We narrowed to 12,292 results for: BASE

1. pCMV-T7-ABE8.20m-SpCas9-NRCH-P2A-EGFP (RMD55)

   Plasmid

   #197501

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRCH-BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8.20 mutations in TadA and nSpCas9-NRCH(D10A/I…

   Promoter

   CMV and T7

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FRE5

   Plasmid

   #62377

   Purpose

   Fluorescent reporter to quantify the ratio of two alternative splicing patterns.

   Depositor

   Thomas Cooper

   Article

   Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

   Insert

   dsRed and GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-BE4max-P2A-EGFP (pRZ33)

   Plasmid

   #140083

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP

   Depositor

   Keith Joung

   Insert

   Human codon optimized pCAG BE4max with P2A-EGFP
   

   Use

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   D10A (SpCas9)

   Promoter

   CAG

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p667-UBC-GFP-V5_IDG-K

   Plasmid

   #135242

   Purpose

   Gateway destination clone of GFP (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   GFP-V5
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Jan. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3 H2B-mIFP T2A Mpro (C145A)

   Plasmid

   #163080

   Purpose

   Expresses inactive Mpro mutant and a near-infrared fluorescent protein mIFP fused to H2B

   Depositor

   Xiaokun Shu

   Insert

   Inactive Mpro mutant C145A and H2B-mIFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRHA12

   Plasmid

   #138942

   Purpose

   AND gate with IPTG and aTc as inputs and sfGFP as output

   Depositor

   Guillermo Rodrigo

   Article

   Rosado et al PLoS Genet. 2018 Jul 19;14(7):e1007548.

   Insert

   riboregulated sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSG10-pGlnA-mCherry

   Plasmid

   #102467

   Purpose

   Expresses mCherry fluorescent protein in E. coli. The expression is controlled by a pGlnA promoter.

   Depositor

   Richard Murray

   Article

   Guo et al bioRxiv 122606

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pGlnA

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p663-UBC-V5-dsRed_IDG-K

   Plasmid

   #135262

   Purpose

   Gateway destination clone of dsRed (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   V5-dsRed
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDGB3_alpha1_pNOS-Rep-RepA-Tnos (GB3598)

   Plasmid

   #225432

   Purpose

   Transcriptional unit of BeYDV Rep/RepA (C1:C2)

   Depositor

   Diego Orzaez

   Insert

   pNOS:Rep/RepA:tNOS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRHA40

    Plasmid

    #138941

    Purpose

    XOR gate with IPTG and aTc as inputs and mRFP1 as output

    Depositor

    Guillermo Rodrigo

    Article

    Rosado et al PLoS Genet. 2018 Jul 19;14(7):e1007548.

    Insert

    riboregulated mRFP1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge