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Showing: 2421 - 2440 of 13796 results

  1. LSB-hsa-miR-19b-1-5p

    Plasmid
    #103325
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-19b-1-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-19b-1-5p target (MIR19B1 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103325-plasmid-map-sequence-id-195699

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  3. pGEX-6P-3-F37A+L196F+V252A+C253G-hADPRM

    Plasmid
    #107163
    Purpose
    Expresses specific cyclic-ADP-ribose (cADPR) phosphohydrolase in Escherichia coli as a GST fusion protein that can be cut with PreScission(TM) protease to yield active enzyme
    Depositor
    Jose Carlos Cameselle
    Article
    Ribeiro et al Sci Rep. 2018 Jan 18;8(1):1036. doi: 10.
    Insert
    F37A+L196F+V252A+C253G-hADPRM GST-mutant cADPR phosphohydrolase gene
    Tags
    GST
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107163-plasmid-map-sequence-id-204806

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  4. pNOC_dfnCas12a_crRNA NC

    Plasmid
    #176256
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and spacer sequence is replaced by the type IIS restriction site for endonuclease BaeI that can be
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176256-plasmid-map-sequence-id-346577

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  5. LSB-hsa-miR-522-3p

    Plasmid
    #103614
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-522-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-522-3p target (MIR522 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103614-plasmid-map-sequence-id-196276

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  6. LSB-hsa-miR-183-5p

    Plasmid
    #103285
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-183-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-183-5p target (MIR183 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103285-plasmid-map-sequence-id-195619

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  7. LSB-hsa-miR-483-5p

    Plasmid
    #103551
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-483-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-483-5p target (MIR483 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103551-plasmid-map-sequence-id-196150

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  8. LSB-hsa-miR-20a-3p

    Plasmid
    #103341
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-20a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-20a-3p target (MIR20A Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103341-plasmid-map-sequence-id-195731

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  9. LSB-hsa-miR-193a-3p

    Plasmid
    #103305
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-193a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-193a-3p target (MIR193A Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103305-plasmid-map-sequence-id-195659

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  10. pAAV-CMV-dSa VP64 Actc1

    Plasmid
    #99691
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Actc1 promoter, vector allows for activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG) (Actc1 Mouse, Synthetic)
    Use
    AAV and CRISPR
    Tags
    VP64
    Mutation
    dead Cas9
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    99691-plasmid-map-sequence-id-194530

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  13. KB501: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; CMV promoter

    Plasmid
    #121813
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    March 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    121813-plasmid-map-sequence-id-239026

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  14. pAAV-CMV-dSa VP64 Neurog2

    Plasmid
    #99695
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Neurog2 promoter, vector allows for activation of mouse Neurog2, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Neurog2 (gRNA: GGTATATAAGGGGTTTTAAG) (Neurog2 Mouse, Synthetic)
    Use
    AAV and CRISPR
    Tags
    VP64
    Mutation
    dead Cas9
    Available Since
    Sept. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    99695-plasmid-map-sequence-id-194570

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  15. LSB-hsa-miR-302a-3p

    Plasmid
    #103400
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-302a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-302a-3p target (MIR302A Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103400-plasmid-map-sequence-id-195849

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  16. LSB-hsa-miR-885-3p

    Plasmid
    #103744
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-885-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-885-3p target (MIR885 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103744-plasmid-map-sequence-id-196536

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  17. LSB-hsa-miR-579-5p

    Plasmid
    #103661
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-579-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-579-5p target (MIR579 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103661-plasmid-map-sequence-id-196370

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  18. LSB-hsa-miR-510-5p

    Plasmid
    #103593
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-510-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-510-5p target (MIR510 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103593-plasmid-map-sequence-id-196234

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  19. LSB-hsa-miR-483-3p

    Plasmid
    #103550
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-483-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-483-3p target (MIR483 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103550-plasmid-map-sequence-id-196148

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  20. LSB-hsa-miR-495-3p

    Plasmid
    #103568
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-495-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-495-3p target (MIR495 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103568-plasmid-map-sequence-id-196184

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Showing: 2421 - 2440 of 13796 results