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pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-PuroR-SMAR-g5

Plasmid

#208377

Purpose

Episomal plasmid for expression of 2 proteins with puromycin selection and EGFP monitoring

Depositor

Daniel Hawiger

Article

Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

Type

Empty backbone

Expression

Mammalian

Promoter

human CMV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCBFD1/HBFD1Bad

Plasmid

#53364

Purpose

Will convert beta-carotene into astaxanthin in E. coli when expression of the hbfd1 cDNA is induced with arabinose. Use with pAC-BETA or pAC-BETAipi.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

cbfd1

Expression

Bacterial

Promoter

lac

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGreen_GUS_competitor

Plasmid

#55208

Purpose

GUS-based microRNA target competitor used for testing function as target mimicry

Depositor

Michael Axtell

Article

Liu et al Plant Cell. 2014 Feb;26(2):741-53. doi:

Insert

beta-glucuronidase

Use

Plant expression

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pZA69

Plasmid

#174782

Purpose

RNAi

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

Intron-containing hairpin RNA (ihpRNA)-NbZAR1

Use

RNAi

Mutation

WT

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-protein1-IRES-protein2-IRESatt-cTermIntein-cTermPuroR

Plasmid

#208379

Purpose

Episomal plasmid - for expression of 2 proteins linked with c-terminal split intein and split puromycin resistance

Depositor

Daniel Hawiger

Article

Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

Type

Empty backbone

Expression

Mammalian

Promoter

human CMV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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AL13Rb

Plasmid

#161006

Purpose

PP7-tagged reporter without a promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Type

Empty backbone

Tags

PP7 and H2B-mScarlet

Expression

Plant

Available Since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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AL12R

Plasmid

#161007

Purpose

PP7-tagged reporter without a promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Type

Empty backbone

Tags

PP7 and H2B-mScarlet

Expression

Plant

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pLSLT

Plasmid

#51498

Purpose

pLSL with T30 TALEN followed by us:NPTII repair template, Rep is not included on this plasmid

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

T30 TALEN pair
us:NPTII repair template

Use

Plant t-dna plasmid

Promoter

virion-sense LIR and 2x35S

Available Since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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pMB109

Plasmid

#223017

Purpose

Cas9 and Csy4 with 5' and 3' targets (II) for Lotus callus assay

Depositor

Martin Parniske

Article

Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

Insert

Cas9 and Csy4 with 5' and 3' targets (II)

Use

CRISPR

Expression

Plant

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMB20

Plasmid

#223004

Purpose

Csy4 with 5' and 3' targets (I) and without Cas for Lotus callus assay

Depositor

Martin Parniske

Article

Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

Insert

Csy4 with 5' and 3' targets (I)

Use

CRISPR

Expression

Plant

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMB21

Plasmid

#223005

Purpose

Csy4 with 5' and 3' targets (II) and without Cas for Lotus callus assay

Depositor

Martin Parniske

Article

Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

Insert

Csy4 with 5' and 3' targets (II)

Use

CRISPR

Expression

Plant

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMB23

Plasmid

#223007

Purpose

Csy4 with 5' and 3' targets (I) and without Cas for Nicotiana leaf assay

Depositor

Martin Parniske

Article

Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

Insert

Csy4 with 5' and 3' targets (I)

Use

CRISPR

Expression

Plant

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMB26

Plasmid

#223009

Purpose

Cas12a and Csy4 with 5' target (I) for Nicotiana leaf assay

Depositor

Martin Parniske

Article

Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

Insert

Cas12a and Csy4 with 5' target (I)

Use

CRISPR

Expression

Plant

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMB27

Plasmid

#223010

Purpose

Cas12a and Csy4 with 3' target (I) for Nicotiana leaf assay

Depositor

Martin Parniske

Article

Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

Insert

Cas12a and Csy4 with 3' target (I)

Use

CRISPR

Expression

Plant

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMB28

Plasmid

#223011

Purpose

Cas12a and Csy4 with 5' and 3' targets (I) for Nicotiana leaf assay

Depositor

Martin Parniske

Article

Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

Insert

Cas12a and Csy4 with 5' and 3' targets (I)

Use

CRISPR

Expression

Plant

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB45

Plasmid

#223012

Purpose

Cas12a and Csy4 with 5' and 3' targets (I) for Lotus callus assay

Depositor

Martin Parniske

Article

Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

Insert

Cas12a and Csy4 with 5' and 3' targets (I)

Use

CRISPR

Expression

Plant

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMB51

Plasmid

#223013

Purpose

Cas12a and Csy4 with 5' and 3' targets (II) for Lotus callus assay

Depositor

Martin Parniske

Article

Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

Insert

Cas12a and Csy4 with 5' and 3' targets (II)

Use

CRISPR

Expression

Plant

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMB61

Plasmid

#223014

Purpose

Cas12a and Csy4 with 5' and 3' targets (I) for Nicotiana leaf assay

Depositor

Martin Parniske

Article

Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

Insert

Cas12a and Csy4 with 5' and 3' targets (I)

Use

CRISPR

Expression

Plant

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB105

Plasmid

#223016

Purpose

Cas9 and Csy4 with 5' and 3' targets (I) for Nicotiana leaf assay

Depositor

Martin Parniske

Article

Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

Insert

Cas9 and Csy4 with 5' and 3' targets (I)

Use

CRISPR

Expression

Plant

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMpGWBhis03-Citrine

Plasmid

#188557

Purpose

Binary vector for the expression of Citrine with MpIGPDm selective marker in Marchantia polymorpha (igpd mutants) .

Depositor

Yutaka Kodama

Article

Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

Insert

Citrine and MpIGPDm

Use

CRISPR and Gateway Cloning

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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