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6xHis-SNAP-PI(4)P Probe

Plasmid

#211509

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteria

Depositor

David Murray, Hannes Maib

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

SidC (P4C)

Use

Tags

6xhis-SNAP

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(4,5)P2 Probe

Plasmid

#211513

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteria

Depositor

David Murray, Hannes Maib

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

PLCdelta1-PH

Use

Tags

6xhis-SNAP

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRoquin1_AE

Plasmid

#148611

Purpose

Mammalian Expression of HsRoquin1

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsRoquin1 (RC3H1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pNP1954

Plasmid

#222730

Purpose

pNP1723 - IS621 WT Donor and bridgeRNA in trans (TBL-T5, DBL-WT)

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 bridge RNA

Use

Tags

sfGFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pNP1723

Plasmid

#222727

Purpose

sfGFP-ON BsaI Golden Gate (GG) Landing Pad for LD-RD and J23119-BsaI GG Landing Pad for bRNA (bRNA in trans expression)

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Type

Empty backbone

Use

Tags

sfGFP

Expression

Bacterial

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsXRN1_K

Plasmid

#146784

Purpose

Mammalian Expression of HsXRN1

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

HsXRN1 (XRN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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hSyn-IRES2-GFP

Plasmid

#234875

Purpose

Lentiviral plasmid with a multiple cloning site followed by IRES2-GFP.

Depositor

Andrew Arrant

Article

Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hSYn

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-92a

Plasmid

#22767

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro RNA 92a (mir-92a primary transcript, Fly)

Use

Drosophila expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI Probe

Plasmid

#211507

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol (PI) from bacteria

Depositor

David Murray, Hannes Maib

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

BcPI-PLC-ANH

Use

Tags

6xhis-SNAP

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-mArg1 promoter/enhancer -31/-3810

Plasmid

#34571

Depositor

Peter Murray

Article

Pauleau et al J Immunol. 2004 Jun 15;172(12):7565-73.

Insert

Arg1 promoter/enhancer -31/-3810 (Arg1 Mouse)

Use

Luciferase

Tags

Firefly Luciferase

Expression

Mutation

Promoter

Available since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pET-MCN-10His-mStayGold (E138D)

Plasmid

#211361

Purpose

Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Article

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

mStayGold

Use

Tags

10xHis-tag

Expression

Bacterial

Mutation

E138D

Promoter

T7

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hNox4

Plasmid

#69352

Purpose

expresses human Nox4 in mammalian cells

Depositor

Karl-Heinz Krause

Article

Serrander et al Biochem J. 2007 Aug 15;406(1):105-14.

Insert

hNOX4 (NOX4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-mArg1 base promoter -31/-2365

Plasmid

#34570

Depositor

Peter Murray

Article

Pauleau et al J Immunol. 2004 Jun 15;172(12):7565-73.

Insert

Arg1 base promoter -31/-2365 (Arg1 Mouse)

Use

Luciferase

Tags

Firefly Luciferase

Expression

Mutation

Promoter

Available since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only
pMA-SpCas9-g3

Plasmid

#80786

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 3/13/23 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg5_T

Plasmid

#147552

Purpose

Mammalian Expression of HsSmg5

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5 (SMG5 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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PT7-deGFP-MGapt

Plasmid

#67741

Purpose

T7 promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T7term

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PT7

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsAGO1_F

Plasmid

#146314

Purpose

Mammalian Expression of HsAGO1

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO1 (AGO1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Zeo-CnB

Plasmid

#191965

Purpose

Expression of CnB (calcineurin subunit beta).

Depositor

Luca Scorrano

Article

Cereghetti et al Proc Natl Acad Sci U S A. 2008 Oct 14;10

Insert

Calcineurin subunit beta (PPP3CB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCE048-SiT-Cas12a

Plasmid

#128124

Purpose

Express both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET-MCN-10His-StayGold

Plasmid

#211360

Purpose

Bacterial expression of the bright and photostable StayGold fluorescent protein. Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Article

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

StayGold

Use

Tags

10xHis-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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