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We narrowed to 2,467 results for: PUC19

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Showing: 2421 - 2440 of 2467 results

  1. pYI2294

    Plasmid
    #228352
    Purpose
    Methanol-inducible ALX-0081 expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
    Use
    Affinity Reagent/ Antibody
    Tags
    AAA linker followed by FLAG-His6 and MF_ secretio…
    Expression
    Yeast
    Promoter
    KpAOX1 promoter
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228352-plasmid-map-sequence-id-457091

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  2. pYI2293

    Plasmid
    #228351
    Purpose
    Constitutive ALX-0081 expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
    Use
    Affinity Reagent/ Antibody
    Tags
    AAA linker followed by FLAG-His6 and MF_ secretio…
    Expression
    Yeast
    Promoter
    KpGAPDH promoter
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228351-plasmid-map-sequence-id-457078

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  3. qTAG-N-Blast-moxGFP-LMNB1

    Plasmid
    #207772
    Purpose
    Donor template for Blast-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Blast-moxGFP Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Feb. 14, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    207772-plasmid-map-sequence-id-415638

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  4. qTAG-N-Blast-miniTurbo-LMNB1

    Plasmid
    #207774
    Purpose
    Donor template for Blast-2A-miniTurbo insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Blast-miniTurbo Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207774-plasmid-map-sequence-id-416197

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  6. pVEZF1-donor

    Plasmid
    #112341
    Purpose
    CRISPR donor plasmid to tag human transcription factor VEZF1 with GFP
    Depositor
    Kevin White
    Insert
    VEZF1 homology arms flanking EGFP-IRES-Neo cassette (VEZF1 Human)
    Use
    CRISPR
    Mutation
    rs999907002, hg38:17:57975308:A>T, hg38:17:579…
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112341-plasmid-map-sequence-id-229654

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  7. qTAG-C-mStayGold-EFS-Puro-PLK4

    Plasmid
    #227311
    Purpose
    Donor template for mStayGold-EFS-Puro insertion into the C-terminus of the PLK4 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    PLK4 Homology Arms flanking a mStayGold-EFS-Puro Cassette (PLK4 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227311-plasmid-map-sequence-id-452079

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  8. qTAG-C-Solo-mScarlet-H3C2

    Plasmid
    #207781
    Purpose
    Donor template for mScarlet insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H3C2 Homology Arms flanking a mScarlet Tag (H3C2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207781-plasmid-map-sequence-id-416187

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  12. pNOC_dfnCas12a-KRAB_crRNA Td2

    Plasmid
    #176261
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176261-plasmid-map-sequence-id-346586

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  13. pYI2216

    Plasmid
    #228349
    Purpose
    2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0081 expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
    Use
    Affinity Reagent/ Antibody
    Tags
    AAA linker followed by FLAG-His6 and MF_ secretio…
    Expression
    Yeast
    Promoter
    2,4-Diacetylphloroglucinol-regulatable synthetic …
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228349-plasmid-map-sequence-id-457093

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  14. pMT1461

    Plasmid
    #228319
    Purpose
    Doxycycline-regulatable ALX-0081 expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-ALX-0081-3A-FLAG-His
    Use
    Affinity Reagent/ Antibody
    Tags
    AAA linker followed by FLAG-His6 and MF_ secretio…
    Expression
    Yeast
    Promoter
    Doxycycline-regulatable synthetic promoter (KpTc7…
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228319-plasmid-map-sequence-id-457683

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  15. qTAG-C-Solo-mStayGold-H3C2

    Plasmid
    #227334
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H3C2 Homology Arms flanking a mStayGold Tag (H3C2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    227334-plasmid-map-sequence-id-453638

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  16. qTAG-C-Solo-miRFP670nano3-H3C2

    Plasmid
    #227335
    Purpose
    Donor template for miRFP670nano3 insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H3C2 Homology Arms flanking a miRFP670nano3 Tag (H3C2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227335-plasmid-map-sequence-id-452142

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  18. B2M-SDMutation-EhT

    Plasmid
    #215546
    Purpose
    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
    Depositor
    Kai Wang
    Article
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Insert
    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)
    Use
    CRISPR
    Mutation
    The second base of the first intron of B2M (From …
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    215546-plasmid-map-sequence-id-425336

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  19. B2M-SDMutation-EPG

    Plasmid
    #215545
    Purpose
    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
    Depositor
    Kai Wang
    Article
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Insert
    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived puromycin-GFP selection cassette (B2M Human)
    Use
    CRISPR
    Mutation
    The second base of the first intron of B2M (From …
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    215545-plasmid-map-sequence-id-425338

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Showing: 2421 - 2440 of 2467 results