG and NLS

    Expression

    Yeast

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hsyn-FLPo-T2A-GFP

    Plasmid

    #161766

    Purpose

    express FLPo and GFP under the control of human synapsin promoter

    Depositor

    Baoji Xu

    Article

    An et al Nat Commun. 2020 Apr 7;11(1):1729. doi:

    Inserts

    FLPo
    T2AEGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSynapsin1

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-CMVSP6-nEGFP-SV40-PURO

    Plasmid

    #138364

    Purpose

    Lentiviral plasmid for nuclear EGFP expression driven by CMVSP6 and puromycin resistance under SV40 promoter for selection

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    nuclear EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. MF87_PB-EF1a-TET3G-P2A-ERT2-Gal4-BGHpA

    Plasmid

    #176630

    Purpose

    Integrated in CHO-K1 to make parental polyclonal lines for all other MultiFate cell lines

    Depositor

    Michael Elowitz

    Article

    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

    Insert

    EF1a-TET3G-P2A-ERT2-Gal4-BGHpA
    

    Use

    Synthetic Biology

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGE1109

    Plasmid

    #202773

    Purpose

    CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:TREX2-zCas9_ttriple
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDAI-SceI-SacB

    Plasmid

    #113635

    Purpose

    Broad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacB

    Depositor

    Miguel Valvano

    Article

    Aubert et al Methods Mol Biol. 2014;1197:311-27. doi:

    Insert

    sacB
    

    Expression

    Bacterial

    Mutation

    Please see Depositor Comments

    Promoter

    sacB promoter

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCCl-MCS_miniCMV_miRFP703

    Plasmid

    #134985

    Purpose

    The plasmid includes a miRFP703 gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

    Depositor

    Claudio Mussolino

    Article

    Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

    Insert

    miRFP703
    

    Use

    Lentiviral

    Promoter

    minCMV

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. DHFR-PP7-VP64_T2A_GFP

    Plasmid

    #86167

    Purpose

    Expresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

    Insert

    DHFR-PP7-VP64_T2A_GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Destabilized domain (N-terminus version) of E.col…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRG706_lexO-SbfI_LexA-TF_LEU2MX

    Plasmid

    #154818

    Purpose

    Inducible expression of SbfI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    SbfI endonuclease
    LexA-ER-B112
    

    Tags

    FLAG and NLS

    Expression

    Yeast

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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